| 质粒名称: | RhGC(BE)_pGEM |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 85469 |
| 存储实验室: | Peter Hegemann |
|
载体骨架基本信息 |
|
| 载体名称: | pGEM |
| 载体出品公司: | Clontech |
| 空载体大小: | 3045bp |
| 完整质粒大小: | 4923bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | rhodopsin-guanylyl cyclase/RhGC(BE)/RhGC |
| 物种来源: | Synthetic; Blastocladiella emersonii |
| 插入大小: | 1878bp |
| GenBank编号 | KP731361.1 |
| 启动子: | T7 Promoter |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI (unknown if destroyed) |
| 3’克隆位点: | HindIII (unknown if destroyed) |
| 5’测序引物 | T7 forward |
| 3’测序引物 |
Seq12rv: GTGTAAGTTGGTATTATGTAG
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: | RhGC(BE)_pGEM was a gift from Peter Hegemann (Addgene plasmid # 85469 ; http://n2t.net/addgene:85469 ; RRID:Addgene_85469) |
| 参考文献部分: | The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling. Scheib U, Stehfest K, Gee CE, Korschen HG, Fudim R, Oertner TG, Hegemann P. Sci Signal. 2015 Aug 11;8(389):rs8. doi: 10.1126/scisignal.aab0611. 10.1126/scisignal.aab0611 PubMed 26268609 |
买家导航
RhGC(BE)_pGEM载体质粒基本信息
RhGC(BE)_pGEM质粒图谱载体图谱和RhGC(BE)_pGEM载体序列质粒序列多克隆位点信息
_pGEM质粒图谱.png)
RhGC(BE)_pGEM质粒载体简介
RhGC(BE)_pGEM质粒序列载体序列
LOCUS RhGC(BE)_pGEM 4923 bp DNA circular SYN 25-AUG-2024
DEFINITION The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella
emersonii enables fast optical control of cGMP signaling.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4923)
AUTHORS Scheib U, Stehfest K, Gee CE, Korschen HG, Fudim R, Oertner TG,
Hegemann P
TITLE The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella
emersonii enables fast optical control of cGMP signaling.
JOURNAL Sci Signal. 2015 Aug 11;8(389):rs8. doi: 10.1126/scisignal.aab0611.
PUBMED 26268609
REFERENCE 2 (bases 1 to 4923)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Sci
Signal."; date: "2015-08-11"; volume: "8(389):rs8. doi"; pages: "
10.1126/scisignal.aab0611"
FEATURES Location/Qualifiers
source 1..4923
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 33..55
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 47..64
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 48..64
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 71..90
/label=T7
/note="T7 promoter, forward primer"
promoter 71..89
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
5'UTR 121..163
/label=Xenopus globin 5'-UTR
/note="translational enhancer from the 5'-UTR of the major
beta-globin gene of Xenopus laevis"
3'UTR 2089..2214
/label=Xenopus globin 3'-UTR
/note="3'-UTR of the major beta-globin gene of Xenopus
laevis"
promoter complement(2335..2353)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(2336..2353)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(2371..2387)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2371..2387)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(2384..2406)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 2395..2411
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2419..2449)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2464..2485
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(2602..2619)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2773..3361)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2853..2872)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3532..4392)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4155..4174
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4393..4497)
/gene="bla"
/label=AmpR promoter
primer_bind 4565..4583
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(4621..4643)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 4743..4762
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
61 cagtgaattg taatacgact cactataggg cgaattaatt cgagctcggt acccagcttg
121 cttgttcttt ttgcagaagc tcagaataaa cgctcaactt tggcagatca attccccggg
181 gatccatgaa agataaggat aataacctga gaggagcttg ttctagctgt aactgccctg
241 aatactgttt tagcccaact tccaccctgt gcgacgattg caagtgttcc gtgaccaaac
301 acccaatcgt cgaacagccc ctgtcccgaa acggatcttt ccggagctcc ggagcaagtc
361 tgctgccatc accttctagt cccaatgtga agattacctc tacagtcggc ctgcggagta
421 gaaaaagcga gtcccaggca aacgtgcgcg ggtctatgat ctctaacagt aattcaggaa
481 gccgatccaa caatagtgga ggagcaggag gagggtcagg aggctcaagc tcctctaagg
541 ggggcagcgc cctggctaac taccagtccg ctatgtctga gctgtggtct tggaatatga
601 tgctgagtac tccttcactg aaattcctga ccgtgcagtt taccacatgg attgtgctga
661 ctaccgtcgg cgccatctac actctgttct ttcatgaaag acaggcttat aatagggggt
721 gggcagacat ctggtacggc tatggggctt tcggatttgg cctggggctg agcttcgcat
781 atatgggctt caccggcgcc agaaacccag agaagaaagc tctgagcctg tgcctgctgg
841 gggtgaattt cattagcttt atgtcctaca tcatcatcat gctgcggctg actcccacca
901 tcgaaggcac catggccaac ccagtggagc ccgctagata tctggaatgg atcgccactt
961 gccctgtcct gatcctgctg attagcgaga tcacccagta ccctcacgac ccatataaag
1021 tgatcgtcaa tgattacgcc ctgtgcctgg caggattcgt gggagcaatc agcgcccagc
1081 agccatgggg cgacctggca catttcgtga gctgcctgtg cttttcctac gtggtctatt
1141 ctctgtggag ttgcttcaca ggggctatcg atggagagac tcagtgtaac gtggaaaaaa
1201 gcggcctgag gtggattcgc ttttcaacaa tcacaacttg gagcctgttc cccattacct
1261 ggttttcata tacaagcggc ctgatctctt tcactgtggc agaggccggg tttagtatga
1321 ttgacatcgg agccaaggtc ttcctgaccc tggtgctggt caacagcaca gtggaacagg
1381 cacagaatca gaaagtcgac gccattaccg ctatcgcaga ggaactggag aaccagatta
1441 acaattgtga tgccatcctg cagaagatga tgcccgaggg cgtgctggaa cagctgaaga
1501 atgggcaggc tactgaggca aaagagtacg aatccgtgac cgtcttcttt tctgatatta
1561 ctaactttac cgtgatcagt tcaaggacat ccactaagga catgatggcc accctgaaca
1621 agctgtggct ggaatatgat gccattgcta agcggtgggg agtgtataaa gtcgagacaa
1681 tcggcgacgc ttatctggga gtgactggag caccagacgt ggtccctgat cacgcagagc
1741 gagcttgcaa cttcgccgtg gatatcatcg aaatgattaa gagctttaaa accatcacag
1801 gcgagtccat taacatccgc attggcctga atagcggacc tgtgaccgca ggagtcctgg
1861 gcgacctgaa cccacactgg tgtctggtgg gagatacagt caatactgcc tcccggatgg
1921 aatctacaag taaagctggc cacatccata tttcagagag cacctaccat ttcatcaagt
1981 ctaaatttgt gacacagcct ctggatgtga tggaggtgaa aggcaaaggc aaaatgcaga
2041 cttattgggt gctgggcaga aaatagaagc ttgatctggt taccactaaa ccagcctcaa
2101 gaacacccga atggagtctc taagctacat aataccaact tacactttac aaaatgttgt
2161 cccccaaaat gtagccattc gtatctgctc ctaataaaaa gaaagtttct tcacattcta
2221 aaaaaaaaaa aaaaaaaaaa aacccccccc cccccccccc cccccccccc ccctgcaggc
2281 atgcgtttaa acatttaaat tacgtattaa ttaagatatc gctagcttga gtattctata
2341 gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
2401 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
2461 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
2521 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
2581 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
2641 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
2701 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
2761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
2821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
2881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
2941 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
3001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
3061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
3121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
3181 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
3241 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
3301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
3361 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
3421 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
3481 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
3541 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
3601 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
3661 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
3721 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
3781 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
3841 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
3901 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
3961 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
4021 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
4081 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
4141 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
4201 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
4261 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
4321 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
4381 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
4441 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
4501 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
4561 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
4621 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
4681 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
4741 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
4801 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
4861 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
4921 tgc
//
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