| 出品公司: | Origene |
|---|---|
| 载体名称: | pEX-N-His-GST |
| 质粒类型: | 大肠杆菌表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | T7启动子 |
| 载体大小: | 5.2 kb |
| 5’测序引物: | pGEX 5' GGGCTGGCAAGCCACGTTTGGTG |
| 3’测序引物: | T7t: GCTAGTTATTGCTCAGCGG |
| 载体标签: | N-His，N-GST |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5 alpha |
| 宿主细胞（系）: | BL21(DE3) |
| 产品目录号: | PS100028 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
| 备注: | 大肠杆菌表达载体。 |
买家导航
pEX-N-His-GST载体质粒基本信息
pEX-N-His-GST质粒图谱载体图谱和pEX-N-His-GST载体序列质粒序列多克隆位点信息
pEX-N-His-GST质粒载体简介
pEX-N-His-GST质粒序列载体序列
LOCUS pEX-N-His-GST 5227 bp DNA circular SYN 12-NOV-2024
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5227)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Nov 12, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
https://www.biofeng.com
COMMENT VNTDATE|1073779200|
COMMENT VNTDBDATE|1073779200|
COMMENT VNTNAME|pEX-N-His-GST|
COMMENT VNTAUTHORNAME|Ming|
FEATURES Location/Qualifiers
primer_bind 155..171
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 207..225
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 226..250
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 254..276
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 287..304
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
CDS 311..964
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
CDS 986..1006
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
terminator 1132..1178
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 1424..1501
/gene="lacI"
/label=lacI promoter
CDS 1502..2584
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter 2633..2663
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2671..2687
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2695..2711
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2727..2743)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(3035..3623)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3794..4654)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
promoter complement(4655..4759)
/gene="bla"
/label=AmpR promoter
rep_origin 4786..5214
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
61 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
121 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccgaattc
181 tgctggactt tccaaaatgt cgagcttaat acgactcact ataggggaat tgtgagcgga
241 taacaattcc ccttttgttt aactttaaga aggagaggcc accatgcatc accatcacca
301 tcacgcaggc atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac
361 tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga
421 aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta
481 ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga
541 caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg
601 agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac
661 tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt
721 atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga
781 cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt
841 ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa
901 gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc
961 aaaatcggat atcatcccaa cgaccgaaaa cctgtatttt cagggcgcga tcgccggcgc
1021 gccagatctc aagcttaact agctagcgga ccgacgcgtt aaacgcggcc gctcgaggtt
1081 taaacggccg gccacatgac tgactgagcc acgataaccg ctgagcaata actagcataa
1141 ccccttgggg cctctaaacg ggtcttgagg ggttttttcc caccagcctt gtcctaatcc
1201 gcggcggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
1261 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
1321 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
1381 tctccttacg catctgtgcg gtatttcaca ccgcataaat tccgacacca tcgaatggcg
1441 caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa
1501 tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
1561 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
1621 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
1681 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
1741 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
1801 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgagtcag
1861 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
1921 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
1981 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
2041 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
2101 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
2161 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
2221 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
2281 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
2341 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
2401 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
2461 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
2521 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
2581 gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccccag gctttacact
2641 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
2701 acagctatga ccatgattac ggattcactg gccgtcgttt tacaacgtcg tgactgggaa
2761 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
2821 aatagcgaag aggcccgcac cgatggatcc gctcttccgc ttcctcgctc actgactcgc
2881 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
2941 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
3001 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
3061 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
3121 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
3181 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
3241 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
3301 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
3361 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
3421 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
3481 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
3541 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
3601 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
3661 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
3721 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
3781 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
3841 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
3901 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
3961 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
4021 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
4081 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
4141 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
4201 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
4261 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
4321 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
4381 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
4441 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
4501 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
4561 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
4621 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
4681 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
4741 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
4801 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
4861 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
4921 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
4981 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
5041 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
5101 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
5161 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
5221 aaatatt
//
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