质粒名称: | pCgVchCAST-crtYf |
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出品公司: | Addgene |
目录编号: | 203816 |
存储实验室: | Sheng Yang |
载体骨架基本信息 |
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载体名称: | pJYS1Ptac |
完整质粒大小: | 14906bp |
载体类型: | Bacterial Expression, CRISPR |
载体抗性: | Kanamycin |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 低拷贝 |
插入基因信息 |
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插入基因名称: | VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf) |
物种来源: | Vibrio cholera |
克隆方法: | Gibson Cloning |
文献引用方法 |
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材料和方法部分: | pCgVchCAST-crtYf was a gift from Sheng Yang (Addgene plasmid # 203816 ; http://n2t.net/addgene:203816 ; RRID:Addgene_203816) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCgVchCAST-crtYf 14906 bp ds-DNA circular SYN 07-AUG-2023 DEFINITION Expression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coli. ACCESSION . VERSION . KEYWORDS pCgVchCAST-crtYf SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14906) TITLE Sheng Yang Plasmids 2023 REFERENCE 2 (bases 1 to 14906) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 7, 2023 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..14906 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 19..46 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 138..223 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(304..927) /codon_start=1 /gene="tetR from transposon Tn10" /product="tetracycline repressor TetR" /label=TetR /note="TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline." /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCKSGS" primer_bind 835..854 /label=Tet-R /note="Tetracycline resistance gene, reverse primer" protein_bind 1347..1365 /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O1 for the tetR and tetA genes" primer_bind complement(9916..9934) /label=T7 Term /note="T7 terminator, reverse primer" terminator 9930..9977 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(10206..11000) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(10317..10336) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 10927..10946 /label=Neo-R /note="Neomycin resistance gene, reverse primer" rep_origin complement(13959..14697) /direction=LEFT /label=CloDF13 ori /note="Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins." ORIGIN 1 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt aatttgatgc 61 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaacgttc 121 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata 181 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttaaattct agactgatga 241 atcccctaat gatttttatc aaaatcatta aggttaccat cacggaaaaa ggttatgctg 301 cttttaagac ccacttttac atttaagttg tttttctaat ccgcatatga tcaattcaag 361 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa 421 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc 481 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc 541 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata 601 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa 661 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa 721 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc 781 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag 841 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat 901 cactttactt ttatctaatc tagacatgtt gtgtctcctc taaagattgt aggaaatgca 961 atgtgtcaag cagttaggtc aatcagagca tcggtcactc aagaattctt gagctgacag 1021 aacccaaggg cgagtatggc gaatttttcc aactcacgca ccaaacctac tcacattgga 1081 taattcgtcg agtcaactcc gtgtcaacct gtcctcaact ttagcgggga aacaaagatt 1141 tcgcagcaga tgcaaaatca tacagacaca ttcaaaaatg gacattgacc ccaaacgggg 1201 gcatttccaa cacttgcgct aaatagtttt tgcagggaac gaacctgaaa tcgttcgcac 1261 tacgatctgc tcccccgaat ggggttaatt atgaactaca ttacaccccc gtgaatcgaa 1321 ttccaaaaac taaaaaaaat attgacactc tatcattgat agagtataat taacgggatc 1381 cactcttcat tgataagtcg ccaagcttgg gatccccagc ttgttgatac actaatgctt 1441 ttatataggg aaaaggtggt gaactactcc atgggtgaac tgccgagtag gtagctgata 1501 acaggcaacc atagggcagg aatcagaagt actggtgaac tgccgagtag gtagctgata 1561 acggatccga attcgagctc ggcgcgcctg caggtcgaca agcttgcggc cgctagggaa 1621 aaggtggtga actactatgt tcctgcagcg cccaaagcca tactccgatg agtccctcga 1681 atccttcttc atccgcgtgg ctaacaagaa cggctacggc gacgtccacc gcttcctgga 1741 ggcaaccaag cgcttcctcc aggatatcga ccacaacggc taccagacct tcccaaccga 1801 tatcacccgc atcaacccat actccgccaa gaactcctcc tccgctcgca ccgcatcctt 1861 cctgaagctc gcccagctga ccttcaacga gccaccagaa ctgctcggcc tcgctatcaa 1921 ccgcaccaac atgaagtact ccccatccac ctccgcagtg gtccgtggtg cagaagtgtt 1981 cccacgttcc ctgctccgca cccactccat cccatgctgc ccactgtgcc tccgcgagaa 2041 cggctacgct tcctacctgt ggcacttcca gggctacgaa tactgccact cccacaacgt 2101 gccactcatc accacctgct cctgcggcaa ggagttcgac taccgcgtct ccggcctgaa 2161 gggcatctgc tgcaagtgca aggaaccaat caccctcacc tcccgcgaga acggccacga 2221 agcagcctgc accgtgtcca actggctggc tggccacgag tccaagccac tgccaaacct 2281 cccaaagtcc taccgttggg gtctggtgca ctggtggatg ggcatcaagg attccgaatt 2341 cgaccacttc tccttcgtcc agttcttctc caactggcca cgctccttcc actccatcat 2401 cgaagatgag gtcgaattca acctggagca cgcagtggtc tccacctccg aactgcgtct 2461 caaggacctg ctcggtcgtc tgttcttcgg ctccatccgc ctgccagaac gcaacctcca 2521 gcacaacatc atcctcggcg agctgctctg ctacctggaa aaccgcctct ggcaggataa 2581 gggcctgatc gcaaacctca agatgaacgc cctggaggct accgtgatgc tgaactgctc 2641 cctcgaccag atcgcctcca tggtcgaaca gcgcatcctg aagccaaacc gcaagtccaa 2701 gccaaactcc ccactggatg tgaccgacta cctcttccac ttcggcgata tcttctgcct 2761 gtggctcgca gagttccagt ccgacgaatt caaccgctcc ttctacgtct cgaggtggta 2821 aatgcagacc ctgaaggagc tcatcgcctc caacccagat gacctgacca ccgaactcaa 2881 gcgcgctttc cgcccactga ccccacacat cgcaatcgat ggcaacgagc tggacgccct 2941 caccatcctg gtgaacctca ccgataagac cgatgaccag aaggatctgc tcgaccgcgc 3001 aaagtgcaag cagaagctgc gcgacgaaaa gtggtgggcc tcctgcatca actgcgtgaa 3061 ctaccgccag tcccacaacc caaagttccc agatatccgt tccgagggtg tcatccgtac 3121 ccaggcactg ggcgaactcc catccttcct gctctcctcc tccaagatcc caccatacca 3181 ctggtcctac tcccacgatt ccaagtacgt caacaagtcc gccttcctga ccaacgaatt 3241 ctgctgggac ggcgagatct cctgcctggg tgaactgctc aaggatgctg accacccact 3301 ctggaacacc ctgaagaagc tcggctgctc ccagaagacc tgcaaggcta tggcaaagca 3361 gctcgctgat atcaccctga ccaccatcaa cgtgaccctc gcaccaaact acctgaccca 3421 gatctccctc ccagattccg acacctccta tatctccctg tccccagtcg cttccctctc 3481 catgcagtcc cacttccacc agcgcctgca ggatgaaaac cgccactccg caatcacccg 3541 cttctcccgc accaccaaca tgggtgtgac cgctatgacc tgcggtggtg cattccgcat 3601 gctgaagtcc ggcgcaaagt tctcctcccc accacaccac cgcctgaact ccaagcgctc 3661 ctggctcacc tccgaacacg tgcagtccct gaagcagtac cagcgcctga acaagtccct 3721 catcccagag aactcccgca tcgcactgcg ccgcaagtac aagatcgaac tccagaacat 3781 ggtccgctcc tggttcgcca tgcaggatca caccctggac tccaacatcc tgatccagca 3841 cctcaaccac gatctgtcct acctcggcgc caccaagcgc ttcgcatacg acccagccat 3901 gaccaagctg ttcaccgaac tgctcaagcg cgagctctcc aactccatca acaacggcga 3961 gcagcacacc aacggctcct tcctggtgct cccaaacatc cgtgtctgcg gtgcaaccgc 4021 tctgtcctcc ccagtgaccg tcggcatccc atccctcacc gccttcttcg gcttcgtgca 4081 cgctttcgaa cgcaacatca accgcaccac ctcctccttc cgcgtcgagt ccttcgctat 4141 ctgcgtgcac cagctgcacg tcgaaaagcg cggcctcacc gcagaattcg tggagaaggg 4201 cgatggcacc atctccgcac cagccacccg cgatgactgg cagtgcgacg tggtcttctc 4261 cctgatcctc aacaccaact tcgcccagca catcgatcag gacaccctgg tcacctccct 4321 cccaaagcgt ctggctcgtg gttccgcaaa gatcgctatc gatgacttca agcacatcaa 4381 ctccttctcc accctggaaa ccgctatcga gtccctccca atcgaggcag gtcgttggct 4441 gtccctctac gcacagtcca acaacaacct gtccgatctg ctcgcagcca tgaccgaaga 4501 ccaccagctc atggcatcct gcgtgggcta ccacctgctc gaagagccaa aggataagcc 4561 aaactccctg cgcggctaca agcacgctat cgcagagtgc atcatcggcc tcatcaactc 4621 catcaccttc tcctccgaaa ccgacccaaa caccatcttc tggtccctga agaactacca 4681 gaactacctc gtggtccagc caaggagtat caacgatgaa actaccgaca aatctagcct 4741 atgagcgctc catcgatcca tccgacgtct gcttcttcgt ggtctggcca gatgaccgca 4801 agaccccact cacctacaac tcccgcaccc tgctcggcca gatggaagca gcctccctgg 4861 cttacgatgt gtccggccag ccaatcaagt ccgcaaccgc tgaggcactc gcacagggta 4921 acccacacca ggtggacttc tgccacgtcc catacggcgc atcccacatc gaatgctcct 4981 tctccgtctc cttctcctcc gagctgcgcc agccatacaa gtgcaactcc tccaaggtga 5041 agcagaccct ggtgcagctc gtcgaactgt acgagaccaa gatcggctgg accgaactcg 5101 ccacccgcta cctgatgaac atctgcaacg gcaagtggct ctggaagaac acccgcaagg 5161 cttactgctg gaacatcgtc ctgaccccat ggccatggaa cggcgaaaaa gtgggcttcg 5221 aggatatccg caccaactac acctcccgcc aggacttcaa gaacaacaag aactggtccg 5281 caatcgtcga aatgatcaag accgccttct cctccaccga tggcctggcc atcttcgagg 5341 tgcgcgctac cctgcacctc ccaaccaacg ccatggtgcg cccatcccag gtcttcaccg 5401 aaaaggagtc cggctccaag tccaagtcca agacccagaa ctcccgcgtc ttccagtcca 5461 ccaccatcga cggtgaacgt tccccaatcc tcggtgcttt caagaccggc gctgcaatcg 5521 caaccatcga tgactggtac ccagaagcaa ccgagccact gcgtgtgggt cgtttcggtg 5581 tccaccgcga agatgtgacc tgctaccgcc acccatccac cggcaaggac ttcttctcca 5641 tcctccagca ggcagaacac tacatcgagg tgctgtccgc taacaagacc ccagcacagg 5701 agaccatcaa cgatatgcac ttcctcatgg ccaacctgat caagggcggc atgttccagc 5761 acaaaggaga ctgactgtga agtggtacta caagaccatc accttcctcc cagaactgtg 5821 caacaacgag tccctcgcag caaagtgcct ccgtgtgctg cacggcttca actaccagta 5881 cgaaacccgc aacatcggcg tctccttccc actctggtgc gatgctaccg tgggcaagaa 5941 gatctccttc gtctccaaga acaagatcga actggacctg ctcctgaagc agcactactt 6001 cgtgcagatg gagcagctcc agtacttcca catctccaac accgtgctgg tcccagagga 6061 ttgcacctac gtctccttcc gccgctgcca gtccatcgac aagctcaccg ctgcaggtct 6121 ggctcgtaag atccgtcgtc tcgaaaagcg tgcactgtcc cgtggcgagc agttcgatcc 6181 atcctccttc gcacagaagg aacacaccgc catcgctcac taccactccc tgggcgagtc 6241 ctccaagcag accaaccgca acttccgcct caacatccgc atgctgtccg aacagccacg 6301 cgagggcaac tccatcttct cctcctacgg cctctccaac tccgaaaact ccttccagcc 6361 agtgccactg atctaatagg gaaaaggtgg tgaactacta tggcaacctc cctgccaacc 6421 ccatccgcaa tcaccacctc cgccctggaa tacgcattcc acaccccagc acgcaacctg 6481 accaagtccc gcggcaagaa catccaccgc tacgtgtccg tgaagatgtc caagcgcatc 6541 accgtggaat ccaccctgga atgcgatgca tgctaccact tcgacttcga accatccatc 6601 gtgcgcttct gcgcacagcc aatccgcttc ctgtactacc tgaacggcca gtcccactcc 6661 tacgtgccag acttcctggt gcagttcgac accaacgaat ttgtgctgta cgaagtgaag 6721 tccgcatacg caaagaacaa gccagacttc gatgtggaat gggaagcaaa ggtgaaggcc 6781 gcaaccgaac tgggcctgga actggaactg gtggaagaat ccgacatccg cgacaccgtg 6841 gtgctgaaca acctgaagcg catgcaccgc tacgcctcca aggacgaact gaacaacgtg 6901 cacaactccc tgctgaagat catcaagtac aacggcgcac agtccgcacg ctgcctgggc 6961 gaacagctgg gcctgaaggg ccgcaccgtg ctgccaatcc tgtgcgacct gctgtcccgc 7021 tgcctgctgg atacccgcct ggataagcca ctgtccctgg aatcccgctt cgagttggcc 7081 agttatggct aagaagggct tctcctcctt ccaccgcaag gctgtgtcct cccaggatac 7141 cctcgaatcc atcgagctgg tgtcctccgc aaactgcctc gaatccgtca cctaccagga 7201 catctccgct ttcccagaga ccatcgcagt cgaaatcaac ttccgcctgt ccatcctccg 7261 cttcctggca cgcaagtgcg aaaccatcgt ggccaagtcc atcgagccac accgcgtcga 7321 actgcagcag aactactccc gcaagatccc atccgccatc accatctacc gctggtggct 7381 cgctttccgc aagtccgatt acaacccaat ctccctggcc ccaaacatca aggaccgcgg 7441 caaccgcgaa accaaggtgt ccaccgtggt cgattccatc atggagcagg cagtggaacg 7501 cgtcatctcc ggccgcaagg tgaacgtctc ctccgcatac aagcgtgtgc gtcgtaaggt 7561 ccgccagtac aacctgaccc acggcaccaa gtacacctac ccaaagtacg agtccgtgcg 7621 caagcgcgtg aagaagaaga ccccattcga actgctcgca gcaggcaagg gcgagcgtgt 7681 ggctaagcgc gaattccgcc gcatgggcaa gaagatcctc acctcctccg tgctggaacg 7741 cgtcgagatc gatcacaccg tggtcgacct cttcgcagtc cacgaagagt accgtatccc 7801 actgggtcgt ccatggctca cccagctggt ggattgctac tccaaggccg tcatcggctt 7861 ctacctgggc ttcgagccac catcctacgt gtccgtctcc ctggcactca agaacgccat 7921 ccagcgcaag gatgacctca tctcctccta cgaatccatc gaaaacgagt ggctgtgcta 7981 cggcatccca gatctgctcg tgaccgacaa cggcaaggag ttcctgtcca aggctttcga 8041 tcaggcatgc gaatccctgc tcatcaacgt gcaccagaac aaggtcgaga ccccagacaa 8101 caagccacac gtggaacgca actacggcac catcaacacc tccctgctcg atgacctccc 8161 tggcaagtcc ttctcccagt acctgcagcg cgagggctac gattccgtcg gcgaagcaac 8221 cctgaccctc aacgaaatcc gcgagatcta cctcatctgg ctggtggaca tctaccacaa 8281 gaagccaaac cagcgcggca ccaactgccc aaacgtcgct tggaagaagg gctgccagga 8341 atgggagcca gaagagttct ccggctccaa ggatgaactc gacttcaagt tcgcaatcgt 8401 ggattacaag cagctgacca aagtgggcat caccgtctac aaggagctct cctactccaa 8461 cgaccgcctg gccgaatacc gcggcaagaa gggcaaccac aaggtgcagt tcaagtacaa 8521 cccagagtgc atggctgtga tctgggtcct ggatgaggac atgaacgaat acttcaccgt 8581 gaacgccatc gattacgaat acgcttcccg cgtctccctc tggcagcaca agtacaacat 8641 gaagtaccag gccgagctga actccgctga atacgatgag gacaaggaaa tcgatgctga 8701 aatcaagatc gaagagatcg cagaccgctc catcgtgaag accaacaaga tccgtgcacg 8761 tcgtcgtggt gcacgtcacc aggaaaactc cgctcgcgca aagtccatct ccaacgccaa 8821 cccagcttcc atccagaagc acgaagatga gatcgtgtcc gccgacaacg atgactggga 8881 tatcgactac gtctgagaaa tcgtcaaatg tccgaaaccc gtgaggctcg tatctcccgt 8941 gctaagcgcg cattcgtgtc caccccatcc gtccgcaaga tcctctccta catggatcgc 9001 tgccgcgatc tgtccgacct cgaatccgag ccaacctgca tgatggtgta cggtgcatcc 9061 ggtgtgggca agaccaccgt catcaagaag tacctgaacc agaaccgtcg tgaatccgag 9121 gcaggtggtg acatcatccc agtcctgcac atcgaactcc cagataacgc taagccagtg 9181 gacgcagccc gcgaactgct cgtcgagatg ggcgatccac tggctctcta cgaaaccgac 9241 ctggcacgcc tcaccaagcg tctgaccgag ctcatcccag cagtgggcgt caagctgatc 9301 atcatcgatg aattccagca cctcgtggaa gagcgttcca accgtgtgct gacccaggtc 9361 ggcaactggc tgaagatgat cctcaacaag accaagtgcc caatcgtcat cttcggcatg 9421 ccatactcca aggtggtcct gcaggctaac tcccagctcc acggccgctt ctccatccag 9481 gtggaactgc gtccattctc ctaccagggt ggtcgtggcg tcttcaagac cttcctggag 9541 tacctcgaca aggcactgcc attcgaaaag caggccggcc tcgctaacga gtccctgcag 9601 aagaagctct acgccttctc ccagggcaac atgcgctccc tgcgcaacct catctaccag 9661 gcatccatcg aagccatcga taaccagcac gagaccatca ccgaagagga cttcgtgttc 9721 gcatccaagc tgacctccgg cgataagcca aactcctgga agaacccatt cgaagagggc 9781 gtggaagtca ccgaggacat gctccgccca ccaccaaagg atatcggctg ggaagactac 9841 ctgcgtcact ccaccccacg tgtgtccaag ccaggtcgca acaagaactt cttcgagtaa 9901 cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg 9961 tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc 10021 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc 10081 ctgaaccgac gacaagctga cgaccggtgg cacttttcgg ggaaatgtgc gcggaacccc 10141 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcaagct 10201 tcatatcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg 10261 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata 10321 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg 10381 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg 10441 gtcacgacga gatcctcgcc gtcgggcatc cgcgccttga gcctggcgaa cagttcggct 10501 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc 10561 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga 10621 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca 10681 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc 10741 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat 10801 agccgcgctg cctcgtcttg gagttcattc agggcaccgg acaggtcggt cttgacaaaa 10861 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc 10921 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc 10981 aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc agatcttgat 11041 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc 11101 ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc 11161 gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt 11221 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc 11281 tgcggactgg ctttctacgt gttccgcttc cggtgaccgc ctcgatgatc gccgggtggg 11341 cgtggcccaa ggaggatcat ccagccattc ggggtcgttc actggttccc ctttctgatt 11401 tctggcatag aagaaccccc gtgaactgtg tggttccggg ggttgctgat ttttgcgaga 11461 cttctcgcgc aattccctag cttaggtgaa aacaccatga aacactaggg aaacacccat 11521 gaaacaccca ttagggcagt agggcggctt cttcgtctag ggcttgcatt tgggcggtga 11581 tctggtcttt agcgtgtgaa agtgtgtcgt aggtggcgtg ctcaatgcac tcgaacgtca 11641 cgtcatttac cgggtcacgg tgggcaaaga gaactagtgg gttagacatt gttttcctcg 11701 ttgtcggtgg tggtgagctt ttctagccgc tcggtaaacg cggcgatcat gaactcttgg 11761 aggttttcac cgttctgcat gcctgcgcgc ttcatgtcct cacgtagtgc caaaggaacg 11821 cgtgcggtga ccacgacggg cttagccttt gcctgcgctt ctagtgcttc gatggtggct 11881 tgtgcctgcg cttgctgcgc ctgtagtgcc tgttgagctt cttgtagttg ctgttctagc 11941 tgtgccttgg ttgccatgct ttaagactct agtagctttc ctgcgatatg tcatgcgcat 12001 gcgtagcaaa cattgtcctg caactcattc attatgtgca gtgctcctgt tactagtcgt 12061 acatactcat atttacctag tctgcatgca gtgcatgcac atgcagtcat gtcgtgctaa 12121 tgtgtaaaac atgtacatgc agattgctgg gggtgcaggg ggcggagcca ccctgtccat 12181 gcggggtgtg gggcttgccc cgccggtaca gacagtgagc accggggcac ctagtcgcgg 12241 ataccccccc taggtatcgg acacgtaacc ctcccatgtc gatgcaaatc tttaacattg 12301 agtacgggta agctggcacg catagccaag ctaggcggcc accaaacacc actaaaaatt 12361 aatagtccct agacaagaca aacccccgtg cgagctacca actcatatgc acgggggcca 12421 cataacccga aggggtttca attgacaacc atagcactag ctaagacaac gggcacaaca 12481 cccgcacaaa ctcgcactgc gcaaccccgc acaacatcgg gtctaggtaa cactgaaata 12541 gaagtgaaca cctctaagga accgcaggtc aatgagggtt ctaaggtcac tcgcgctagg 12601 gcgtggcgta ggcaaaacgt catgtacaag atcaccaata gtaaggctct ggcggggtgc 12661 cataggtggc gcagggacga agctgttgcg gtgtcctggt cgtctaacgg tgcttcgcag 12721 tttgagggtc tgcaaaactc tcactctcgc tgggggtcat ctctggctga attggaagtc 12781 atgggcgaac gccgcattga gctggctatt gctactaaga atcacttggc ggcgggtggc 12841 gcgctcatga tgtttgtggg cactgttcga cacaaccgct cacagtcatt tgcgcaggtt 12901 gaagcgggta ttaagactgc gtactcttcg atggtgaaaa catctcagtg gaagaaagaa 12961 cgtgcacggt acggggtgga gcacacctat agtgactatg aggtcacaga ctcttgggcg 13021 aacggttggc acttgcaccg caacatgctg ttgttcttgg atcgtccact gtctgacgat 13081 gaactcaagg cgtttgagga ttccatgttt tcccgctggt ctgctggtgt ggttaaggcc 13141 ggtatggacg cgccactgcg tgagcacggg gtcaaacttg atcaggtgtc tacctggggt 13201 ggagacgctg cgaaaatggc aacctacctc gctaagggca tgtctcagga actgactggc 13261 tccgctacta aaaccgcgtc taaggggtcg tacacgccgt ttcagatgtt ggatatgttg 13321 gccgatcaaa gcgacgccgg cgaggatatg gacgctgttt tggtggctcg gtggcgtgag 13381 tatgaggttg gttctaaaaa cctgcgttcg tcctggtcac gtggggctaa gcgtgctttg 13441 ggcattgatt acatagacgc tgatgtacgt cgtgaaatgg aagaagaact gtacaagctc 13501 gccggtctgg aagcaccgga acgggtcgaa tcaacccgcg ttgctgttgc tttggtgaag 13561 cccgatgatt ggaaactgat tcagtctgat ttcgcggtta ggcagtacgt tctcgattgc 13621 gtggataagg ctaaggacgt ggccgctgcg caacgtgtcg ctaatgaggt gctggcaagt 13681 ctgggtgtgg attccacccc gtgcatgatc gttatggatg atgtggactt ggacgcggtt 13741 ctgcctactc atggggacgc tactaagcgt gatctgaatg cggcggtgtt cgcgggtaat 13801 gagcagacta ttcttcgcac ccactaaaag cggcataaac cccgttcgat attttgtgcg 13861 atgaatttat ggtcaatgtc gcgggggcaa actatgatgg gtcttgttgt ttagctagct 13921 cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt 13981 tacccacaga ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc 14041 gccggtggcg tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt 14101 ttccggtgca tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac 14161 ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg 14221 ttccgaccct gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc 14281 gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg 14341 ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca 14401 cttgagtcca acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg 14461 gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt 14521 ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt 14581 taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg 14641 tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 14701 tctactgaac cgctctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc 14761 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg 14821 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga 14881 gctaacttac attaattgcg ttgcgc //
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