pEcCpf1

LOCUS       pEcCpf1                14479 bp    DNA     circular SYN 24-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 18, 2025 from SnapGene 8.1.0
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..14479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(107..1057)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the
                     pSC101 origin"
                     /label=rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      complement(1105..1327)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(1832..2647)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7027..7905)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        7932..8216
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             8260..8676
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the
                     lambda Red system"
                     /label=gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             8682..9467
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             9464..10144
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the
                     lambda Red system"
                     /label=exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     CDS             10726..12147
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     misc_RNA        complement(12270..12345)
                     /label=g RNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(12445..12563)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             12709..13545
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter"
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     CDS             13619..14467
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
ORIGIN
        1 catgggtatg gacagttttc cctttgatat gtaacggtga acagttgttc tacttttgtt
       61 tgttagtctt gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt
      121 atttagccag tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac
      181 cattgagatc atgcttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct
      241 gaatttttgc agttaaagca tcgtgtagtg tttttcttag tccgttacgt aggtaggaat
      301 ctgatgtaat ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt
      361 cggttacgag atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc
      421 ggcctcgctt atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg
      481 gtttcaaaac ccattggtta agccttttaa actcatggta gttattttca agcattaaca
      541 tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta
      601 gttcttttaa taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat
      661 gttccagatt atattttatg aattttttta actggaaaag ataaggcaat atctcttcac
      721 taaaaactaa ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct
      781 caaagccttt aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg
      841 ctttagctaa tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt
      901 tataagtgaa cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta
      961 gtgccacaca gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg
     1021 ctagttcatt tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat
     1081 tttaatcact ataccaattg agatgggcta gtcaatgata attactagtc cttttccttt
     1141 gagttgtggg tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta
     1201 gaccctctgt aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta
     1261 taatttatag aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt
     1321 ataactcact actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg
     1381 ctcctctaca aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg
     1441 ttgcggccgc aatcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     1501 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     1561 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     1621 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     1681 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     1741 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     1801 cccactgcag caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa
     1861 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     1921 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     1981 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     2041 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat
     2101 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     2161 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     2221 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     2281 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     2341 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     2401 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     2461 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     2521 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     2581 aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat
     2641 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
     2701 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttccc
     2761 tgcagctgaa caatacttaa tactatagaa tgataacaaa ataaactact ttttaaaaga
     2821 attttgtgtt ataatctatt tattattaag tattgggtaa tattttttga agagatattt
     2881 tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa ctattattga
     2941 aatcatcaaa ctcattatgg atttaattta aactttttat tttaggaggc aaaaatgtca
     3001 atttatcaag aatttgttaa taaatatagt ttaagtaaaa ctctaagatt tgagttaatc
     3061 ccacagggta aaacacttga aaacataaaa gcaagaggtt tgattttaga tgatgagaaa
     3121 agagctaaag actacaaaaa ggctaaacaa ataattgata aatatcatca gttttttata
     3181 gaggagatat taagttcggt ttgtattagc gaagatttat tacaaaacta ttctgatgtt
     3241 tattttaaac ttaaaaagag tgatgatgat aatctacaaa aagattttaa aagtgcaaaa
     3301 gatacgataa agaaacaaat atctgaatat ataaaggact cagagaaatt taagaatttg
     3361 tttaatcaaa accttatcga tgctaaaaaa gggcaagagt cagatttaat tctatggcta
     3421 aagcaatcta aggataatgg tatagaacta tttaaagcca atagtgatat cacagatata
     3481 gatgaggcgt tagaaataat caaatctttt aaaggttgga caacttattt taagggtttt
     3541 catgaaaata gaaaaaatgt ttatagtagc aatgatattc ctacatctat tatttatagg
     3601 atagtagatg ataatttgcc taaatttcta gaaaataaag ctaagtatga gagtttaaaa
     3661 gacaaagctc cagaagctat aaactatgaa caaattaaaa aagatttggc agaagagcta
     3721 acctttgata ttgactacaa aacatctgaa gttaatcaaa gagttttttc acttgatgaa
     3781 gtttttgaga tagcaaactt taataattat ctaaatcaaa gtggtattac taaatttaat
     3841 actattattg gtggtaaatt tgtaaatggt gaaaatacaa agagaaaagg tataaatgaa
     3901 tatataaatc tatactcaca gcaaataaat gataaaacac tcaaaaaata taaaatgagt
     3961 gttttattta agcaaatttt aagtgataca gaatctaaat cttttgtaat tgataagtta
     4021 gaagatgata gtgatgtagt tacaacgatg caaagttttt atgagcaaat agcagctttt
     4081 aaaacagtag aagaaaaatc tattaaagaa acactatctt tattatttga tgatttaaaa
     4141 gctcaaaaac ttgatttgag taaaatttat tttaaaaatg ataaatctct tactgatcta
     4201 tcacaacaag tttttgatga ttatagtgtt attggtacag cggtactaga atatataact
     4261 caacaaatag cacctaaaaa tcttgataac cctagtaaga aagagcaaga attaatagcc
     4321 aaaaaaactg aaaaagcaaa atacttatct ctagaaacta taaagcttgc cttagaagaa
     4381 tttaataagc atagagatat agataaacag tgtaggtttg aagaaatact tgcaaacttt
     4441 gcggctattc cgatgatatt tgatgaaata gctcaaaaca aagacaattt ggcacagata
     4501 tctatcaaat atcaaaatca aggtaaaaaa gacctacttc aagctagtgc ggaagatgat
     4561 gttaaagcta tcaaggatct tttagatcaa actaataatc tcttacataa actaaaaata
     4621 tttcatatta gtcagtcaga agataaggca aatattttag acaaggatga gcatttttat
     4681 ctagtatttg aggagtgcta ctttgagcta gcgaatatag tgcctcttta taacaaaatt
     4741 agaaactata taactcaaaa gccatatagt gatgagaaat ttaagctcaa ttttgagaac
     4801 tcgactttgg ctaatggttg ggataaaaat aaagagcctg acaatacggc aattttattt
     4861 atcaaagatg ataaatatta tctgggtgtg atgaataaga aaaataacaa aatatttgat
     4921 gataaagcta tcaaagaaaa taaaggcgag ggttataaaa aaattgttta taaactttta
     4981 cctggcgcaa ataaaatgtt acctaaggtt ttcttttctg ctaaatctat aaaattttat
     5041 aatcctagtg aagatatact tagaataaga aatcattcca cacatacaaa aaatggtagt
     5101 cctcaaaaag gatatgaaaa atttgagttt aatattgaag attgccgaaa atttatagat
     5161 ttttataaac agtctataag taagcatccg gagtggaaag attttggatt tagattttct
     5221 gatactcaaa gatataattc tatagatgaa ttttatagag aagttgaaaa tcaaggctac
     5281 aaactaactt ttgaaaatat atcagagagc tatattgata gcgtagttaa tcagggtaaa
     5341 ttgtacctat tccaaatcta taataaagat ttttcagctt atagcaaagg gcgaccaaat
     5401 ctacatactt tatattggaa agcgctgttt gatgagagaa atcttcaaga tgtggtttat
     5461 aagctaaatg gtgaggcaga gcttttttat cgtaaacaat caatacctaa aaaaatcact
     5521 cacccagcta aagaggcaat agctaataaa aacaaagata atcctaaaaa agagagtgtt
     5581 tttgaatatg atttaatcaa agataaacgc tttactgaag ataagttttt ctttcactgt
     5641 cctattacaa tcaattttaa atctagtgga gctaataagt ttaatgatga aatcaattta
     5701 ttgctaaaag aaaaagcaaa tgatgttcat atattaagta tagatagagg tgaaagacat
     5761 ttagcttact atactttggt agatggtaaa ggcaatatca tcaaacaaga tactttcaac
     5821 atcattggta atgatagaat gaaaacaaac taccatgata agcttgctgc aatagagaaa
     5881 gatagggatt cagctaggaa agactggaaa aagataaata acatcaaaga gatgaaagag
     5941 ggctatctat ctcaggtagt tcatgaaata gctaagctag ttatagagta taatgctatt
     6001 gtggtttttg aggatttaaa ttttggattt aaaagagggc gtttcaaggt agagaagcag
     6061 gtctatcaaa agttagaaaa aatgctaatt gagaaactaa actatctagt tttcaaagat
     6121 aatgagtttg ataaaactgg gggagtgctt agagcttatc agctaacagc accttttgag
     6181 acttttaaaa agatgggtaa acaaacaggt attatctact atgtaccagc tggttttact
     6241 tcaaaaattt gtcctgtaac tggttttgta aatcagttat atcctaagta tgaaagtgtc
     6301 agcaaatctc aagagttctt tagtaagttt gacaagattt gttataacct tgataagggc
     6361 tattttgagt ttagttttga ttataaaaac tttggtgaca aggctgccaa aggcaagtgg
     6421 actatagcta gctttgggag tagattgatt aactttagaa attcagataa aaatcataat
     6481 tgggatactc gagaagttta tccaactaaa gagttggaga aattgctaaa agattattct
     6541 atcgaatatg ggcatggcga atgtatcaaa gcagctattt gcggtgagag cgacaaaaag
     6601 ttttttgcta agctaactag tgtcctaaat actatcttac aaatgcgtaa ctcaaaaaca
     6661 ggtactgagt tagattatct aatttcacca gtagcagatg taaatggcaa tttctttgat
     6721 tcgcgacagg cgccaaaaaa tatgcctcaa gatgctgatg ccaatggtgc ttatcatatt
     6781 gggctaaaag gtctgatgct actaggtagg atcaaaaata atcaagaggg caaaaaactc
     6841 aatttggtta tcaaaaatga agagtatttt gagttcgtgc agaataggaa taactaaagt
     6901 atattttaga tgaagattat ttcttaatct agacatgagc ggatacatat ttgaatgtat
     6961 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgcatc
     7021 gatttattat gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa
     7081 ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaagtaga gttgatcgtc
     7141 aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt
     7201 cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg
     7261 gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc
     7321 aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc
     7381 catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag
     7441 cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg
     7501 cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt
     7561 ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac
     7621 ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg
     7681 cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac
     7741 cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat
     7801 aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt
     7861 aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc
     7921 gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt
     7981 ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca
     8041 aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa
     8101 agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat
     8161 aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc
     8221 gtttttttgg gaattcgagc tctaaggagg ttataaaaaa tggatattaa tactgaaact
     8281 gagatcaagc aaaagcattc actaaccccc tttcctgttt tcctaatcag cccggcattt
     8341 cgcgggcgat attttcacag ctatttcagg agttcagcca tgaacgctta ttacattcag
     8401 gatcgtcttg aggctcagag ctgggcgcgt cactaccagc agctcgcccg tgaagagaaa
     8461 gaggcagaac tggcagacga catggaaaaa ggcctgcccc agcacctgtt tgaatcgcta
     8521 tgcatcgatc atttgcaacg ccacggggcc agcaaaaaat ccattacccg tgcgtttgat
     8581 gacgatgttg agtttcagga gcgcatggca gaacacatcc ggtacatggt tgaaaccatt
     8641 gctcaccacc aggttgatat tgattcagag gtataaaacg aatgagtact gcactcgcaa
     8701 cgctggctgg gaagctggct gaacgtgtcg gcatggattc tgtcgaccca caggaactga
     8761 tcaccactct tcgccagacg gcatttaaag gtgatgccag cgatgcgcag ttcatcgcat
     8821 tactgatcgt tgccaaccag tacggcctta atccgtggac gaaagaaatt tacgcctttc
     8881 ctgataagca gaatggcatc gttccggtgg tgggcgttga tggctggtcc cgcatcatca
     8941 atgaaaacca gcagtttgat ggcatggact ttgagcagga caatgaatcc tgtacatgcc
     9001 ggatttaccg caaggaccgt aatcatccga tctgcgttac cgaatggatg gatgaatgcc
     9061 gccgcgaacc attcaaaact cgcgaaggca gagaaatcac ggggccgtgg cagtcgcatc
     9121 ccaaacggat gttacgtcat aaagccatga ttcagtgtgc ccgtctggcc ttcggatttg
     9181 ctggtatcta tgacaaggat gaagccgagc gcattgtcga aaatactgca tacactgcag
     9241 aacgtcagcc ggaacgcgac atcactccgg ttaacgatga aaccatgcag gagattaaca
     9301 ctctgctgat cgccctggat aaaacatggg atgacgactt attgccgctc tgttcccaga
     9361 tatttcgccg cgacattcgt gcatcgtcag aactgacaca ggccgaagca gtaaaagctc
     9421 ttggattcct gaaacagaaa gccgcagagc agaaggtggc agcatgacac cggacattat
     9481 cctgcagcgt accgggatcg atgtgagagc tgtcgaacag ggggatgatg cgtggcacaa
     9541 attacggctc ggcgtcatca ccgcttcaga agttcacaac gtgatagcaa aaccccgctc
     9601 cggaaagaag tggcctgaca tgaaaatgtc ctacttccac accctgcttg ctgaggtttg
     9661 caccggtgtg gctccggaag ttaacgctaa agcactggcc tggggaaaac agtacgagaa
     9721 cgacgccaga accctgtttg aattcacttc cggcgtgaat gttactgaat ccccgatcat
     9781 ctatcgcgac gaaagtatgc gtaccgcctg ctctcccgat ggtttatgca gtgacggcaa
     9841 cggccttgaa ctgaaatgcc cgtttacctc ccgggatttc atgaagttcc ggctcggtgg
     9901 tttcgaggcc ataaagtcag cttacatggc ccaggtgcag tacagcatgt gggtgacgcg
     9961 aaaaaatgcc tggtactttg ccaactatga cccgcgtatg aagcgtgaag gcctgcatta
    10021 tgtcgtgatt gagcgggatg aaaagtacat ggcgagtttt gacgagatcg tgccggagtt
    10081 catcgaaaaa atggacgagg cactggctga aattggtttt gtatttgggg agcaatggcg
    10141 atgacgcatc ctcacgataa tatccgggta ggcgcaatca ctttcgtcta ctccgttaca
    10201 aagcgaggct gggtatttcc cggcctttct gttatccgaa atccactgaa agcacagcgg
    10261 ctggctgagg agataaataa taaacgaggg gctgtatgca caaagcatct tctgttgagt
    10321 taagaacgag tatcgagatg gcacatagcc ttgctcaaat tggaatcagg tttgtgccaa
    10381 taccagtaga aacagacgaa gaatccatgg gtatggacag atcctcttta ggcccgtagt
    10441 ctgcaaatcc ttttatgatt ttctatcaaa caaaagagga aaatagacca gttgcaatcc
    10501 aaacgagagt ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt tactgataaa
    10561 gcaggcaaga cctaaaatgt gtaaagggca aagtgtatac tttggcgtca ccccttacat
    10621 attttaggtc tttttttatt gtgcgtaact aacttgccat cttcaaacag gagggctgga
    10681 agaagcagac cgctaacaca gtacataaaa aaggagacat gaacgatgaa catcaaaaag
    10741 tttgcaaaac aagcaacagt attaaccttt actaccgcac tgctggcagg aggcgcaact
    10801 caagcgtttg cgaaagaaac gaaccaaaag ccatataagg aaacatacgg catttcccat
    10861 attacacgcc atgatatgct gcaaatccct gaacagcaaa aaaatgaaaa atatcaagtt
    10921 cctgaattcg attcgtccac aattaaaaat atctcttctg caaaaggcct ggacgtttgg
    10981 gacagctggc cattacaaaa cgctgacggc actgtcgcaa actatcacgg ctaccacatc
    11041 gtctttgcat tagccggaga tcctaaaaat gcggatgaca catcgattta catgttctat
    11101 caaaaagtcg gcgaaacttc tattgacagc tggaaaaacg ctggccgcgt ctttaaagac
    11161 agcgacaaat tcgatgcaaa tgattctatc ctaaaagacc aaacacaaga atggtcaggt
    11221 tcagccacat ttacatctga cggaaaaatc cgtttattct acactgattt ctccggtaaa
    11281 cattacggca aacaaacact gacaactgca caagttaacg tatcagcatc agacagctct
    11341 ttgaacatca acggtgtaga ggattataaa tcaatctttg acggtgacgg aaaaacgtat
    11401 caaaatgtac agcagttcat cgatgaaggc aactacagct caggcgacaa ccatacgctg
    11461 agagatcctc actacgtaga agataaaggc cacaaatact tagtatttga agcaaacact
    11521 ggaactgaag atggctacca aggcgaagaa tctttattta acaaagcata ctatggcaaa
    11581 agcacatcat tcttccgtca agaaagtcaa aaacttctgc aaagcgataa aaaacgcacg
    11641 gctgagttag caaacggcgc tctcggtatg attgagctaa acgatgatta cacactgaaa
    11701 aaagtgatga aaccgctgat tgcatctaac acagtaacag atgaaattga acgcgcgaac
    11761 gtctttaaaa tgaacggcaa atggtacctg ttcactgact cccgcggatc aaaaatgacg
    11821 attgacggca ttacgtctaa cgatatttac atgcttggtt atgtttctaa ttctttaact
    11881 ggcccataca agccgctgaa caaaactggc cttgtgttaa aaatggatct tgatcctaac
    11941 gatgtaacct ttacttactc acacttcgct gtacctcaag cgaaaggaaa caatgtcgtg
    12001 attacaagct atatgacaaa cagaggattc tacgcagaca aacaatcaac gtttgcgcca
    12061 agcttcctgc tgaacatcaa aggcaagaaa acatctgttg tcaaagacag catccttgaa
    12121 caaggacaat taacagttaa caaataaaaa cgcaaaagaa aatgccgata tcctattggc
    12181 attttctttt atttcttatc aacataaagg tgaatcccat atgaactata taaaagcagg
    12241 caaatggcta accgtattcc tcaaaaaaag caccgactcg gtgccacttt ttcaagttga
    12301 taacggacta gccttatttt aacttgctat ttctagctct aaaacggttg gactcaagac
    12361 gatagtatct acaacagtag aaatttggct tcagcagagc gcagatacat ctacaacagt
    12421 agaaattatt taaagttctt agactacgac cagtctaaaa agcgcctgaa ttcgcgacct
    12481 tctcgttact gacaggaaaa tgggccattg gcaaccaggg aaagatgaac gtgatgatgt
    12541 tcacaatttg ctgaattgtg gtgatgtgat gctcaccgca tttcctgaaa attcacgctg
    12601 tatcttgaaa aatcgacgtt ttttacgtgg ttttccgtcg aaaatttaag gtaagaacct
    12661 gacctcgtga ttactatttc gccgtgttga cgacatcagg aggccagtat gaccgtatta
    12721 catagtgtgg atttttttcc gtctggtaac gcgtccgtgg cgatagaacc ccggctcccg
    12781 caggcggatt ttcctgaaca tcatcatgat tttcatgaaa ttgtgattgt cgaacatggc
    12841 acgggtattc atgtgtttaa tgggcagccc tataccatca ccggtggcac ggtctgtttc
    12901 gtacgcgatc atgatcggca tctgtatgaa cataccgata atctgtgtct gaccaatgtg
    12961 ctgtatcgct cgccggatcg atttcagttt ctcgccgggc tgaatcagtt gctgccacaa
    13021 gagctggatg ggcagtatcc gtctcactgg cgcgttaacc acagcgtatt gcagcaggtg
    13081 cgacagctgg ttgcacagat ggaacagcag gaaggggaaa atgatttacc ctcgaccgcc
    13141 agtcgcgaga tcttgtttat gcaattactg ctcttgctgc gtaaaagcag tttgcaggag
    13201 aacctggaaa acagcgcatc acgtctcaac ttgcttctgg cctggctgga ggaccatttt
    13261 gccgatgagg tgaattggga tgccgtggcg gatcaatttt ctctttcact gcgtacgcta
    13321 catcggcagc ttaagcagca aacgggactg acgcctcagc gatacctgaa ccgcctgcga
    13381 ctgatgaaag cccgacatct gctacgccac agcgaggcca gcgttactga catcgcctat
    13441 cgctgtggat tcagcgacag taaccacttt tcgacgcttt ttcgccgaga gtttaactgg
    13501 tcaccgcgtg atattcgcca gggacgggat ggctttctgc aataacgcga atcttctcaa
    13561 cgtatttgta cgccatattg cgaataatca acttcgttct ctggccgagg tagccacggt
    13621 ggcgcatcag ttaaaacttc tcaaagatga tttttttgcc agcgaccagc aggcagtcgc
    13681 tgtggctgac cgttatccgc aagatgtctt tgctgaacat acacatgatt tttgtgagct
    13741 ggtgattgtc tggcgcggta atggcctgca tgtactcaac gatcgccctt atcgcattac
    13801 ccgtggcgat ctcttttaca ttcatgctga cgataaacac tcctacgctt ccgttaacga
    13861 tctggttttg cagaatatta tttattgccc ggagcgtctg aagctgaatc ttgactggca
    13921 gggggcgatt ccgggattta acgccagcgc agggcaacca cactggcgct taggtagcat
    13981 ggggatggcg caggcgcggc aggttatcgg tcagcttgag catgaaagta gtcagcatgt
    14041 gccgtttgct aacgaaatgg ctgagttgct gttcgggcag ttggtgatgt tgctgaatcg
    14101 ccatcgttac accagtgatt cgttgccgcc aacatccagc gaaacgttgc tggataagct
    14161 gattacccgg ctggcggcta gcctgaaaag tccctttgcg ctggataaat tttgtgatga
    14221 ggcatcgtgc agtgagcgcg ttttgcgtca gcaatttcgc cagcagactg gaatgaccat
    14281 caatcaatat ctgcgacagg tcagagtgtg tcatgcgcaa tatcttctcc agcatagccg
    14341 cctgttaatc agtgatattt cgaccgaatg tggctttgaa gatagtaact atttttcggt
    14401 ggtgtttacc cgggaaaccg ggatgacgcc cagccagtgg cgtcatctca attcgcagaa
    14461 agattaattc gccatgccc
//