| 出品公司: | Takara |
|---|---|
| 载体名称: | pG-Tf2 |
| 质粒类型: | Chaperone Plasmid分子伴侣质粒 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8.3kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | chloromycetin |
| 筛选标记: | Tetracycline |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
买家导航
pG-Tf2载体质粒基本信息
pG-Tf2质粒图谱载体图谱和pG-Tf2载体序列质粒序列多克隆位点信息
pG-Tf2质粒载体简介
pG-Tf2质粒序列载体序列
LOCUS pG-Tf2 8290 bp ds-DNA circular SYN 13-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8290)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, September 13, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..8290
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 30..47
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
RBS 98..109
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 206..259
/codon_start=1
/product="GroES chaperone mobile loop that interacts with
GroEL"
/note="S loop"
/translation="EVETKSAGGIVLTGSAAA"
terminator 4387..4473
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
promoter 4533..4588
/gene="tetR"
/note="tetR/tetA promoters"
/note="overlapping promoters for bacterial tetR and tetA"
protein_bind 4534..4552
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 4564..4582
/bound_moiety="tetracycline repressor TetR"
/note="tet operator"
/note="bacterial operator O1 for the tetR and tetA genes"
CDS 4607..5230
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/note="TetR"
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
CDS complement(6245..6904)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
promoter complement(6905..7007)
/note="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
rep_origin complement(7533..8077)
/direction=LEFT
/note="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt ccctatcagt gatagagtat aatagagtcg
61 aattgttagc ggagaagaat ttcacacaga attcattaaa gaggagaaag tcgaggcgtc
121 acccataaca gatacggact ttctcaaagg agagttatca atgaatattc gtccattgca
181 tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa tctgctggcg gcatcgttct
241 gaccggctct gcagcggcta aatccacccg cggcgaagtg ctggctgtcg gcaatggccg
301 tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa gttggcgaca tcgttatttt
361 caacgatggc tacggtgtga aatctgagaa gatcgacaat gaagaagtgt tgatcatgtc
421 cgaaagcgac attctggcaa ttgttgaagc gtaatccgcg cacgacactg aacatacgaa
481 tttaaggaat aaagataatg gcagctaaag acgtaaaatt cggtaacgac gctcgtgtga
541 aaatgctgcg cggcgtaaac gtactggcag atgcagtgaa agttaccctc ggtccaaaag
601 gccgtaacgt agttctggat aaatctttcg gtgcaccgac catcaccaaa gatggtgttt
661 ccgttgctcg tgaaatcgaa ctggaagaca agttcgaaaa tatgggtgcg cagatggtga
721 aagaagttgc ctctaaagca aacgacgctg caggcgacgg taccaccact gcaaccgtac
781 tggctcaggc tatcatcact gaaggtctga aagctgttgc tgcgggcatg aacccgatgg
841 acctgaaacg tggtatcgac aaagcggtta ccgctgcagt tgaagaactg aaagcgctgt
901 ccgtaccatg ctctgactct aaagcgattg ctcaggttgg taccatctcc gctaactccg
961 acgaaaccgt aggtaaactg atcgctgaag cgatggacaa agtcggtaaa gaaggcgtta
1021 tcaccgttga agacggtacc ggtctgcagg acgaactgga cgtggttgaa ggtatgcagt
1081 tcgaccgtgg ctacctgtct ccttacttca tcaacaagcc ggaaactggc gcagtagaac
1141 tggaaagccc gttcatcctg ctggctgaca agaaaatctc caacatccgc gaaatgctgc
1201 cggttctgga agctgttgcc aaagcaggca aaccgctgct gatcatcgct gaagatgtag
1261 aaggcgaagc gctggcaact ctggttgtta acaccatgcg tggcatcgtg aaagtcgctg
1321 cggttaaagc accgggcttc ggcgatcgtc gtaaagctat gctgcaggat atcgcaaccc
1381 tgactggcgg taccgtgatc tctgaagaga tcggtatgga gctggaaaaa gcaaccctgg
1441 aagacctggg tcaggctaaa cgtgttgtga tcaacaaaga caccaccact atcatcgatg
1501 gcgtgggtga agaagctgca atccagggcc gtgttgctca gatccgtcag cagattgaag
1561 aagcaacttc tgactacgac cgtgaaaaac tgcaggaacg cgtagcgaaa ctggcaggcg
1621 gcgttgcagt tatcaaagtg ggtgctgcta ccgaagttga aatgaaagag aaaaaagcac
1681 gcgttgaaga tgccctgcac gcgacccgtg ctgcggtaga agaaggcgtg gttgctggtg
1741 gtggtgttgc gctgatccgc gtagcgtcta aactggctga cctgcgtggt cagaacgaag
1801 accagaacgt gggtatcaaa gttgcactgc gtgcaatgga agctccgctg cgtcagatcg
1861 tattgaactg cggcgaagaa ccgtctgttg ttgctaacac cgttaaaggc ggcgacggca
1921 actacggtta caacgcagca accgaagaat acggcaacat gatcgacatg ggtatcctgg
1981 atccaaccaa agtaactcgt tctgctctgc agtacgcagc ttctgtggct ggcctgatga
2041 tcaccaccga atgcatggtt accgacctgc cgaaaaacga tgcagctgac ttaggcgctg
2101 ctggcggtat gggcggcatg ggtggcatgg gcggcatgat gtaattgccc tgcacctcgc
2161 agaaataaac aaacccccct gtgatttttt gaggtaacaa gatgcaagtt tcagttgaaa
2221 ccactcaagg ccttggccgc cgtgtaacga ttactatcgc tgctgacagc atcgagaccg
2281 ctgttaaaag cgagctggtc aacgttgcga aaaaagtacg tattgacggc ttccgcaaag
2341 gcaaagtgcc aatgaatatc gttgctcagc gttatggcgc gtctgtacgc caggacgttc
2401 tgggtgacct gatgagccgt aacttcattg acgccatcat taaagaaaaa atcaatccgg
2461 ctggcgcacc gacttatgtt ccgggcgaat acaagctggg tgaagacttc acttactctg
2521 tagagtttga agtttatccg gaagttgaac tgcagggtct ggaagcgatc gaagttgaaa
2581 aaccgatcgt tgaagtgacc gacgctgacg ttgacggcat gctggatact ctgcgtaaac
2641 agcaggcgac ctggaaagaa aaagacggcg ctgttgaagc agaagaccgc gtaaccatcg
2701 acttcaccgg ttctgtagac ggcgaagagt tcgaaggcgg taaagcgtct gatttcgtac
2761 tggcgatggg ccagggtcgt atgatcccgg gctttgaaga cggtatcaaa ggccacaaag
2821 ctggcgaaga gttcaccatc gacgtgacct tcccggaaga ataccacgca gaaaacctga
2881 aaggtaaagc agcgaaattc gctatcaacc tgaagaaagt tgaagagcgt gaactgccgg
2941 aactgactgc agaattcatc aaacgtttcg gcgttgaaga tggttccgta gaaggtctgc
3001 gcgctgaagt gcgtaaaaac atggagcgcg agctgaagag cgccatccgt aaccgcgtta
3061 agtctcaggc gatcgaaggt ctggtaaaag ctaacgacat cgacgtaccg gctgcgctga
3121 tcgacagcga aatcgacgtt ctgcgtcgcc aggctgcaca gcgtttcggt ggcaacgaaa
3181 aacaagctct ggaactgccg cgcgaactgt tcgaagaaca ggctaaacgc cgcgtagttg
3241 ttggcctgct gctgggcgaa gttatccgca ccaacgagct gaaagctgac gaagagcgcg
3301 tgaaaggcct gatcgaagag atggcttctg cgtacgaaga tccgaaagaa gttatcgagt
3361 tctacagcaa aaacaaagaa ctgatggaca acatgcgcaa tgttgctctg gaagaacagg
3421 ctgttgaagc tgtactggcg aaagcgaaag tgactgaaaa agaaaccact ttcaacgagc
3481 tgatgaacca gcaggcgtaa tttacgcagc ataacgcgct aaattcgcac aaaggcccgt
3541 caccgccagg tggtgggctt ttttttgtca tgttaaggaa cgcgtcattt ttctgactgg
3601 ccaggttgaa gaccacatgg ctaacctgat tgtggcgcag atgctgttcc tggaagcaga
3661 aaacccagaa aaagatatct atctgtacat taactcccca ggcggggtga tcactgccgg
3721 gatgtctatc tatgacacca tgcagtttat caagcctgat gtcagcacca tctgtatggg
3781 ccaggcggcc tcgatgggcg ctttcttgct gaccgcaggg gcaaaaggta aacgtttttg
3841 cctgccgaat tcgcgcgtga tgattcacca accgttgggc ggctaccagg gccaggcgac
3901 cgatatcgaa attcatgccc gtgaaattct gaaagttaaa gggcgcatga atgaacttat
3961 ggcgcttcat acgggtcaat cattagaaca gattgaacgt gataccgagc gcgatcgctt
4021 cctttccgcc cctgaagcgg tggaatacgg tctggtcgat tcgattctga cccatcgtaa
4081 ttgatgccag aggcgcaact gtgccgctat acttatccag ggcggcacaa cgctgtaagc
4141 gcttgcgcct gagaatggca tttgcgtcgt cgtgtgcggc acaaagaaca aagaagaggt
4201 tttgacccat gacagataaa cgcaaagatg gctcaggcaa attgctgtat tgctcttttt
4261 gcggcaaaag ccagcatgaa gtgcgcaagc tgattgccgg tccatccgtg tatatctgcg
4321 acgaatgtgt tgatttatgt aacgacatca ttcggggcag aaatgtctgg gggtttctag
4381 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
4441 ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagaccta gggtcgactc
4501 tagaggatct actagtcata tggatttcac ttttctctat cactgatagg gagtggtaaa
4561 ataactctat caatgataga gtgtcaacaa aaattaggaa ttaatgatgt ctagattaga
4621 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
4681 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
4741 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
4801 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
4861 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
4921 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
4981 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
5041 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
5101 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
5161 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag
5221 tgggtcttaa aagcagcata acctttttcc gtgatggtaa cttcacggta accaagatgt
5281 cgagttaacc accctttaga ttcataaagc gaaaataatg cggctccaac gtacccacct
5341 aaatggaaac ggatcggatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
5401 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
5461 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
5521 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtccc
5581 ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc aaccggtgat accacgatac
5641 tatgactgag agtcaacgcc atgagcggcc tcatttctta ttctgagtta caacagtccg
5701 caccgctgtc cggtagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
5761 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgccc aacagtcccc
5821 cggccacggg gcctgccacc atacccacgc cgaaacaagc gccctgcacc attatgttcc
5881 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga
5941 agcgctaacc gtttttatca ggctctggga ggcagaataa atgatcatat cgtcaattat
6001 tacctccacg gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac
6061 actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg
6121 accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc
6181 ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa
6241 aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
6301 cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct
6361 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
6421 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
6481 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
6541 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
6601 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
6661 gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa
6721 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
6781 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
6841 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
6901 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
6961 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc
7021 gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt atttattctg
7081 cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca
7141 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc
7201 agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga
7261 catcagcgct agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg
7321 aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata
7381 caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
7441 agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa
7501 cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag
7561 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
7621 caggcgtttc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
7681 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
7741 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
7801 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg
7861 gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg
7921 ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
7981 agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc
8041 agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat
8101 aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc
8161 catacgatat aagttgtaat tctcatgttt gacagcttat catcgataag cttgcatgcc
8221 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
8281 tcgtcttcac
//
