pK7GWIWG2(I)

LOCUS       pK7GWIWG2(I)               13599 bp DNA     circular SYN 13-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13599)
  AUTHORS   Biofeng
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2023 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..13599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    263..387
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        412..442
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             496..1155
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1497..1802
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1843..1967)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    2693..2817
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2858..3163)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(3505..4164)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(4218..4248)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(4273..4397)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4506..4850)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    5543..5567
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6867..7496
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7930..8997
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      9063..9257
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9601..9741
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9927..10515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10759..11550)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    12078..12102
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(12195..12447)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12487..13281)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(13315..13494)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 ctagagggcc cgacgtcgca tgcctgcagg tcactggatt ttggttttag gaattagaaa
       61 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
      121 acacatgagc gaaaccctat aagaacccta attcccttat ctgggaacta ctcacacatt
      181 attctggaga aaaatagaga gagatagatt tgtagagaga gactggtgat ttttgcggac
      241 tctagcatgg ccgcgggata tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      301 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      361 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      421 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccggcgag attttcagga
      481 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      541 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      601 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      661 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      721 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      781 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      841 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      901 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      961 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     1021 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc
     1081 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     1141 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1201 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1261 aaaagaggtg tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1321 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1381 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1441 cgcccggttt attgaaatga acggctcttt tgctgacgag aacagggact ggtgaaatgc
     1501 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1561 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1621 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1681 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1741 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1801 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1861 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1921 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat atcactagtg
     1981 cggccgcctg caggtcgacc atatggtcga cctgcaggcg gccgcactag tgatgctgtt
     2041 atgttcagtg tcaagctgac ctgcaaacac gttaaatgct aagaagttag aatatatgag
     2101 acacgttaac tggtatatga ataagctgta aataaccgag tataaactca ttaactaata
     2161 tcacctctag agtataatat aatcaaattc gacaatttga ctttcaagag taggctaatg
     2221 taaaatcttt atatatttct acaatgttca aagaaacagt tgcatctaaa cccctatggc
     2281 catcaaattc aatgaacgct aagcttaata tgactctcaa taaagtctca taccaacaag
     2341 tgccacctta ttcaaccatc aagaaaaaag ccaaaattta tgctactcta aggaaaactt
     2401 cactaaagaa gacgatttag agtgttttac caagaatttc tgtcatctta ctaaacaact
     2461 aaagatcggt gtgatacaaa acctaatctc attaaagttt atgctaaaat aagcataatt
     2521 ttacccacta agcgtgacca gataaacata actcagcaca ccagagcata tatattggtg
     2581 gctcaaatca tagaaactta cagtgaagac acagaaagcc gtaagaagag gcaagagtat
     2641 gaaaccttac ctcatcattt ccatgaggtt gcttctgatc ccgcgggata tcaccacttt
     2701 gtacaagaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
     2761 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatggtc
     2821 gacctgcaga ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt
     2881 taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa
     2941 cggagaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat
     3001 gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg
     3061 ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac
     3121 gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca gtccctgttc
     3181 tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga
     3241 ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt
     3301 accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa
     3361 ctgtcactgt aatacgctgc ttcatagcac acctcttttt gacatacttc gggtatacat
     3421 atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc
     3481 ttttagtaag ccggatccac gcgtttacgc cccgccctgc cactcatcgc agtactgttg
     3541 taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat
     3601 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     3661 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     3721 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     3781 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     3841 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     3901 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
     3961 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     4021 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     4081 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     4141 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgccggatc
     4201 ctaactcaaa atccacacat tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     4261 taatgcggcc gccatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     4321 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
     4381 tttttgtaca aacttgtgat atcactagtg cggccgcctg caggtcgact agaatagtaa
     4441 attgtaatgt tgtttgttgt ttgttttgtt gtggtaattg ttgtaaaaat acggatcgtc
     4501 ctgcagtcct ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat
     4561 agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg
     4621 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
     4681 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
     4741 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
     4801 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
     4861 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
     4921 tgttgacgaa gattttcttc ttgtcattga gtcgtaaaag actctgtatg aactgttcgc
     4981 cagtcttcac ggcgagttct gttagatcct cgatctgaat ttttgactcc atggcctttg
     5041 attcagtagg aactactttc ttagagactc caatctctat tacttgcctt ggtttatgaa
     5101 gcaagccttg aatcgtccat actggaatag tacttctgat cttgagaaat atatctttct
     5161 ctgtgttctt gatgcagtta gtcctgaatc ttttgactgc atctttaacc ttcttgggaa
     5221 ggtatttgat ctcctggaga ttattactcg ggtagatcgt cttgatgaga cctgccgcgt
     5281 aggcctctct aaccatctgt gggtcagcat tctttctgaa attgaagagg ctaatcttct
     5341 cattatcggt ggtgaacatg gtatcgtcac cttctccgtc gaactttctt cctagatcgt
     5401 agagatagag aaagtcgtcc atggtgatct ccggggcaaa ggagatcagc ttggctctag
     5461 tcgaccatat gggagagctc aagcttagct tgagcttgga tcagattgtc gtttcccgcc
     5521 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
     5581 gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
     5641 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct
     5701 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat
     5761 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
     5821 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
     5881 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
     5941 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
     6001 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
     6061 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
     6121 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
     6181 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
     6241 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
     6301 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
     6361 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
     6421 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
     6481 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
     6541 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
     6601 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
     6661 gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
     6721 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
     6781 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta
     6841 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
     6901 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg
     6961 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
     7021 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
     7081 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
     7141 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
     7201 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
     7261 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
     7321 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
     7381 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
     7441 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
     7501 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
     7561 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
     7621 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
     7681 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa
     7741 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
     7801 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
     7861 taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat
     7921 cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac
     7981 ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca
     8041 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg
     8101 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt
     8161 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc
     8221 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca
     8281 gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac
     8341 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag
     8401 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg
     8461 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc
     8521 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc
     8581 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag
     8641 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg
     8701 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc
     8761 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc
     8821 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg
     8881 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg
     8941 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt
     9001 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt
     9061 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
     9121 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
     9181 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
     9241 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
     9301 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
     9361 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg
     9421 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga
     9481 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     9541 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     9601 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     9661 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     9721 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9781 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9841 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    10381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10561 gaaaactcac gttaagggat tttggtcatg catgatatat ctcccaattt gtgtagggct
    10621 tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg ctgtgagcaa
    10681 ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg cgaagcggcg tcggcttgaa
    10741 cgaatttcta gctagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
    10801 gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
    10861 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
    10921 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
    10981 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
    11041 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
    11101 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
    11161 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
    11221 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
    11281 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
    11341 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
    11401 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
    11461 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
    11521 cgatacttcg gcgatcaccg cttcccccat gatgtttaac tttgttttag ggcgactgcc
    11581 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
    11641 ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacatgtca taacaagaag
    11701 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
    11761 tgcgtgacgg cagttacgct acttgcatta cagcttacga accgaacgag gcttatgtcc
    11821 actgggttcg tgcccgaatt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca
    11881 tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg
    11941 aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg
    12001 tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
    12061 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
    12121 cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt gaattatcag
    12181 cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt
    12241 tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat
    12301 aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta
    12361 attcaacaga aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa
    12421 actttattgc caaatgtttg aacgatctgc ttgactctag ctagagtccg aaccccagag
    12481 tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
    12541 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
    12601 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
    12661 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccgtg
    12721 ggtcacgacg agatcctcgc cgtcgggcat ccgcgccttg agcctggcga acagttcggc
    12781 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
    12841 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
    12901 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
    12961 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
    13021 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
    13081 tagccgcgct gcctcgtctt ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa
    13141 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
    13201 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
    13261 caatccatct tgttcaatca tgcctcgatc gagttgagag tgaatatgag actctaattg
    13321 gataccgagg ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa tatttgctag
    13381 ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta
    13441 gctcattaaa ctccagaaac ccgcggctga gtggctcctt caacgttgcg gttctgtcag
    13501 ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta
    13561 attctcatgt atgataattc gagggtaccc ggggatcct
//