| 质粒名称: | pJYS2cm_crtYf |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 85543 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pXMJ19 and pTRCmob |
| 空载体大小: | 4038 bp |
| 完整质粒大小: | 4064bp |
| 载体类型: | Bacterial Expression, CRISPR |
| 载体抗性: | Chloramphenicol |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | crRNA of crtYf |
| 物种来源: | Synthetic |
|
文献引用方法 |
|
| 材料和方法部分: | pJYS2cm_crtYf was a gift from Sheng Yang (Addgene plasmid # 85543 ; http://n2t.net/addgene:85543 ; RRID:Addgene_85543) |
| 参考文献部分: | CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, Li Y, Wang R, Yang S. Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179. 10.1038/ncomms15179 PubMed 28469274 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJYS2cm_crtYf 4064 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Constitutive transcription of crRNA of crtYf, Chloramphenicol
resistance.
ACCESSION .
VERSION .
KEYWORDS pJYS2cm_crtYf
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4064)
AUTHORS Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X,
Li Y, Wang R, Yang S
TITLE CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
JOURNAL Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
PUBMED 28469274
REFERENCE 2 (bases 1 to 4064)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4064
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 77..665
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 566..585
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 819..836
/label=L4440
/note="L4440 vector, forward primer"
terminator 930..957
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 1049..1134
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(3245..3904)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 3819..3838
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
781 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
841 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
901 tatgaagctt aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
961 taatttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
1021 tcgcaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
1081 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttaaattt
1141 cctgccctat ggttgcctga tctacaacag tagaaattcg gatccattat acctaggact
1201 gagctagctg tcaatctaga ctcgaggaga tttttgaggg ggagggagtc gaggaagagc
1261 cagagcagaa ggcgggggaa ccgttctctg ccgacagcgt gagccccctt aaaaatcagg
1321 ccggggagga accggggagg gatcagagct aggagcgaga caccctaaag ggggggaacc
1381 gttttctgct gacggtgttt cgtttattag ttttcagccc gtggatagcg gagggtgagg
1441 gcaagtgaga gccagagcaa ggacgggacc cctaaagggg ggaaccgttt tctgctgacg
1501 gtgtttcgtt tattagtttt cagcccgtgg acggccgcgt ttagcttcca ttccaagtgc
1561 ctttctgact tgttggatgc gcctttcact gacacctagt tcgcctgcaa gctcacgagt
1621 cgagggatca gcaaccgatt gagaacgggc atccaggatc gcagttttga cgcgaagttc
1681 gagcaactcg cctgtcattt ctcggcgttt gtttgcttcc gctaatcgct gtcgcgtctc
1741 ctgcgcatac ttactttctg ggtcagccca tctgcgtgca ttcgatgtag ctgcgccccg
1801 tcgccccatc gtcgctagag ctttccgccc tcggctgctc tgcgtttcca cccgacgagc
1861 agggacgact ggctggcctt tagccacgta gccgcgcaca cgacgcgcca tcgtcaggcg
1921 atcacgcatg gcgggaagat ccggctcccg gccgtctgca ccgaccgcct gggcaacgtt
1981 gtacgccact tcatacgcgt cgatgatctt ggcatctttt aggcgctcac cagcagcttt
2041 gagctggtat cccacggtca acgcgtggcg aaacgcggtc tcgtcgcgcg ctgcgcgctc
2101 tggatttgtc cagagcactc gcacgccgtc gatcaggtcg ccggacgcgt ccagggcgct
2161 cggcaggctc gcgtccaaaa tcgctagcgc cttggcttct gcggtggcgc gttgtgccgc
2221 ttcaatgcgg gcgcgtccgc tggaaaagtc ctgctcaatg tactttttcg gcttctgtga
2281 tccggtcatc gttcgagcaa tctccattag gtcggccagc cgatccacac gatcatgctg
2341 gcagtgccat ttataggctg tcggatcgtc tgagacgtgc agcggccacc ggctcagcct
2401 atgcgaaaaa gcctggtcag cgccgaaaac acgagtcatt tcttccgtcg ttgcagccag
2461 caggcgcata tttgggctgg ttttacctgc tgcggcatac accgggtcaa tgagccagat
2521 gagctggcat ttcccgctca gcggattcac gccgatccaa gccggcgctt tttctaggcg
2581 tgcccatttc tctaaaatcg cgtagacctg cgggtttacg tgctcaatct tcccgccggc
2641 ctggtggctg ggcacatcga tgtcaagcac gatcaccgcg gcatgttgcg cgtgcgtcag
2701 cgcaacgtac tggcaccgcg tcagcgcttt tgagccagcc cggtagagct ttggttgggt
2761 ttcgccggta tccgggtttt taatccaggc gctcgcgaaa tctcttgtct tgctgccctg
2821 gaagctttcg cgtcccaggt gagcgagcag ttcgcggcga tcttctgccg tccagccgcg
2881 tgagcgcagc gctagttcgg ggtgggtgtc gaacagatcg gcggaaaatt tccacgcgct
2941 agctgtgact gtgtcctgcg gatcggctag agtcatgtct tgagtgcttt ctcccagctg
3001 atgactgggg gttagccgac gccctgtgag ttcccgctca cggggcgttc aactttttca
3061 ggtatttgtg cagcttatcg tgttttcttc gtaaatgaac gcttaactac cttgttaaac
3121 gtggcaaata ggcaggattg atggctttca tagaaggcgg cggtggaatc gaaatctcgt
3181 gatggcaggt tgggcgtcgc ttggtcggtc atttcgaagg gcaccaataa ctgccttaaa
3241 aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
3301 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
3361 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
3421 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
3481 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
3541 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
3601 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
3661 gctcaccgtc tttcattgcc atacgtaact ccggatgagc attcatcagg cgggcaagaa
3721 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
3781 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
3841 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
3901 ccattttagc ttccttagct cctgaaaatc tcgtcgaagc tcggcggatt tgtcctactc
3961 aagctgatcc gacaaaatcc acacattatc acaggtgtcc ggatcggtca aatacgctgc
4021 cagctcatag accgtatcca aagcatccgg ggctgatccc cggc
//
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