pLSLR

LOCUS       pLSLR               10531 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  Single component plant T-DNA plasmid. Contains cis- and trans-acting
            replicational elements from the bean yellow dwarf virus in an 
            LIR-SIR-Rep/RepA-LIR orientation..
ACCESSION   .
VERSION     .
KEYWORDS    pLSLR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan 17.
  PUBMED    24443519
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pLSLR|
FEATURES             Location/Qualifiers
     source          1..10531
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1129..1323
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1559..1581)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1667..1807
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1722..1741
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1822..1839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1993..2581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2073..2092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2668..3462)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    3887..3911
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    3989..4163
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(5253..5930)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    8140..8164
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9464..10093
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10527..1063
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
ORIGIN
        1 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
       61 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
      121 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
      181 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
      241 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
      301 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
      361 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
      421 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
      481 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
      541 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
      601 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
      661 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
      721 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
      781 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
      841 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
      901 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
      961 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     1021 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     1081 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac
     1141 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     1201 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     1261 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     1321 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     1381 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     1441 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     1501 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     1561 tctgacacat gcagctcccg gaaacggtca cagcttgtct gtaagcggat gccgggagca
     1621 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     1681 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     1741 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     1801 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2401 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2641 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
     2701 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     2761 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
     2821 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
     2881 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
     2941 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
     3001 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
     3061 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
     3121 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
     3181 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
     3241 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
     3301 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
     3361 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
     3421 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
     3481 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
     3541 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
     3601 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
     3661 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
     3721 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
     3781 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
     3841 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
     3901 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
     3961 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
     4021 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
     4081 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
     4141 actcacacat tattatggag aaaatagagc ttgtcgatcg ttaattaatc acctccaaga
     4201 tcccggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
     4261 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
     4321 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
     4381 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga
     4441 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
     4501 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
     4561 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
     4621 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
     4681 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
     4741 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
     4801 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
     4861 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
     4921 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc
     4981 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
     5041 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
     5101 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaca cgctgtcgaa
     5161 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatcctagg
     5221 agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa
     5281 cttccttata tagaggaagg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
     5341 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
     5401 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
     5461 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
     5521 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
     5581 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
     5641 tcttggagta gacgagagtg tcgtgctcca ccatgttatc acatcaatcc acttgctttg
     5701 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
     5761 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
     5821 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
     5881 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
     5941 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
     6001 tgtcgactat cggacgagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact
     6061 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
     6121 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
     6181 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
     6241 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
     6301 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
     6361 caggtatttc cgttggatac gaattattcg tacgaccctc ggcgcgccga gaacttccag
     6421 agctttgggt acgtcacgtg gctcgagcgc gtagtcctcg gtaatatcgc agaacaaaag
     6481 tacctgatat cgagtgtact tcaagtcagt gggaaatcaa taaaatgatt attttatgaa
     6541 tatatttcat tgtgcaagta gatagaaatt acatatgtta cataacacac gaaataaaca
     6601 aaaaaagaca atccaaaaac aaacacccca aaaaaaataa tcactttaga taaactcgta
     6661 tgaggagagg cacgttcagt gactcgacga ttcccgagca aaaaaagtct ccccgtcaca
     6721 catgtagtgg gtgacgcaat tatctttaaa gtaatccttc tgttgacttg tcattgataa
     6781 catccagtct tcgtcaggat tgcaaagaat tatagaaggg atcccacctt ttattttctt
     6841 cttttttcca tatttagggt tgacagtgaa atcagactgg caacctatta attgcttcca
     6901 caatgggacg aacttgaagg ggatgtcgtc gatgatatta taggtggcgt gttcatcgta
     6961 gttggtgaaa tcgatggtac cgttccaata gttgtgtcgt ccgagacttc tagcccaggt
     7021 ggtctttccg gtacgagttg gtccgcagat gtagaggctg gggtgtcgga ttccattcct
     7081 tccattgtcc ttgttaaatc ggccatccat tcaaggtcag attgagcttg ttggtatgag
     7141 acaggatgta tgtaagtata agcgtctatg cttacatggt atagatgggt ttccctccag
     7201 gagtgtagat cttcgtggca gcgaagatct gattctgtga agggcgacac atacggttca
     7261 ggttgtggag ggaataattt gttggctgaa tattccagcc attgaagctt tgttgcccat
     7321 tcatgaggga attcttcctt gatcatgtca agatattcct ccttagacgt tgcagtctgg
     7381 ataatagttc tccatcgtgc gtcagatttg cgaggagaaa ccttatgatc tcggaaatct
     7441 cctctggttt taatatctcc gtcctttgat atgtaatcaa ggacttgttt agagtttcta
     7501 gctggctgga tattagggtg atttccttca aaatcgaaaa aagaaggatc cctaatacaa
     7561 ggttttttat caagctggag aagagcatga tagtgggtag tgccatcttg atgaagctca
     7621 gaagcaacac caaggaagaa aataagaaaa ggtgtgagtt tctcccagag aaactggaat
     7681 aaatcatctc tttgagatga gcacttggga taggtaagga aaacatattt agattggagt
     7741 ctgaagttct tactagcaga aggcatgttg ttgtgactcc gaggggttgc ctcaaactct
     7801 atcttataac cggcgtggag gcatggaggc aggggtattt tggtcatttt aatagatagt
     7861 ggaaaatgac gtggaattta cttaaagacg aagtctttgc gacaaggggg ggcccacgcc
     7921 gaatttaata ttaccggcgt ggccccccct tatcgcgagt gctttagcac gagcggtcca
     7981 gatttaaagt agaaaatttc ccgcccacta gggttaaagg tgttcacact ataaaagcat
     8041 atacgatgtg atggtatttg atggagcgta tattgtatca ggtatttccg ttggatacga
     8101 attattcgta cgaccctcat agtttaaact atcagtgttt gacaggatat attggcgggt
     8161 aaacctaaga gaaaagagcg tttattagaa taacggatat ttaaaagggc gtgaaaaggt
     8221 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
     8281 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
     8341 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
     8401 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
     8461 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
     8521 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
     8581 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
     8641 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
     8701 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
     8761 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
     8821 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     8881 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     8941 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     9001 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     9061 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     9121 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     9181 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     9241 acgtgttcga gccgcccgcg cacggctcaa ccgtgcggct gcatgaaatc ctggccggtt
     9301 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     9361 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     9421 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     9481 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     9541 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     9601 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     9661 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     9721 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     9781 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     9841 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     9901 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     9961 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
    10021 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
    10081 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
    10141 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
    10201 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
    10261 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
    10321 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
    10381 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
    10441 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac
    10501 ccccaagccc gaggaatcgg cgtgacggtc g
//