LOCUS HA-HA-AR-NTD 7070 bp ds-DNA circular SYN 25-JUN-2021
DEFINITION Mammalian expression of 2xHA-tagged human androgen receptor
trancate: AR N-terminal-domain (NTD).
ACCESSION .
VERSION .
KEYWORDS HA-HA-AR-NTD
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7070)
AUTHORS Liu X, Han W, Gulla S, Simon NI, Gao Y, Cai C, Yang H, Zhang X, Liu
J, Balk SP, Chen S
TITLE Protein phosphatase 1 suppresses androgen receptor ubiquitylation
and degradation.
JOURNAL Oncotarget. 2016 Jan 12;7(2):1754-64. doi: 10.18632/oncotarget.6434.
PUBMED 26636645
REFERENCE 2 (bases 1 to 7070)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 25, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7070
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 928..954
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 970..996
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(2661..2678)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2667..2891
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2937..3365
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3024..3043)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3234..3255
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3374..3394)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3379..3708
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3559..3694
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3621..3640
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3775..4569
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3829..3848)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4439..4458
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 4743..4864
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4780..4799)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4834..4853
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4913..4929)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4913..4929)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4926..4948)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4937..4953
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4961..4991)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5006..5027
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5144..5161)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5315..5903)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5395..5414)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6074..6934)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6697..6716
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6935..7039)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttccac catggcctac ccatacgacg tcccagacta cgctcagctc
961 cagcttatgt acccctacga cgtgcccgac tacgcggatc caatggaagt gcagttaggg
1021 ctgggaaggg tctaccctcg gccgccgtcc aagacctacc gaggagcttt ccagaatctg
1081 ttccagagcg tgcgcgaagt gatccagaac ccgggcccca ggcacccaga ggccgcgagc
1141 gcagcacctc ccggcgccag tttgctgctg ctgcagcagc agcagcagca gcagcagcag
1201 cagcagcagc agcagcagca gcagcagcag cagcagcaag agactagccc caggcagcag
1261 cagcagcagc agggtgagga tggttctccc caagcccatc gtagaggccc cacaggctac
1321 ctggtcctgg atgaggaaca gcaaccttca cagccgcagt cggccctgga gtgccacccc
1381 gagagaggtt gcgtcccaga gcctggagcc gccgtggccg ccagcaaggg gctgccgcag
1441 cagctgccag cacctccgga cgaggatgac tcagctgccc catccacgtt gtccctgctg
1501 ggccccactt tccccggctt aagcagctgc tccgctgacc ttaaagacat cctgagcgag
1561 gccagcacca tgcaactcct tcagcaacag cagcaggaag cagtatccga aggcagcagc
1621 agcgggagag cgagggaggc ctcgggggct cccacttcct ccaaggacaa ttacttaggg
1681 ggcacttcga ccatttctga caacgccaag gagttgtgta aggcagtgtc ggtgtccatg
1741 ggcctgggtg tggaggcgtt ggagcatctg agtccagggg aacagcttcg gggggattgc
1801 atgtacgccc cacttttggg agttccaccc gctgtgcgtc ccactccttg tgccccattg
1861 gccgaatgca aaggttctct gctagacgac agcgcaggca agagcactga agatactgct
1921 gagtattccc ctttcaaggg aggttacacc aaagggctag aaggcgagag cctaggctgc
1981 tctggcagcg ctgcagcagg gagctccggg acacttgaac tgccgtctac cctgtctctc
2041 tacaagtccg gagcactgga cgaggcagct gcgtaccaga gtcgcgacta ctacaacttt
2101 ccactggctc tggccggacc gccgccccct ccgccgcctc cccatcccca cgctcgcatc
2161 aagctggaga acccgctgga ctacggcagc gcctgggcgg ctgcggcggc gcagtgccgc
2221 tatggggacc tggcgagcct gcatggcgcg ggtgcagcgg gacccggttc tgggtcaccc
2281 tcagccgccg cttcctcatc ctggcacact ctcttcacag ccgaagaagg ccagttgtat
2341 ggaccgtgtg gtggtggtgg gggtggtggc ggcggcggcg gcggcggcgg cggcggcggc
2401 ggcggcggcg gcggcggcga ggcgggagct gtagccccct acggctacac tcggccccct
2461 caggggctgg cgggccagga aagcgacttc accgcacctg atgtgtggta ccctggcggc
2521 atggtgagca gagtgcccta tcccagtccc acttgtgtca aaagcgaaat gggcccctgg
2581 atggatagct actccggacc ttacggggac atgcgtgcgg ccgctcgagt ctagagggcc
2641 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
2701 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
2761 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
2821 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
2881 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
2941 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
3001 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
3061 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
3121 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
3181 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
3241 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
3301 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
3361 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
3421 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
3481 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
3541 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
3601 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
3661 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
3721 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
3781 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
3841 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
3901 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
3961 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
4021 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
4081 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
4141 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
4201 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
4261 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
4321 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
4381 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
4441 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
4501 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
4561 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
4621 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
4681 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
4741 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
4801 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
4861 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
4921 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
4981 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
5041 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
5101 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
5161 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
5221 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
5281 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
5341 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
5401 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
5461 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
5521 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
5581 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
5641 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
5701 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
5761 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
5821 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
5881 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
5941 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
6001 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
6061 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
6121 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
6181 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
6241 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
6301 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
6361 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
6421 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
6481 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
6541 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
6601 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
6661 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
6721 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
6781 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
6841 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
6901 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
6961 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
7021 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
