pON.mCherry

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pON.mCherry 
出品公司: 爱迪基因
目录编号: 84821
存储实验室: Howard Shuman

载体骨架基本信息
载体名称: pMMB207c
载体出品公司: --
载体抗性: 氯霉素
空载体大小: 9077 bp
完整质粒大小: 9785 bp
载体修饰: lac阻遏物结合位点(操作者)发生突变,可能使阻遏物与DNA的结合失效,从而导致质粒携带的lac启动子的结构性表达。
载体类型: 细菌表达
筛选标记: --
高拷贝/低拷贝: 低拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: mCherry
物种来源: --
插入大小: 708 bp
基因突变: --
GenBank编号 --
启动子 Tac Promoter
标签/融合蛋白: --
克隆方法: 限制性内切酶
5' 克隆位点: EcoRI or NdeI(未破坏)
3' 克隆位点: XbaI(未破坏)
5' 测序引物 TCGGCTCGTATAATGTGTGGAATTGTG
3' 测序引物 CAGACCGCTTCTGCGTTCTGAT

文献引用方法
材料和方法部分: pON.mCherry was a gift from Howard Shuman (Addgene plasmid # 84821 ; http://n2t.net/addgene:84821 ; RRID:Addgene_84821)
参考文献部分: Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria. Gebhardt MJ, Jacobson RK, Shuman HA. PLoS One. 2017 Mar 3;12(3):e0173116. doi: 10.1371/journal.pone.0173116. eCollection 2017. PONE-D-16-41433 [pii] PubMed 28257493

载体图谱质粒图谱和多克隆位点信息

pON.mCherry质粒图谱

载体简介

载体序列

LOCUS       pON.mCherry                9785 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Constitutive Expression plasmid with mCherry fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    pON.mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Gebhardt MJ, Jacobson RK, Shuman HA
  TITLE     Seeing red; the development of pON.mCherry, a broad-host range 
            constitutive expression plasmid for Gram-negative bacteria.
  JOURNAL   PLoS One. 2017 Mar 3;12(3):e0173116. doi: 
            10.1371/journal.pone.0173116. eCollection 2017.
  PUBMED    28257493
REFERENCE   2  (bases 1 to 9785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        3..31
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     complement(871..888)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1041..1127
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1219..1246
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1265..1356
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1807..1909
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1910..2569
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1976..1995)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2901..3295
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010 from Salmonella enterica serovar Typhimurium; 
                     requires the RSF1010 RepA/B/C proteins for replication"
     CDS             4963..5934
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 from Salmonella enterica serovar 
                     Typhimurium"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6445..7284
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 from Salmonella enterica serovar 
                     Typhimurium"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7271..8122
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 from Salmonella enterica serovar 
                     Typhimurium"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     9442..9461
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9481..9558)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 tgttgacaat taatcatcgg ctcgtataat gtgtggaaag gcggttcacc gccgttttca
       61 cacaggaaac agaattcttt aagaaggaga tatacatatg gtttccaagg gcgaggagga
      121 taacatggct atcattaaag agttcatgcg cttcaaagtt cacatggagg gttctgttaa
      181 cggtcacgag ttcgagatcg aaggcgaagg cgagggccgt ccgtatgaag gcacccagac
      241 cgccaaactg aaagtgacta aaggcggccc gctgcctttt gcgtgggaca tcctgagccc
      301 gcaatttatg tacggttcta aagcgtatgt taaacaccca gcggatatcc cggactatct
      361 gaagctgtct tttccggaag gtttcaagtg ggaacgcgta atgaattttg aagatggtgg
      421 tgtcgtgacc gtcactcagg actcctccct gcaggatggc gagttcatct ataaagttaa
      481 actgcgtggt actaattttc catctgatgg cccggtgatg cagaaaaaga cgatgggttg
      541 ggaggcgtct agcgaacgca tgtatccgga agatggtgcg ctgaaaggcg aaattaaaca
      601 gcgcctgaaa ctgaaagatg gcggccatta tgacgctgaa gtgaaaacca cgtacaaagc
      661 caagaaacct gtgcagctgc ctggcgcgta caatgtgaat attaaactgg acatcacctc
      721 tcataatgaa gattatacga tcgtagagca atatgagcgc gcggagggtc gtcattctac
      781 cggtggcatg gatgagctgt acaaataatc tagagtcgac ctgcaggcat gcaagcttgg
      841 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
      901 cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat
      961 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
     1021 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1081 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
     1141 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
     1201 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
     1261 ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1321 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1381 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1441 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1501 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgtgatgt ccggcggtgc
     1561 ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga gggacagctg atagaaacag
     1621 aagccactgg agcaagcacc tcaaaaacac catcatacac taaatcagta agttggcagc
     1681 atcacccgac gcactttgcg ccgaataaat acctgtgacg gaagatcact tcgcagaata
     1741 aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg
     1801 agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
     1861 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     1921 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     1981 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     2041 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     2101 ccgcctgatg aatgctcatc cggaattacg tatggcaatg aaagacggtg agctggtgat
     2161 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     2221 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     2281 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     2341 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     2401 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     2461 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     2521 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg
     2581 cagttattgt cttcaaattc ccgttgcaca tagcccggca attcctttcc ctgctctgcc
     2641 ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag ggtttgctcg
     2701 ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct ggccgccaca
     2761 tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg cttagcggaa
     2821 agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt
     2881 cactcccgta ctaactgtca cgaacccctg caataactgt cacgcccccc tgcaataact
     2941 gtcacgaacc cctgcaataa ctgtcacgcc cccaaacctg caaacccagc aggggcgggg
     3001 gctggcgggg tgttggaaaa atccatccat gattatctaa gaataatcca ctaggcgcgg
     3061 ttatcagcgc ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc agaggccgga
     3121 tagctggtct attcgctgcg ctaggctaca caccgcccca ccgctgcgcg gcagggggaa
     3181 aggcgggcaa agcccgctaa accccacacc aaaccccgca gaaatacgct ggagcgcttt
     3241 tagccgcttt agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca
     3301 gggcctgtct cggtcgatca ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa
     3361 caaggcgcgg tcgtggtcgc gttcaaggta cgcatccatt gccgccatga gccgatcctc
     3421 cggccactcg ctgctgttca ccttggccaa aatcatggcc cccaccagca ccttgcgcct
     3481 tgtttcgttc ttgcgctctt gctgctgttc ccttgcccgc tcccgctgaa tttcggcatt
     3541 gattcgcgct cgttgttctt cgagcttggc cagccgatcc gccgccttgt tgctcccctt
     3601 aaccatcttg acaccccatt gttaatgtgc tgtctcgtag gctatcatgg aggcacagcg
     3661 gcggcaatcc cgaccctact ttgtagggga gggcgcactt accggtttct cttcgagaaa
     3721 ctggcctaac ggccaccctt cgggcggtgc gctctccgag ggccattgca tggagccgaa
     3781 aagcaaaagc aacagcgagg cagcatggcg atttatcacc ttacggcgaa aaccggcagc
     3841 aggtcgggcg gccaatcggc cagggccaag gccgactaca tccagcgcga aggcaagtat
     3901 gcccgcgaca tggatgaagt cttgcacgcc gaatccgggc acatgccgga gttcgtcgag
     3961 cggcccgccg actactggga tgctgccgac ctgtatgaac gcgccaatgg gcggctgttc
     4021 aaggaggtcg aatttgccct gccggtcgag ctgaccctcg accagcagaa ggcgctggcg
     4081 tccgagttcg cccagcacct gaccggtgcc gagcgcctgc cgtatacgct ggccatccat
     4141 gccggtggcg gcgagaaccc gcactgccac ctgatgatct ccgagcggat caatgacggc
     4201 atcgagcggc ccgccgctca gtggttcaag cggtacaacg gcaagacccc ggagaagggc
     4261 ggggcacaga agaccgaagc gctcaagccc aaggcatggc ttgagcagac ccgcgaggca
     4321 tgggccgacc atgccaaccg ggcattagag cgggctggcc acgacgcccg cattgaccac
     4381 agaacacttg aggcgcaggg catcgagcgc ctgcccggtg ttcacctggg gccgaacgtg
     4441 gtggagatgg aaggccgggg catccgcacc gaccgggcag acgtggccct gaacatcgac
     4501 accgccaacg cccagatcat cgacttacag gaataccggg aggcaataga ccatgaacgc
     4561 aatcgacaga gtgaagaaat ccagaggcat caacgagtta gcggagcaga tcgaaccgct
     4621 ggcccagagc atggcgacac tggccgacga agcccggcag gtcatgagcc agaccaagca
     4681 ggccagcgag gcgcaggcgg cggagtggct gaaagcccag cgccagacag gggcggcatg
     4741 ggtggagctg gccaaagagt tgcgggaggt agccgccgag gtgagcagcg ccgcgcagag
     4801 cgcccggagc gcgtcgcggg ggtggcactg gaagctatgg ctaaccgtga tgctggcttc
     4861 catgatgcct acggtggtgc tgctgatcgc atcgttgctc ttgctcgacc tgacgccact
     4921 gacaaccgag gacggctcga tctggctgcg cttggtggcc cgatgaagaa cgacaggact
     4981 ttgcaggcca taggccgaca gctcaaggcc atgggctgtg agcgcttcga tatcggcgtc
     5041 agggacgcac ccaccggcca gatgatgaac cgggaatggt cagccgccga agtgctccag
     5101 aacacgccat ggctcaagcg gatgaatgcc cagggcaatg acgtgtatat caggcccgcc
     5161 gagcaggagc ggcatggtct ggtgctggtg gacgacctca gcgagtttga cctggatgac
     5221 atgaaagccg agggccggga gcctgccctg gtagtggaaa ccagcccgaa gaactatcag
     5281 gcatgggtca aggtggccga cgccgcaggc ggtgaacttc gggggcagat tgcccggacg
     5341 ctggccagcg agtacgacgc cgacccggcc agcgccgaca gccgccacta tggccgcttg
     5401 gcgggcttca ccaaccgcaa ggacaagcac accacccgcg ccggttatca gccgtgggtg
     5461 ctgctgcgtg aatccaaggg caagaccgcc accgctggcc cggcgctggt gcagcaggct
     5521 ggccagcaga tcgagcaggc ccagcggcag caggagaagg cccgcaggct ggccagcctc
     5581 gaactgcccg agcggcagct tagccgccac cggcgcacgg cgctggacga gtaccgcagc
     5641 gagatggccg ggctggtcaa gcgcttcggt catgacctca gcaagtgcga ctttatcgcc
     5701 gcgcagaagc tggccagccg gggccgcagt gccgaggaaa tcggcaaggc catggccgag
     5761 gccagcccag cgctggcaga gcgcaagccc ggccacgaag cggattacat cgagcgcacc
     5821 gtcagcaagg tcatgggtct gcccagcgtc cagcttgcgc gggccgagct ggcacgggca
     5881 ccggcacccc gccagcgagg catggacagg ggcgggccag atttcagcat gtagtgcttg
     5941 cgttggtact cacgcctgtt atactatgag tactcacgca cagaaggggg ttttatggaa
     6001 tacgaaaaaa gcgcttcagg gtcggtctac ctgatcaaaa gtgacaaggg ctattggttg
     6061 cccggtggct ttggttatac gtcaaacaag gccgaggctg gccgcttttc agtcgctgat
     6121 atggccagcc ttaaccttga cggctgcacc ttgtccttgt tccgcgaaga caagcctttc
     6181 ggccccggca agtttctcgg tgactgatat gaaagaccaa aaggacaagc agaccggcga
     6241 cctgctggcc agccctgacg ctgtacgcca agcgcgatat gccgagcgca tgaaggccaa
     6301 agggatgcgt cagcgcaagt tctggctgac cgacgacgaa tacgaggcgc tgcgcgagtg
     6361 cctggaagaa ctcagagcgg cgcagggcgg gggtagtgac cccgccagcg cctaaccacc
     6421 aactgcctgc aaaggaggca atcaatggct acccataagc ctatcaatat tctggaggcg
     6481 ttcgcagcag cgccgccacc gctggactac gttttgccca acatggtggc cggtacggtc
     6541 ggggcgctgg tgtcgcccgg tggtgccggt aaatccatgc tggccctgca actggccgca
     6601 cagattgcag gcgggccgga tctgctggag gtgggcgaac tgcccaccgg cccggtgatc
     6661 tacctgcccg ccgaagaccc gcccaccgcc attcatcacc gcctgcacgc ccttggggcg
     6721 cacctcagcg ccgaggaacg gcaagccgtg gctgacggcc tgctgatcca gccgctgatc
     6781 ggcagcctgc ccaacatcat ggccccggag tggttcgacg gcctcaagcg cgccgccgag
     6841 ggccgccgcc tgatggtgct ggacacgctg cgccggttcc acatcgagga agaaaacgcc
     6901 agcggcccca tggcccaggt catcggtcgc atggaggcca tcgccgccga taccgggtgc
     6961 tctatcgtgt tcctgcacca tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag
     7021 cagcaggcca gccggggcag ctcggtactg gtcgataaca tccgctggca gtcctacctg
     7081 tcgagcatga ccagcgccga ggccgaggaa tggggtgtgg acgacgacca gcgccggttc
     7141 ttcgtccgct tcggtgtgag caaggccaac tatggcgcac cgttcgctga tcggtggttc
     7201 aggcggcatg acggcggggt gctcaagccc gccgtgctgg agaggcagcg caagagcaag
     7261 ggggtgcccc gtggtgaagc ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc
     7321 ggcgcactgt ctggcccccg gcctgttccg tgccctcaag cggggcgagc gcaagcgcag
     7381 caagctggac gtgacgtatg actacggcga cggcaagcgg atcgagttca gcggcccgga
     7441 gccgctgggc gctgatgatc tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa
     7501 tggcctagtg cttggcccgg aacccaagac cgaaggcgga cggcagctcc ggctgttcct
     7561 ggaacccaag tgggaggccg tcaccgctga atgccatgtg gtcaaaggta gctatcgggc
     7621 gctggcaaag gaaatcgggg cagaggtcga tagtggtggg gcgctcaagc acatacagga
     7681 ctgcatcgag cgcctttgga aggtatccat catcgcccag aatggccgca agcggcaggg
     7741 gtttcggctg ctgtcggagt acgccagcga cgaggcggac gggcgcctgt acgtggccct
     7801 gaaccccttg atcgcgcagg ccgtcatggg tggcggccag catgtgcgca tcagcatgga
     7861 cgaggtgcgg gcgctggaca gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg
     7921 gatcgacccc ggcaaaaccg gcaaggcttc catagatacc ttgtgcggct atgtctggcc
     7981 gtcagaggcc agtggttcga ccatgcgcaa gcgccgcaag cgggtgcgcg aggcgttgcc
     8041 ggagctggtc gcgctgggct ggacggtaac cgagttcgcg gcgggcaagt acgacatcac
     8101 ccggcccaag gcggcaggct gacccccccc actctattgt aaacaagaca tttttatctt
     8161 ttatattcaa tggcttattt tcctgctaat tggtaatacc atgaaaaata ccatgctcag
     8221 aaaaggctta acaatatttt gaaaaattgc ctactgagcg ctgccgcaca gctccatagg
     8281 ccgctttcct ggctttgctt ccagatgtat gctcttctgc tcccgaacgc cagcaagacg
     8341 tagcccagcg cgtcggccag cttgcaattc gcgctaactt acattaattg cgttgcgctc
     8401 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8461 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     8521 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     8581 cgtggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca
     8641 tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc
     8701 ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc
     8761 agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact
     8821 ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca
     8881 gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg
     8941 ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa
     9001 aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt
     9061 gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc
     9121 actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg
     9181 ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc
     9241 gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga
     9301 ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc
     9361 cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga
     9421 aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac
     9481 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
     9541 gcaccattcg atggtgtcaa cgtaaatgcc gcttcgcctt cgcgcgcgaa ttgcaagctg
     9601 atccgggctt atcgactgca cggtgcacca atgcttctgg cgtcaggcag ccatcggaag
     9661 ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact
     9721 cccgttctgg ataatgtttt ttgcgccgac atcataacgg ttctggcaaa tattctgaaa
     9781 tgagc
//

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