| 质粒名称: | MOS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 64120 |
| 存储实验室: | Linzhao Cheng |
|
载体骨架基本信息 |
|
| 载体名称: | pCEP4 |
| 载体抗性: | 氨苄青霉素 |
| 完整质粒大小: | 10503 bp |
| 载体类型: | 哺乳细胞表达 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | OCT4-2A-SOX2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2091 bp |
| 启动子 | SFFV |
| 标签/融合蛋白: |
Myc（插入的 C 端子）
HA（插入式 C 端子）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AsiSI（销毁后未知） |
| 3' 克隆位点: | PmeI（销毁后未知） |
| 5' 测序引物 | 5-AGGCATGGAAAAATACCAAA, or 5-TAACCAATCAGCCTGCTTCT |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | MOS was a gift from Linzhao Cheng (Addgene plasmid # 64120 ; http://n2t.net/addgene:64120 ; RRID:Addgene_64120) |
| 参考文献部分: | A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Chou BK, Gu H, Gao Y, Dowey SN, Wang Y, Shi J, Li Y, Ye Z, Cheng T, Cheng L. Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214. 10.5966/sctm.2014-0214 PubMed 25742692 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS MOS 9879 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION A modified episomal (EBNA1/oriP) vector expressing human OCT4 and
SOX2 genes.
ACCESSION .
VERSION .
KEYWORDS MOS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9879)
AUTHORS Chou BK, Gu H, Gao Y, Dowey SN, Wang Y, Shi J, Li Y, Ye Z, Cheng T,
Cheng L
TITLE A Facile Method to Establish Human Induced Pluripotent Stem Cells
From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture
Conditions: A Clinically Compliant Approach.
JOURNAL Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214.
PUBMED 25742692
REFERENCE 2 (bases 1 to 9879)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9879
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 120..527
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
CDS 1621..1680
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/label=E2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="QCTNYALLKLAGDVESNPGP"
CDS 2650..2679
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 2698..2721
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
primer_bind complement(2791..2811)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3209..3220)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3317..3451
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3341..3360)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3395..3414
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(3594..3616)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 4179..5968
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
primer_bind complement(8006..8024)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 8092..8196
/gene="bla"
/label=AmpR promoter
CDS 8197..9057
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8415..8434)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9228..9816
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9717..9736
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
61 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgag ctagctgcag
121 taacgccatt ttgcaaggca tggaaaaata ccaaaccaag aatagagaag ttcagatcaa
181 gggcgggtac atgaaaatag ctaacgttgg gccaaacagg atatctgcgg tgagcagttt
241 cggccccggc ccggggccaa gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc
301 aagaacagat ggtccccaga tatggcccaa ccctcagcag tttcttaaga cccatcagat
361 gtttccaggc tcccccaagg acctgaaatg accctgcgcc ttatttgaat taaccaatca
421 gcctgcttct cgcttctgtt cgcgcgcttc tgcttcccga gctctataaa agagctcaca
481 acccctcact cggcgcgcca gtcctccgac agactgagtc gcccgggtac cgcgatcgcc
541 atggcgggac acctggcttc ggatttcgcc ttctcgcccc ctccaggtgg tggaggtgat
601 gggccagggg ggccggagcc gggctgggtt gatcctcgga cctggctaag cttccaaggc
661 cctcctggag ggccaggaat cgggccgggg gttgggccag gctctgaggt gtgggggatt
721 cccccatgcc ccccgccgta tgagttctgt ggggggatgg cgtactgtgg gccccaggtt
781 ggagtggggc tagtgcccca aggcggcttg gagacctctc agcctgaggg tgaagcagga
841 gtcggggtgg agagcaactc cgatggggcc tccccggagc cctgcaccgt cacccctggt
901 gccgtgaagc tggagaagga gaagctggag caaaacccgg aggagtccca ggacatcaaa
961 gctctgcaga aagaactcga gcaatttgcc aagctcctga agcagaagag gatcaccctg
1021 ggatatacac aggccgatgt ggggctcacc ctgggggttc tatttgggaa ggtattcagc
1081 caaacgacca tctgccgctt tgaggctctg cagcttagct tcaagaacat gtgtaagctg
1141 cggcccttgc tgcagaagtg ggtggaggaa gctgacaaca atgaaaatct tcaggagata
1201 tgcaaagcag aaaccctcgt gcaggcccga aagagaaagc gaaccagtat cgagaaccga
1261 gtgagaggca acctggagaa tttgttcctg cagtgcccga aacccacact gcagcagatc
1321 agccacatcg cccagcagct tgggctcgag aaggatgtgg tccgagtgtg gttctgtaac
1381 cggcgccaga agggcaagcg atcaagcagc gactatgcac aacgagagga ttttgaggct
1441 gctgggtctc ctttctcagg gggaccagtg tcctttcctc tggccccagg gccccatttt
1501 ggtaccccag gctatgggag ccctcacttc actgcactgt actcctcggt ccctttccct
1561 gagggggaag cctttccccc tgtctccgtc accactctgg gctctcccat gcattcaaac
1621 cagtgtacta attatgctct cttgaaattg gctggagatg ttgagagcaa cccaggtccc
1681 atgtacaaca tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc
1741 ggcggcggca actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc
1801 gtcaagcggc ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc
1861 caggagaacc ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa
1921 cttttgtcgg agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg
1981 cacatgaagg agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg
2041 aagaaggata agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg
2101 agcggggtcg gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac
2161 gcgcacatga acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac
2221 ccgcagcacc cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac
2281 gacgtgagcg ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg
2341 cccacctaca gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg
2401 ggttcggtgg tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac
2461 tccagggcgc cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc
2521 gccgaggtgc cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc
2581 ggcccggtgc ccggcacggc cattaacggc acactgcccc tctcacacat gacgcgtacg
2641 cggccgctcg agcagaaact catctcagaa gaggatctgg cagcaaatga tatcctggat
2701 tacaaggatg acgacgataa ggtttaaacg cgtcgacaat caacctctgg attacaaaat
2761 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
2821 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
2881 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
2941 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
3001 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
3061 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
3121 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
3181 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
3241 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
3301 gatctccctt tgggcggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
3361 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
3421 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
3481 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
3541 tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
3601 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
3661 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gaggtcgact
3721 ctactagagg atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc
3781 ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc
3841 cctgttccac atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg
3901 acatccttat aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag
3961 actttgcagt ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc
4021 tcttacacca atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct
4081 acaccaacgt caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca
4141 ttagcaatag tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg
4201 ggtagtagta tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga
4261 agcatatgct atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac
4321 cccatgagct gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca
4381 ttttagtcac aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct
4441 tcgcctgctt cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag
4501 taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt
4561 ggacgggggg ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct
4621 tgggcaataa atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc
4681 atggggtggg gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg
4741 gcaacacata atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg
4801 accaagacag gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac
4861 gccgacagca gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc
4921 acgccaatgg ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt
4981 gggggcacgc gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt
5041 aataacttgg ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac
5101 cactaatggc accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc
5161 gattgctgcg atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt
5221 tgttggtcct catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta
5281 gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat
5341 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
5401 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
5461 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
5521 atgctatcct aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat
5581 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc
5641 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
5701 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
5761 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
5821 tatcctaatc tgtatccggg tagcatatgc tatcctcatg catatacagt cagcatatga
5881 tacccagtag tagagtggga gtgctatcct ttgcatatgc cgccacctcc caagggggcg
5941 tgaattttcg ctgcttgtcc ttttcctgct ggttgctccc attcttaggt gaatttaagg
6001 aggccaggct aaagccgtcg catgtctgat tgctcaccag gtaaatgtcg ctaatgtttt
6061 ccaacgcgag aaggtgttga gcgcggagct gagtgacgtg acaacatggg tatgcccaat
6121 tgccccatgt tgggaggacg aaaatggtga caagacagat ggccagaaat acaccaacag
6181 cacgcatgat gtctactggg gatttattct ttagtgcggg ggaatacacg gcttttaata
6241 cgattgaggg cgtctcctaa caagttacat cactcctgcc cttcctcacc ctcatctcca
6301 tcacctcctt catctccgtc atctccgtca tcaccctccg cggcagcccc ttccaccata
6361 ggtggaaacc agggaggcaa atctactcca tcgtcaaagc tgcacacagt caccctgata
6421 ttgcaggtag gagcgggctt tgtcataaca aggtccttaa tcgcatcctt caaaacctca
6481 gcaaatatat gagtttgtaa aaagaccatg aaataacaga caatggactc ccttagcggg
6541 ccaggttgtg ggccgggtcc aggggccatt ccaaagggga gacgactcaa tggtgtaaga
6601 cgacattgtg gaatagcaag ggcagttcct cgccttaggt tgtaaaggga ggtcttacta
6661 cctccatata cgaacacacc ggcgacccaa gttccttcgt cggtagtcct ttctacgtga
6721 ctcctagcca ggagagctct taaaccttct gcaatgttct caaatttcgg gttggaacct
6781 ccttgaccac gatgctttcc aaaccaccct ccttttttgc gcctgcctcc atcaccctga
6841 ccccggggtc cagtgcttgg gccttctcct gggtcatctg cggggccctg ctctatcgct
6901 cccgggggca cgtcaggctc accatctggg ccaccttctt ggtggtattc aaaataatcg
6961 gcttccccta cagggtggaa aaatggcctt ctacctggag ggggcctgcg cggtggagac
7021 ccggatgatg atgactgact actgggactc ctgggcctct tttctccacg tccacgacct
7081 ctccccctgg ctctttcacg acttcccccc ctggctcttt cacgtcctct accccggcgg
7141 cctccactac ctcctcgacc ccggcctcca ctacctcctc gaccccggcc tccactgcct
7201 cctcgacccc ggcctccacc tcctgctcct gcccctcctg ctcctgcccc tcctcctgct
7261 cctgcccctc ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc accgtgggtc
7321 cctttgcagc caatgcaact tggacgtttt tggggtctcc ggacaccatc tctatgtctt
7381 ggccctgatc ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt
7441 ccccgtcctc gtccatggtt atcaccccct cttctttgag gtccactgcc gccggagcct
7501 tctggtccag atgtgtctcc cttctctcct aggccatttc caggtcctgt acctggcccc
7561 tcgtcagaca tgattcacac taaaagagat caatagacat ctttattaga cgacgctcag
7621 tgaatacagg gagtgcagac tcctgccccc tccaacagcc cccccaccct catccccttc
7681 atggtcgctg tcagacagat ccaggtctga aaattcccca tcctccgaac catcctcgtc
7741 ctcatcacca attactcgca gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc
7801 ctgagcctca agccaggcct caaattcctc gtcccccttt ttgctggacg gtagggatgg
7861 ggattctcgg gacccctcct cttcctcttc aaggtcacca gacagagatg ctactggggc
7921 aacggaagaa aagctgggtg cggcctgtga ggatcagctt atcgatgata agctgtcaaa
7981 catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
8041 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
8101 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
8161 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
8221 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
8281 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
8341 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
8401 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
8461 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
8521 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
8581 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
8641 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
8701 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
8761 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
8821 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
8881 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
8941 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
9001 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
9061 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
9121 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
9181 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
9241 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
9301 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
9361 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
9421 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
9481 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
9541 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
9601 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
9661 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
9721 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
9781 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
9841 cggttcctgg ccttttgctg gccttgaagc tgtccctga
//
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