| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141377 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | 8955 bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-nsp11 |
| 物种来源: | SARS-CoV-2-nsp11 |
| 基因突变: | human codon optimized |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (C terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141377 ; http://n2t.net/addgene:141377 ; RRID:Addgene_141377) |
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-Puro 8955 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8955)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 8955)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8955
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..1182
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 231..1173
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1130..1150
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 1273..1296
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 1333..1356
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 1368..1941
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1535..1552)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 1762..1781
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 1974..2573
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1974..1993)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2470..2490
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 2587..3175
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2640..2660)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3058..3069)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3382..4015
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(4143..4159)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4143..4159)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4156..4178)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4167..4183
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4191..4221)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4236..4257
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4374..4391)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4545..5133)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4625..4644)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5304..6164)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5927..5946
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6165..6269)
/gene="bla"
/label=AmpR promoter
polyA_signal 6317..6451
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6354..6373)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6408..6427
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 6619..7252
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 7299..7424
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 7921..8154
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8339..8383
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 8646..8761
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
ORIGIN
1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatctat ttccggtgaa
1201 ttcgccgcca ccatgtcagc cgatgcgcag tctttcttga acggtttcgc cgtgctcgaa
1261 ggcggcgggg gatggagcca tccacaattc gagaaaggcg gtggttcagg aggaggtagc
1321 gggggtggat catggtcaca tccgcagttt gaaaagtaag gatcccgccc ctctccctcc
1381 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
1441 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
1501 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
1561 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
1621 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
1681 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
1741 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
1801 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
1861 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
1921 ctttgaaaaa cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg
1981 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
2041 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
2101 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
2161 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
2221 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
2281 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
2341 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
2401 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
2461 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
2521 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tagacgcgtc
2581 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
2641 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
2701 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
2761 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
2821 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
2881 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
2941 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
3001 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
3061 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
3121 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
3181 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
3241 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
3301 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
3361 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc
3421 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
3481 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
3541 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
3601 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
3661 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
3721 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
3781 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
3841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
3901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
3961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
4021 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
4081 gagaggcctt gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca
4141 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
4201 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
4261 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
4321 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
4381 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
4441 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
4501 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
4561 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
4621 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
4681 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
4741 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
4801 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
4861 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
4921 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
4981 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
5041 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
5101 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
5161 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
5221 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
5281 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
5341 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
5401 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
5461 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
5521 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
5581 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
5641 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
5701 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
5761 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
5821 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
5881 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
5941 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
6001 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
6061 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
6121 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
6181 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
6241 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
6301 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
6361 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
6421 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
6481 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
6541 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
6601 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
6661 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
6721 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
6781 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
6841 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
6901 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
6961 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
7021 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
7081 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
7141 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
7201 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
7261 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
7321 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
7381 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
7441 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
7501 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
7561 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
7621 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
7681 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
7741 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
7801 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
7861 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
7921 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
7981 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
8041 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
8101 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
8161 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
8221 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
8281 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
8341 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
8401 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
8461 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
8521 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
8581 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
8641 tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
8701 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
8761 ttcgggttta ttacagggac agcagagatc cagtttatcg atgagtaatt catacaaaag
8821 gactcgcccc tgccttgggg aatcccaggg accgtcgtta aactcccact aacgtagaac
8881 ccagagatcg ctgcgttccc gccccctcac ccgcccgctc tcgtcatcac tgaggtggag
8941 aagagcatgc gtgag
//
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