| 质粒名称: | vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 160042 |
| 存储实验室: | Michael Elowitz |
|
载体骨架基本信息 |
|
| 载体名称: | PiggyBac |
| 载体出品公司: | SYSTEM BIOSCIENCE |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | 杀稻瘟菌素 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 30 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | osTIR1 |
| 物种来源: | Oryza Sativa (Asian rice) |
| 插入大小: | 1725 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR was a gift from Michael Elowitz (Addgene plasmid # 160042 ; http://n2t.net/addgene:160042 ; RRID:Addgene_160042) |
| 参考文献部分: | Synthetic mammalian signaling circuits for robust cell population control. Ma Y, Budde MW, Mayalu MN, Zhu J, Lu AC, Murray RM, Elowitz MB. Cell. 2022 Mar 17;185(6):967-979.e12. doi: 10.1016/j.cell.2022.01.026. Epub 2022 Mar 1. 10.1016/j.cell.2022.01.026 PubMed 35235768 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR 8930 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION For the auxin receiver that report on auxin..
ACCESSION .
VERSION .
KEYWORDS vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8930)
TITLE Synthetic mammalian signaling circuits for robust cell population
control
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8930)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8930
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(168..756)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(248..267)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(927..1787)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1550..1569
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1788..1892)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1918..2373)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2055..2076)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2267..2286
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2500..2522
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2514..2531
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 2515..2531
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
repeat_region 2661..2723
/label=piggyBac right (3') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
promoter 3166..4344
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 3397..4335
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 4292..4312
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 4367..6091
/codon_start=1
/product="rice transport inhibitor response 1 protein"
/label=OsTIR1
/note="F-box protein that mediates auxin-dependent
interaction of a ubiquitin ligase with an auxin-inducible
degron (Tan et al., 2007)"
/translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
CDS 6092..6145
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 6146..6850
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind complement(6288..6306)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 6556..6575
/label=mCherry-F
/note="mCherry, forward primer"
CDS 6872..7075
/codon_start=1
/product="minimal degron from the auxin-responsive IAA17
protein of Arabidopsis thaliana (Kubota et al., 2013)"
/label=mini-AID
/note="acts as an auxin-inducible degron (AID) in the
presence of TIR1"
/translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
AAFVKVSMDGAPYLRKIDLRMYK"
CDS 7088..7111
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 7127..7525
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
polyA_signal 7527..7648
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7564..7583)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7618..7637
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
repeat_region 8171..8205
/label=piggyBac left (5') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
primer_bind complement(8696..8712)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8696..8712)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8709..8731)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8720..8736
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8744..8774)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8789..8810
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8927..14)
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
61 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
121 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
181 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
241 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
301 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
361 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
421 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
481 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
541 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
601 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
661 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
721 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
781 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
841 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
901 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
961 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
1021 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga tccacgctca
1081 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
1141 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
1201 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
1261 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
1321 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
1381 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
1441 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
1501 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
1561 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
1621 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
1681 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
1741 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
1801 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
1861 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
1921 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
1981 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
2041 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
2101 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
2161 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
2221 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
2281 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
2341 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
2401 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
2461 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
2521 cgacggccag tgagcgcgcc tcgttcattc acgtttttga acccgtggag gacgggcaga
2581 ctcgcggtgc aaatgtgttt tacagcgtga tggagcagat gaagatgctc gacacgctgc
2641 agaacacgca gctagattaa ccctagaaag ataatcatat tgtgacgtac gttaaagata
2701 atcatgtgta aaattgacgc atgtgtttta tcggtctgta tatcgaggtt tatttattaa
2761 tttgaataga tattaagttt tattatattt acacttacat actaataata aattcaacaa
2821 acaatttatt tatgtttatt tatttattaa aaaaaacaaa aactcaaaat ttcttctata
2881 aagtaacaaa acttttatga gggacagccc ccccccaaag cccccaggga tgtaattacg
2941 tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc
3001 cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg
3061 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat
3121 acggggaaaa ggcctccacg gccagcacct gcgtgacgta cgtgaggctc cggtgcccgt
3181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
3241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
3301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
3361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
3421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
3481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
3541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
3601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
3661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
3721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
3781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
3841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
3901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
3961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
4021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
4081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
4141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
4201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
4261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
4321 tttcttccat ttcaggtgtc gtgaaccttc acgcaggtgg gccaccatga catactttcc
4381 tgaagaggtc gtcgaacaca tttttagctt cctgcctgca cagagagata gaaacacagt
4441 gagcctggtc tgcaaagtgt ggtacgagat cgaacgcctg agccggagag gagtgttcgt
4501 cggcaactgc tatgctgtga gagcaggcag ggtcgccgct aggtttccaa atgtgcgcgc
4561 actgaccgtc aaggggaaac cccacttcgc cgactttaac ctggtgcccc ctgattgggg
4621 aggatacgcc ggcccttgga tcgaggcagc cgctcgcggc tgtcatggac tggaggaact
4681 gcgcatgaag cgaatggtgg tctctgacga aagtctggag ctgctggctc ggagcttccc
4741 taggtttcgc gcactggtgc tgatttcttg cgaaggcttc agcaccgatg gactggcagc
4801 cgtggcctcc cactgtaagc tgctgcggga gctggacctc caggagaatg aagtggagga
4861 tagaggcccc agatggctgt cttgcttccc agactcatgt accagcctgg tgtccctgaa
4921 ctttgcctgc atcaaaggcg aagtgaatgc tgggtccctg gagcggctgg tctcaagaag
4981 ccccaacctg aggtctctgc ggctgaaccg gagcgtgagc gtggacactc tggctaagat
5041 tctgctgaga acccctaacc tggaggatct gggaaccggc aatctgacag acgatttcca
5101 gacagaatcc tactttaaac tgacttctgc cctggagaag tgtaaaatgc tgaggagtct
5161 gtcaggattc tgggatgctt cacccgtgtg cctgagcttt atctaccctc tgtgtgcaca
5221 gctgacaggc ctgaacctga gctatgcacc aaccctggac gccagtgatc tgacaaagat
5281 gatctcacgc tgcgtgaaac tccagcgact gtgggtgctg gactgtattt ccgataaggg
5341 gctccaggtg gtcgccagct cctgcaagga cctccaggag ctgagagtgt tcccatctga
5401 tttttacgtg gccggatata gtgctgtcac tgaggaaggc ctggtggcag tctcactggg
5461 atgcccaaag ctgaacagcc tgctgtattt ctgtcatcag atgactaatg ctgcactggt
5521 gaccgtcgcc aagaactgcc ctaatttcac ccgatttcgg ctgtgtattc tggaaccagg
5581 caaacccgac gtggtcacat cccagccact ggatgaaggg tttggagcta tcgtgagaga
5641 gtgcaaggga ctccagaggc tgagcatttc cggcctgctg acagacaaag tgttcatgta
5701 catcggcaag tatgctaagc agctggagat gctgagcatt gcatttgccg gagactccga
5761 taagggcatg atgcacgtga tgaacgggtg taagaatctg cgaaaactgg aaatccggga
5821 cagccctttc ggggatgccg ctctgctggg aaactttgcc agatacgaga caatgaggag
5881 cctgtggatg tctagttgca atgtgactct gaagggctgt caggtcctgg ctagtaaaat
5941 gcctatgctg aacgtggaag tcattaatga gcgggacggg tctaacgaaa tggaggaaaa
6001 tcatggcgac ctgccaaagg tggagaaact gtatgtgtat cggaccaccg caggggcaag
6061 agatgatgct cccaactttg tgaagattct ggagggcagg gggagcctcc taacttgcgg
6121 agacgtcgag gagaatcctg gcccagtgag caagggcgag gaggataaca tggccatcat
6181 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
6241 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
6301 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
6361 ctccaaggcc tacgtgaagc accccgccga cattcccgac tacttgaagc tgtccttccc
6421 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
6481 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
6541 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
6601 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
6661 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
6721 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
6781 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
6841 gctgtacaag tctggatccg gtgcaggcgc caaggagaag agtgcttgtc ctaaagatcc
6901 agccaaacct ccggccaagg cacaagttgt gggatggcca ccggtgagat cataccggaa
6961 gaacgtgatg gtttcctgcc aaaaatcaag cggtggcccg gaggcggcgg cgttcgtgaa
7021 ggtatcaatg gacggagcac cgtacttgag gaaaatcgat ttgaggatgt ataaaggagg
7081 atccggtgac tataaggacg atgacgacaa gggtggtgga gggtccatgg ccaagccttt
7141 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
7201 ctctgaagat tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
7261 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
7321 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
7381 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
7441 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
7501 ctctggttat gtgtgggagg gctgacaact tgtttattgc agcttataat ggttacaaat
7561 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
7621 gtttgtccaa actcatcaat gtatcttagg cccattggta tggctttttc cccgtatccc
7681 cccaggtgtc tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcccgtgc
7741 caccttcccc gtgcccgggc tgtccccgca cgctgccggc tcggggatgc ggggggagcg
7801 ccggaccgga gcggagcccc gggcggctcg ctgctgcccc ctagcggggg agggacgtaa
7861 ttacatccct gggggctttg ggggggggct gtccctgata tctataacaa gaaaatatat
7921 atataataag ttatcacgta agtagaacat gaaataacaa tataattatc gtatgagtta
7981 aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg gtcgttatag
8041 ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct ccaagcggcg
8101 actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag agagcaatat
8161 ttcaagaatg catgcgtcaa ttttacgcag actatctttc tagggttaat ctagctgcat
8221 caggatcata tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg gcgctatctg
8281 ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag
8341 tttgccgagg atgactgctg ctgcattgac gttgagcgaa aacgcacgtt taccatgatg
8401 attcgggaag gtgtggccat gcacgccttt aacggtgaac tgttcgttca ggccacctgg
8461 gataccagtt cgtcgcggct tttccggaca cagttccgga tggtcagccc gaagcgcatc
8521 agcaacccga acaataccgg cgacagccgg aactgccgtg ccggtgtgca gattaatgac
8581 agcggtgcgg cgctgggata ttacgtcagc gaggacgggt atcctggctg gatgccgcag
8641 aaatggacat ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat
8701 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
8761 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
8821 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
8881 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
//
