LOCUS ARR3tk-eGFP/SV40-mCherry 8622 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION To measure transcriptional activity of Androgen Receptor.
ACCESSION .
VERSION .
KEYWORDS ARR3tk-eGFP/SV40-mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8622)
AUTHORS Lee E, Wongvipat J, Choi D, Wang P, Lee YS, Zheng D, Watson PA,
Gopalan A, Sawyers CL
TITLE GREB1 amplifies androgen receptor output in human prostate cancer
and contributes to antiandrogen resistance.
JOURNAL Elife. 2019 Jan 15;8. pii: 41913. doi: 10.7554/eLife.41913.
PUBMED 30644358
REFERENCE 2 (bases 1 to 8622)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8622
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 98..115
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 232..253
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 268..298
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 306..322
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 311..333
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 330..346
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 330..346
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 365..385
/label=T3
/note="T3 promoter, forward primer"
promoter 367..385
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 413..639
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 640..820
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 867..992
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1485..1718
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1903..1947
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2214..2331
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2888..2919
/label=minP
/note="minimal TATA-box promoter with low basal activity"
regulatory 2975..2984
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2981..3700
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 2981..3697
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3026..3047)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3287..3306)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3634..3655
/label=EGFP-C
/note="EGFP, forward primer"
promoter 3794..4151
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3817..3837)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 4002..4137
/label=SV40 ori
/note="SV40 origin of replication"
CDS 4161..4871
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(4306..4324)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 4574..4593
/label=mCherry-F
/note="mCherry, forward primer"
misc_feature 4878..5466
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4931..4951)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5349..5360)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5553..5786
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5858..5979
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5895..5914)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5949..5968
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6019..6154
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(6174..6193)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6175..6193)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6203..6220)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6203..6219)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6212..6234)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6361..6816
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6448..6467)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6658..6679
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6842..6946
/gene="bla"
/label=AmpR promoter
CDS 6947..7807
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7165..7184)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7978..8566
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8467..8486
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
421 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
481 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
541 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
601 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
841 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
901 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
961 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
1021 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
1081 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
1141 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
1201 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
1261 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
1321 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
1381 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
1441 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
1501 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
1561 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
1621 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
1681 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
1741 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
1801 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
1861 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
1921 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
1981 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
2041 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
2101 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
2161 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
2221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
2281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatggatcca
2341 agctcagatc caagcttgga gcttatgata gcatcttgtt cttagtcttt ttcttaatag
2401 ggacataaag cccacaaata aaaatatgcc tgaagaatgg gacaggcatt gggcattgtc
2461 catgcctagt aaagtactcc aagaacctat ttgtatacta gatgacacaa tgttctagcc
2521 aagcttggag cttatgatag catcttgttc ttagtctttt tcttaatagg gacataaagc
2581 ccacaaataa aaatatgcct gaagaatggg acaggcattg ggcattgtcc atgcctagta
2641 aagtactcca agaacctatt tgtatactag atgacacaat gttctagcca agcttatgat
2701 agcatcttgt tcttagtctt tttcttaata gggacataaa gcccacaaat aaaaatatgc
2761 ctgaagaatg ggacaggcat tgggcattgt ccatgcctag taaagtactc caagaaccta
2821 tttgtatact agatgacaca atgttctagg ggtaccgagc tcgagcccgg gctcgagatc
2881 tagactctag agggtatata atggaagctc gaattccagc ttggcattcc ggtactgttg
2941 gtaaaaagct tggcattccg gtactgttgg taaagccacc atggtgagca agggcgagga
3001 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
3061 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
3121 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
3181 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
3241 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
3301 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
3361 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
3421 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
3481 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
3541 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
3601 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
3661 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg actctagagt
3721 cgagaagctt gatgatctgc gcagcaccat ggcctgaaat aacctctgaa agaggaactt
3781 ggttaggtac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
3841 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
3901 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
3961 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
4021 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
4081 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
4141 gcttttgcaa aaagcttacc atggtgagca agggcgagga ggataacatg gccatcatca
4201 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
4261 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
4321 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
4381 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
4441 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
4501 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
4561 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
4621 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
4681 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
4741 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
4801 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
4861 tgtacaagta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
4921 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
4981 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
5041 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
5101 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
5161 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
5221 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
5281 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
5341 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
5401 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
5461 ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
5521 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
5581 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
5641 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
5701 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
5761 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
5821 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
5881 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
5941 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
6001 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
6061 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
6121 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
6181 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
6241 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
6301 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
6361 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
6421 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
6481 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
6541 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
6601 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
6661 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
6721 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
6781 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
6841 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
6901 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
6961 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
7021 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
7081 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
7141 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
7201 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
7261 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
7321 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
7381 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
7441 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
7501 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
7561 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
7621 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
7681 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
7741 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
7801 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
7861 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
7921 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
7981 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
8041 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
8101 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
8161 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
8221 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
8281 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
8341 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
8401 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
8461 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
8521 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
8581 ggccttttta cggttcctgg ccttttgctg gccttttgct ca
//
