| 质粒名称: | pON.mCherry |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 84821 |
| 存储实验室: | Howard Shuman |
|
载体骨架基本信息 |
|
| 载体名称: | pMMB207c |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 9077 bp |
| 完整质粒大小: | 9785 bp |
| 载体修饰: | lac阻遏物结合位点（操作者）发生突变，可能使阻遏物与DNA的结合失效，从而导致质粒携带的lac启动子的结构性表达。 |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mCherry |
| 物种来源: | -- |
| 插入大小: | 708 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Tac Promoter |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI or NdeI(未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | TCGGCTCGTATAATGTGTGGAATTGTG |
| 3' 测序引物 | CAGACCGCTTCTGCGTTCTGAT |
|
文献引用方法 |
|
| 材料和方法部分: | pON.mCherry was a gift from Howard Shuman (Addgene plasmid # 84821 ; http://n2t.net/addgene:84821 ; RRID:Addgene_84821) |
| 参考文献部分: | Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria. Gebhardt MJ, Jacobson RK, Shuman HA. PLoS One. 2017 Mar 3;12(3):e0173116. doi: 10.1371/journal.pone.0173116. eCollection 2017. PONE-D-16-41433 [pii] PubMed 28257493 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pON.mCherry 9785 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Constitutive Expression plasmid with mCherry fluorescent protein.
ACCESSION .
VERSION .
KEYWORDS pON.mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9785)
AUTHORS Gebhardt MJ, Jacobson RK, Shuman HA
TITLE Seeing red; the development of pON.mCherry, a broad-host range
constitutive expression plasmid for Gram-negative bacteria.
JOURNAL PLoS One. 2017 Mar 3;12(3):e0173116. doi:
10.1371/journal.pone.0173116. eCollection 2017.
PUBMED 28257493
REFERENCE 2 (bases 1 to 9785)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9785
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 3..31
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
primer_bind complement(871..888)
/label=pBAD Reverse
/note="For vectors with E. coli araBAD promoter, reverse
primer"
terminator 1041..1127
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1219..1246
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1265..1356
/gene="bla"
/label=AmpR promoter
promoter 1807..1909
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 1910..2569
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(1976..1995)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
rep_origin 2901..3295
/label=RSF1010 oriV
/note="replication origin of the broad-host-range plasmid
RSF1010 from Salmonella enterica serovar Typhimurium;
requires the RSF1010 RepA/B/C proteins for replication"
CDS 4963..5934
/codon_start=1
/gene="repB"
/product="replication protein B of the broad-host-range
plasmid RSF1010 from Salmonella enterica serovar
Typhimurium"
/label=RSF1010 RepB
/translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
CDS 6445..7284
/codon_start=1
/gene="repA"
/product="replication protein A of the broad-host-range
plasmid RSF1010 from Salmonella enterica serovar
Typhimurium"
/label=RSF1010 RepA
/translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
CDS 7271..8122
/codon_start=1
/gene="repC"
/product="replication protein C of the broad-host-range
plasmid RSF1010 from Salmonella enterica serovar
Typhimurium"
/label=RSF1010 RepC
/translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
AG"
primer_bind 9442..9461
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(9481..9558)
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
1 tgttgacaat taatcatcgg ctcgtataat gtgtggaaag gcggttcacc gccgttttca
61 cacaggaaac agaattcttt aagaaggaga tatacatatg gtttccaagg gcgaggagga
121 taacatggct atcattaaag agttcatgcg cttcaaagtt cacatggagg gttctgttaa
181 cggtcacgag ttcgagatcg aaggcgaagg cgagggccgt ccgtatgaag gcacccagac
241 cgccaaactg aaagtgacta aaggcggccc gctgcctttt gcgtgggaca tcctgagccc
301 gcaatttatg tacggttcta aagcgtatgt taaacaccca gcggatatcc cggactatct
361 gaagctgtct tttccggaag gtttcaagtg ggaacgcgta atgaattttg aagatggtgg
421 tgtcgtgacc gtcactcagg actcctccct gcaggatggc gagttcatct ataaagttaa
481 actgcgtggt actaattttc catctgatgg cccggtgatg cagaaaaaga cgatgggttg
541 ggaggcgtct agcgaacgca tgtatccgga agatggtgcg ctgaaaggcg aaattaaaca
601 gcgcctgaaa ctgaaagatg gcggccatta tgacgctgaa gtgaaaacca cgtacaaagc
661 caagaaacct gtgcagctgc ctggcgcgta caatgtgaat attaaactgg acatcacctc
721 tcataatgaa gattatacga tcgtagagca atatgagcgc gcggagggtc gtcattctac
781 cggtggcatg gatgagctgt acaaataatc tagagtcgac ctgcaggcat gcaagcttgg
841 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
901 cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat
961 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
1021 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
1081 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
1141 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
1201 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
1261 ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
1321 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
1381 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
1441 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
1501 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgtgatgt ccggcggtgc
1561 ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga gggacagctg atagaaacag
1621 aagccactgg agcaagcacc tcaaaaacac catcatacac taaatcagta agttggcagc
1681 atcacccgac gcactttgcg ccgaataaat acctgtgacg gaagatcact tcgcagaata
1741 aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg
1801 agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
1861 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
1921 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
1981 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
2041 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
2101 ccgcctgatg aatgctcatc cggaattacg tatggcaatg aaagacggtg agctggtgat
2161 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
2221 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
2281 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
2341 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
2401 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
2461 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
2521 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg
2581 cagttattgt cttcaaattc ccgttgcaca tagcccggca attcctttcc ctgctctgcc
2641 ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag ggtttgctcg
2701 ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct ggccgccaca
2761 tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg cttagcggaa
2821 agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt
2881 cactcccgta ctaactgtca cgaacccctg caataactgt cacgcccccc tgcaataact
2941 gtcacgaacc cctgcaataa ctgtcacgcc cccaaacctg caaacccagc aggggcgggg
3001 gctggcgggg tgttggaaaa atccatccat gattatctaa gaataatcca ctaggcgcgg
3061 ttatcagcgc ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc agaggccgga
3121 tagctggtct attcgctgcg ctaggctaca caccgcccca ccgctgcgcg gcagggggaa
3181 aggcgggcaa agcccgctaa accccacacc aaaccccgca gaaatacgct ggagcgcttt
3241 tagccgcttt agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca
3301 gggcctgtct cggtcgatca ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa
3361 caaggcgcgg tcgtggtcgc gttcaaggta cgcatccatt gccgccatga gccgatcctc
3421 cggccactcg ctgctgttca ccttggccaa aatcatggcc cccaccagca ccttgcgcct
3481 tgtttcgttc ttgcgctctt gctgctgttc ccttgcccgc tcccgctgaa tttcggcatt
3541 gattcgcgct cgttgttctt cgagcttggc cagccgatcc gccgccttgt tgctcccctt
3601 aaccatcttg acaccccatt gttaatgtgc tgtctcgtag gctatcatgg aggcacagcg
3661 gcggcaatcc cgaccctact ttgtagggga gggcgcactt accggtttct cttcgagaaa
3721 ctggcctaac ggccaccctt cgggcggtgc gctctccgag ggccattgca tggagccgaa
3781 aagcaaaagc aacagcgagg cagcatggcg atttatcacc ttacggcgaa aaccggcagc
3841 aggtcgggcg gccaatcggc cagggccaag gccgactaca tccagcgcga aggcaagtat
3901 gcccgcgaca tggatgaagt cttgcacgcc gaatccgggc acatgccgga gttcgtcgag
3961 cggcccgccg actactggga tgctgccgac ctgtatgaac gcgccaatgg gcggctgttc
4021 aaggaggtcg aatttgccct gccggtcgag ctgaccctcg accagcagaa ggcgctggcg
4081 tccgagttcg cccagcacct gaccggtgcc gagcgcctgc cgtatacgct ggccatccat
4141 gccggtggcg gcgagaaccc gcactgccac ctgatgatct ccgagcggat caatgacggc
4201 atcgagcggc ccgccgctca gtggttcaag cggtacaacg gcaagacccc ggagaagggc
4261 ggggcacaga agaccgaagc gctcaagccc aaggcatggc ttgagcagac ccgcgaggca
4321 tgggccgacc atgccaaccg ggcattagag cgggctggcc acgacgcccg cattgaccac
4381 agaacacttg aggcgcaggg catcgagcgc ctgcccggtg ttcacctggg gccgaacgtg
4441 gtggagatgg aaggccgggg catccgcacc gaccgggcag acgtggccct gaacatcgac
4501 accgccaacg cccagatcat cgacttacag gaataccggg aggcaataga ccatgaacgc
4561 aatcgacaga gtgaagaaat ccagaggcat caacgagtta gcggagcaga tcgaaccgct
4621 ggcccagagc atggcgacac tggccgacga agcccggcag gtcatgagcc agaccaagca
4681 ggccagcgag gcgcaggcgg cggagtggct gaaagcccag cgccagacag gggcggcatg
4741 ggtggagctg gccaaagagt tgcgggaggt agccgccgag gtgagcagcg ccgcgcagag
4801 cgcccggagc gcgtcgcggg ggtggcactg gaagctatgg ctaaccgtga tgctggcttc
4861 catgatgcct acggtggtgc tgctgatcgc atcgttgctc ttgctcgacc tgacgccact
4921 gacaaccgag gacggctcga tctggctgcg cttggtggcc cgatgaagaa cgacaggact
4981 ttgcaggcca taggccgaca gctcaaggcc atgggctgtg agcgcttcga tatcggcgtc
5041 agggacgcac ccaccggcca gatgatgaac cgggaatggt cagccgccga agtgctccag
5101 aacacgccat ggctcaagcg gatgaatgcc cagggcaatg acgtgtatat caggcccgcc
5161 gagcaggagc ggcatggtct ggtgctggtg gacgacctca gcgagtttga cctggatgac
5221 atgaaagccg agggccggga gcctgccctg gtagtggaaa ccagcccgaa gaactatcag
5281 gcatgggtca aggtggccga cgccgcaggc ggtgaacttc gggggcagat tgcccggacg
5341 ctggccagcg agtacgacgc cgacccggcc agcgccgaca gccgccacta tggccgcttg
5401 gcgggcttca ccaaccgcaa ggacaagcac accacccgcg ccggttatca gccgtgggtg
5461 ctgctgcgtg aatccaaggg caagaccgcc accgctggcc cggcgctggt gcagcaggct
5521 ggccagcaga tcgagcaggc ccagcggcag caggagaagg cccgcaggct ggccagcctc
5581 gaactgcccg agcggcagct tagccgccac cggcgcacgg cgctggacga gtaccgcagc
5641 gagatggccg ggctggtcaa gcgcttcggt catgacctca gcaagtgcga ctttatcgcc
5701 gcgcagaagc tggccagccg gggccgcagt gccgaggaaa tcggcaaggc catggccgag
5761 gccagcccag cgctggcaga gcgcaagccc ggccacgaag cggattacat cgagcgcacc
5821 gtcagcaagg tcatgggtct gcccagcgtc cagcttgcgc gggccgagct ggcacgggca
5881 ccggcacccc gccagcgagg catggacagg ggcgggccag atttcagcat gtagtgcttg
5941 cgttggtact cacgcctgtt atactatgag tactcacgca cagaaggggg ttttatggaa
6001 tacgaaaaaa gcgcttcagg gtcggtctac ctgatcaaaa gtgacaaggg ctattggttg
6061 cccggtggct ttggttatac gtcaaacaag gccgaggctg gccgcttttc agtcgctgat
6121 atggccagcc ttaaccttga cggctgcacc ttgtccttgt tccgcgaaga caagcctttc
6181 ggccccggca agtttctcgg tgactgatat gaaagaccaa aaggacaagc agaccggcga
6241 cctgctggcc agccctgacg ctgtacgcca agcgcgatat gccgagcgca tgaaggccaa
6301 agggatgcgt cagcgcaagt tctggctgac cgacgacgaa tacgaggcgc tgcgcgagtg
6361 cctggaagaa ctcagagcgg cgcagggcgg gggtagtgac cccgccagcg cctaaccacc
6421 aactgcctgc aaaggaggca atcaatggct acccataagc ctatcaatat tctggaggcg
6481 ttcgcagcag cgccgccacc gctggactac gttttgccca acatggtggc cggtacggtc
6541 ggggcgctgg tgtcgcccgg tggtgccggt aaatccatgc tggccctgca actggccgca
6601 cagattgcag gcgggccgga tctgctggag gtgggcgaac tgcccaccgg cccggtgatc
6661 tacctgcccg ccgaagaccc gcccaccgcc attcatcacc gcctgcacgc ccttggggcg
6721 cacctcagcg ccgaggaacg gcaagccgtg gctgacggcc tgctgatcca gccgctgatc
6781 ggcagcctgc ccaacatcat ggccccggag tggttcgacg gcctcaagcg cgccgccgag
6841 ggccgccgcc tgatggtgct ggacacgctg cgccggttcc acatcgagga agaaaacgcc
6901 agcggcccca tggcccaggt catcggtcgc atggaggcca tcgccgccga taccgggtgc
6961 tctatcgtgt tcctgcacca tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag
7021 cagcaggcca gccggggcag ctcggtactg gtcgataaca tccgctggca gtcctacctg
7081 tcgagcatga ccagcgccga ggccgaggaa tggggtgtgg acgacgacca gcgccggttc
7141 ttcgtccgct tcggtgtgag caaggccaac tatggcgcac cgttcgctga tcggtggttc
7201 aggcggcatg acggcggggt gctcaagccc gccgtgctgg agaggcagcg caagagcaag
7261 ggggtgcccc gtggtgaagc ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc
7321 ggcgcactgt ctggcccccg gcctgttccg tgccctcaag cggggcgagc gcaagcgcag
7381 caagctggac gtgacgtatg actacggcga cggcaagcgg atcgagttca gcggcccgga
7441 gccgctgggc gctgatgatc tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa
7501 tggcctagtg cttggcccgg aacccaagac cgaaggcgga cggcagctcc ggctgttcct
7561 ggaacccaag tgggaggccg tcaccgctga atgccatgtg gtcaaaggta gctatcgggc
7621 gctggcaaag gaaatcgggg cagaggtcga tagtggtggg gcgctcaagc acatacagga
7681 ctgcatcgag cgcctttgga aggtatccat catcgcccag aatggccgca agcggcaggg
7741 gtttcggctg ctgtcggagt acgccagcga cgaggcggac gggcgcctgt acgtggccct
7801 gaaccccttg atcgcgcagg ccgtcatggg tggcggccag catgtgcgca tcagcatgga
7861 cgaggtgcgg gcgctggaca gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg
7921 gatcgacccc ggcaaaaccg gcaaggcttc catagatacc ttgtgcggct atgtctggcc
7981 gtcagaggcc agtggttcga ccatgcgcaa gcgccgcaag cgggtgcgcg aggcgttgcc
8041 ggagctggtc gcgctgggct ggacggtaac cgagttcgcg gcgggcaagt acgacatcac
8101 ccggcccaag gcggcaggct gacccccccc actctattgt aaacaagaca tttttatctt
8161 ttatattcaa tggcttattt tcctgctaat tggtaatacc atgaaaaata ccatgctcag
8221 aaaaggctta acaatatttt gaaaaattgc ctactgagcg ctgccgcaca gctccatagg
8281 ccgctttcct ggctttgctt ccagatgtat gctcttctgc tcccgaacgc cagcaagacg
8341 tagcccagcg cgtcggccag cttgcaattc gcgctaactt acattaattg cgttgcgctc
8401 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
8461 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
8521 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
8581 cgtggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca
8641 tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc
8701 ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc
8761 agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact
8821 ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca
8881 gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg
8941 ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa
9001 aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt
9061 gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc
9121 actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg
9181 ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc
9241 gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga
9301 ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc
9361 cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga
9421 aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac
9481 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
9541 gcaccattcg atggtgtcaa cgtaaatgcc gcttcgcctt cgcgcgcgaa ttgcaagctg
9601 atccgggctt atcgactgca cggtgcacca atgcttctgg cgtcaggcag ccatcggaag
9661 ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact
9721 cccgttctgg ataatgtttt ttgcgccgac atcataacgg ttctggcaaa tattctgaaa
9781 tgagc
//
