| 载体名称: | pRSETB-mVenus |
|---|---|
| 质粒类型: | 大肠杆菌表达；黄色荧光蛋白表达质粒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | T7 |
| 载体大小: | 3.6 kb |
| 5’测序引物: | T7: TAATACGACTCACTATAGGG |
| 3’测序引物: | T7t: GCTAGTTATTGCTCAGCGG |
| 载体标签: | N-His |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | mVenus黄色荧光蛋白 |
| 克隆菌株: | DH5 alpha等 |
| 宿主细胞（系）: | 表达菌株是BL21(DE3) |
| 产品目录号: | V2316 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
| 备注: | 大肠杆菌表达黄色荧光蛋白质粒。 |
买家导航
pRSETB-mVenus载体质粒基本信息
pRSETB-mVenus质粒图谱载体图谱和pRSETB-mVenus载体序列质粒序列多克隆位点信息
pRSETB-mVenus质粒载体简介
pRSETB-mVenus质粒序列载体序列
LOCUS pRSETB-mVenus 3571 bp DNA circular SYN 24-AUG-2024
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3571)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Aug 24, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
https://www.snapgene.com
COMMENT VNTDATE|1066521600|
COMMENT VNTDBDATE|1066521600|
COMMENT VNTNAME|pRSETB-mVenus|
COMMENT VNTAUTHORNAME|Ming|
FEATURES Location/Qualifiers
promoter 20..38
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
RBS 70..92
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 112..129
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
CDS 133..165
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=T7 tag (gene 10 leader)
/note="promotes efficient translation in E. coli"
CDS 169..192
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/label=Xpress(TM) tag
CDS 199..918
/codon_start=1
/product="photostable derivative of mVenus (Lee et al.,
2020)"
/label=mGold
/note="mammalian codon-optimized"
terminator 986..1033
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 1130..1585
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1611..1715
/gene="bla"
/label=AmpR promoter
CDS 1716..2576
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
rep_origin 2747..3335
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat
121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
181 gatgacgata aggatccgat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
241 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
301 gagggcgatg ccacctacgg caagctgacc ctgaagttca tcagcaccac cggcaagctg
361 cccgtgccct ggcccaccct cgtgaccacc ctcggctacg gcctgcagtg cttcgcccgc
421 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
481 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
541 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
601 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc
661 gccgacaagc agaagaacgg catcaaggca aacttcaaga tccgccacaa catcgaggac
721 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
781 ctgctgcccg acaaccacta cctgagctac cagtccaaac tgagcaaaga ccccaacgag
841 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
901 gacgagctgt acaagtaagc ttgatccggc tgctaacaaa gcccgaaagg aagctgagtt
961 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
1021 gaggggtttt ttgctgaaag gaggaactat atccggatct ggcgtaatag cgaagaggcc
1081 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
1141 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
1201 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
1261 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
1321 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
1381 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
1441 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
1501 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
1561 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
1621 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
1681 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
1741 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
1801 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
1861 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
1921 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
1981 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
2041 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
2101 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
2161 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
2221 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
2281 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
2341 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
2401 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
2461 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
2521 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
2581 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
2641 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
2701 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
2761 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
2821 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
2881 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
2941 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
3001 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
3061 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
3121 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
3181 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
3241 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
3301 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
3361 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
3421 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
3481 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
3541 tccccgcgcg ttggccgatt cattaatgca g
//
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