| 质粒名称: | pDL278_P23-DsRed-Express2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 121505 |
| 存储实验室: | Vladislav Verkhusha |
|
载体骨架基本信息 |
|
| 载体名称: | pDL278 |
| 空载体大小: | 6700 bp |
| 完整质粒大小: | 7462 bp |
| 载体类型: |
E. coli streptococcal shuttle vector
Growth in Bacteria
|
| 载体抗性: | Spectinomycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | DsRed-Express2 |
| 物种来源: | Synthetic |
| 插入大小: | 830 bp |
| GenBank编号 | MK301207 |
| 启动子: | P23 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SacI (unknown if destroyed) |
| 3’克隆位点: | SphI (unknown if destroyed) |
| 5’测序引物 | M13_pUC_rev |
| 3’测序引物 |
M13_pUC_fwd; pBAD_rev
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: |
pDL278_P23-DsRed-Express2 was a gift from Robert Shields (Addgene plasmid # 121505 ; http://n2t.net/addgene:121505 ; RRID:Addgene_121505)
|
| 参考文献部分: | Fluorescence tools adapted for real-time monitoring of the behaviors of Streptococcus species. Shields RC, Kaspar JR, Lee K, Underhill SAM, Burne RA. Appl Environ Microbiol. 2019 May 17. pii: AEM.00620-19. doi: 10.1128/AEM.00620-19. 10.1128/AEM.00620-19 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDL278_P23-DsRed-Express2 7462 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION E. coli-streptococcal shuttle vector that expresses rfp.
ACCESSION .
VERSION .
KEYWORDS pDL278_P23-DsRed-Express2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7462)
AUTHORS Shields RC, Kaspar JR, Lee K, Underhill SAM, Burne RA
TITLE Fluorescence tools adapted for real-time monitoring of the behaviors
of Streptococcus species.
JOURNAL Appl Environ Microbiol. 2019 May 17. pii: AEM.00620-19. doi:
10.1128/AEM.00620-19.
PUBMED 31101614
REFERENCE 2 (bases 1 to 7462)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7462
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..31
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 39..55
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 44..66
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 63..79
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 63..79
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 244..921
/codon_start=1
/product="noncytotoxic tetrameric variant of DsRed
fluorescent protein (Strack et al., 2008)"
/label=DsRed-Express2
/note="mammalian codon-optimized"
/translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
LFQ"
primer_bind complement(424..444)
/label=DsRed1-N
/note="DsRed1, reverse primer"
primer_bind 642..661
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 836..859
/label=DsRed1-C
/note="DsRed1, forward primer"
primer_bind complement(989..1006)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(989..1005)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(998..1020)
/label=M13/pUC Forward
/note="In lacZ gene"
terminator 1479..1565
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1657..1684
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 1977..2063
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2155..2182
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
rep_origin 6401..6989
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6890..6909
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7143..7160
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 7427..7448
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
61 cacaggaaac agctatgacc atgattacgc caagctttcg aaaagccctg acaaccctcg
121 ttcctaaaaa ggaataagcg tttggtcagt aaataataga aataaaaaat cagacctaag
181 actgatgaca aaaagagcaa attttgataa aatagtatta attactagtt aaggaggaca
241 aacatggata gcactgagaa cgtcatcaag cccttcatgc gcttcaaggt gcacatggag
301 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggcaa gccctacgag
361 ggcacccaga ccgccaagct gcaggtgacc aagggcggcc ccctgccctt cgcctgggac
421 atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc
481 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
541 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg caccttcatc
601 taccacgtga agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag
661 actctgggct gggagccctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
721 gagatccaca aggcgctgaa gctgaagggc ggcggccact acctggtgga gttcaagtca
781 atctacatgg ccaagaagcc cgtgaagctg cccggctact actacgtgga ctccaagctg
841 gacatcacct cccacaacga ggactacacc gtggtggagc agtacgagcg cgccgaggcc
901 cgccaccacc tgttccagta gctcggtacc aaattccaga aaagaggcct cccgaaaggg
961 gcgccttttt tcgttttggt ccgaattcac tggccgtcgt tttacaacgt cgtgactggg
1021 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
1081 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
1141 aatgccggga attattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
1201 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
1261 gcacgcggcg catctcggct gttttggcgg atgagagaag attttcagcc tgatacagat
1321 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
1381 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
1441 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
1501 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
1561 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
1621 gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc
1681 tttttgcgtt tctacaaact cttcctgtcg tcatatctac aagccatccc cccacagata
1741 cggtaaacta gcctcgtttt tgcatcagga aagcagctgt tttggcggat gagagaagat
1801 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
1861 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
1921 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
1981 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
2041 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
2101 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
2161 ccatcctgac ggatggcctt tttgcgtttc tacaaactct tcctgtcgtc atatctacaa
2221 gccatccccc cacagatacg gtaaactagc ctcgtttttg catcaggaaa gcagtcgggc
2281 agcgttggga ttctgagacc tctgagaggc tctcagagct atctaaagct gagggatata
2341 taaatacctt agaaaattat tcgaagagct tagaagcgaa aatagagcgt ttagagcgcg
2401 aggggctgaa attagaaaaa ctaaaaacac aaatagctga cctaaaaatc atgtctgaga
2461 aagaactagc ggctattacc cctaaaaaag gcgtgttcgg taaagaatat gtggaattga
2521 ctaaagagca gtttgaagaa tttaaagggc tgatataccg aagcagaaac cttgttcatc
2581 aaaaagagct agagaatgag caattaaggc ggatagtgcc tctgagacgc tctaaaaacg
2641 gttttgaagc gagtttggaa cgagctaaag aaaaaagtaa gggagagagc atagagcgtc
2701 ttaggaacga aaatagagcg cttagaagtg aaaactcagt tttgagacag caaaatgaca
2761 aaatgctagg aaaactgaaa gagtttatgc cagataaagc cttaaaaaat tttatatcag
2821 agttaagagc tattcagcca atcgtaaggg tagttaaacg agtgattgaa aaagggctag
2881 gcctttgagc gatttatgcc gtgaaagcta attgacaata agcaagggca aagtacgcta
2941 ggacgtgacg agccgaaagg ctttagcgtt tcgagccgac acggacaaag gacgtccgcc
3001 cttggttact tgttgtcaat tagaccatgg aataaagtaa gcggacatgg tataatagct
3061 aggtcgcaac gttctttcgc taagttacga acttagattg gaggtgagcg ctgtgaagac
3121 tttcctagaa cttgttttga taccttttgt ggttggcgtt gctgcagagg taagtgctga
3181 ttggttgatt cggtatgttc gaaacaaacg cgacaaaaat taacctgagt tctttttgag
3241 gacaaaaaag aaagacagta gttccagcta ctgttttttt tgcgttgtgc tattcgtttc
3301 ctagaacttc tagcgttaaa attattatac cacgtttgga tttagaaagt caaaatttga
3361 ggttttaggg gtgaattttt cgtgaaacga aaaagagggc tgaaaagccc ttaaaaaccc
3421 atgcgtagca agggtttttt cttatcttga taaaataaag tttataattt aagtgtccaa
3481 tttaaaataa taaacttagg agaattgcag gaactttttt atatactcaa aaaaattttt
3541 ttgcaagaaa attataacat gacaggtact gaaaatcaag tctttaagga ctattctaag
3601 aatggcaaag atagaaagtg gcgagaacgc aagttaaaaa atattgagct tgctggtcgt
3661 ttagagcgtt taggatatcg ttcgtttgaa cgggtctatc aatgtgccga agtgttgaag
3721 tttatcgaac aacaagacgg cacgaaaaaa ctctatcagt cttatttttg caaaaataag
3781 ctctgcccaa tttgtaactg gagacggtca atgaagtatg cttatcaagc tgaattagtg
3841 gtaaatgaag caatgaaacg ctatccaaaa ggtcgctttc tctttttgac cttgacgatt
3901 aagaacatta gtggtgaaaa attaaataaa tcaatttcag aaatagggcg agcttttaat
3961 cgtcttatga aatacaagaa agtcgataaa aatgttattg gctatttgcg agccactgag
4021 gtaacttatt caactgagca tgagaattat caccctcatt tgcatgtatt gttatttgtg
4081 aaatctagct attttactgg aaataataca aattacatta gccaagaaga atggacgaaa
4141 ctatgggcta aggcgatgaa attggattat acacctgtag ttgatattcg aaccgtcaaa
4201 gctcataaac gtaaaaactt gaagtcagct attatcgaaa cagctaaata tcctgttaag
4261 ccttttgatg tagatacaga agatgtgaca ttattctctg aaatggtcaa agaacggata
4321 acagaagatt taacgaatgg tttgcaccga aaaaggcaga ttggttttgg aaagttgttc
4381 aagaaaatca aggcggagtt agctcttgat gatgtcgaag aagggaatct tgttcagacc
4441 ggagcagaag aatctgcaga aagtactggt cgtgaaattg ttgccttttg gaattgggat
4501 agaaagaatt attttgtgag gtagctgatg agtaaaacag tatcagaatt agctcaagaa
4561 ttgggagtta gtaggcagta tcttaatcgg attttatcgc aaaataatct cggtcgaaaa
4621 aaagggaata aaaaagtagt ttccgatatg gacgagaaag ttatgcaagg gtttattgtt
4681 ttctaaaatc tgattaccaa ttagaatgaa tatttcccaa atattaaata ataaaacaaa
4741 aaaattgaaa aaagtgtttc caccattttt tcaatttttt tataattttt ttaatctgtt
4801 atttaaatag tttatagtta aatttacatt ttcattagtc cattcaatat tctctccaag
4861 ataactacga actgctaaca aaattctctc cctatgttct aatggagaag attcagccac
4921 tgcatttccc gcaatatctt ttggtatgat tttacccgtg tccatagtta aaatcatacg
4981 gcataaagtt aatatagagt tggtttcatc atcctgataa ttatctatta attcctctga
5041 cgaatccata atggctcttc tcacatcaga aaatggaata tcaggtagta attcctctaa
5101 gtcataattt ccgtatattc ttttattttt tcgttttgct tggtaaagca ttatggttaa
5161 atctgaattt aattccttct gaggaatgta tccttgttca taaagctctt gtaaccattc
5221 tccataaata aattcttgtt tgggaggatg attccacggt accatttctt gctgaataat
5281 aattgttaat tcaatatatc gtaagttgct tttatctcct attttttttg aaataggtct
5341 aattttttgt ataagtattt ctttactttg atctgtcaat ggttcagata cgacgactaa
5401 aaagtcaaga tcactatttg gttttagtcc actctcaact cctgatccaa acatgtaagt
5461 accaataagg ttatttttta aatgtttccg aagtattttt ttcactttat taatttgttc
5521 gtatgtattc aaatatatcc tcctcactat tttgattagt acctatttta tatccatagt
5581 tgttaattaa ataaacttaa tttagtttat ttatagattt cattggcttc taaatttttt
5641 atctagataa taattatttt agttaatttt attctagatt atatatgata tgatctttca
5701 tttccataaa actaaagtaa gtgtaaacct attcattgtt ttaaaaatat ctcttgccag
5761 tcacgttacg ttattagtta tagttattat aacatgtatt cacgaacgaa aaatcttcta
5821 tataaaagat atattatctt atcagtattg tcaatatatt caaggcaatc tgcctcctca
5881 tcctcttcat cctcttcgtc ttggtagctt tttaaatatg gcgcttcata gagtaattct
5941 gtaaaggtcc aattctcgtt ttcatacctc ggtataatct tacctatcac ctcaaatggt
6001 tcgctgggtt tatcggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
6061 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
6121 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
6181 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
6241 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
6301 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
6361 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
6421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
6481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
6541 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
6601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
6661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
6721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
6781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
6841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
6901 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
6961 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
7021 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
7081 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
7141 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
7201 cgcgttggcc gattcattaa tgcagaaata cgtttcccat ttagccgtca tttcagcact
7261 cgttaaaagg tgctgacttt caactgcctg gcacaataat tttccttttt cagttacaac
7321 aagctccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
7381 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
7441 tcactcatta ggcaccccag gc
//
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