| 质粒名称: | pCDH-EF1-mVenus-p27K |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 176651 |
| 存储实验室: | Mohamed Bentires-Alj |
|
载体骨架基本信息 |
|
| 载体名称: | pCDH |
| 载体出品公司: | System Biosciences |
| 空载体大小: | 7082bp |
| 完整质粒大小: | 8420bp |
| 载体类型: | Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | NEB Stable |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | mVenus-p27K-、Cdkn1b |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 1338bp |
| 基因突变: | Mutant K- (lacks affinity to Cdk) |
| 标签/融合蛋白: | mVenus (N terminal on backbone) |
|
文献引用方法 |
|
| 材料和方法部分: | pCDH-EF1-mVenus-p27K− was a gift from Mohamed Bentires-Alj (Addgene plasmid # 176651 ; http://n2t.net/addgene:176651 ; RRID:Addgene_176651) |
| 参考文献部分: | Hepatic stellate cells suppress NK cell-sustained breast cancer dormancy. Correia AL, Guimaraes JC, Auf der Maur P, De Silva D, Trefny MP, Okamoto R, Bruno S, Schmidt A, Mertz K, Volkmann K, Terracciano L, Zippelius A, Vetter M, Kurzeder C, Weber WP, Bentires-Alj M. Nature. 2021 Jun;594(7864):566-571. doi: 10.1038/s41586-021-03614-z. Epub 2021 Jun 2. 10.1038/s41586-021-03614-z PubMed 34079127 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCDH-EF1-mVenus-p27K− 8438 bp DNA circular SYN
25-AUG-2024
DEFINITION It encodes an mVenus fused to a mutant
p27K-, which lacks binding
affinity to Cdk. This
reporter helps to identify quiescent
disseminated
tumor cells (in G0 phase of cell cycle)..
ACCESSION .
VERSION
.
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM
synthetic DNA construct
REFERENCE 1 (bases 1 to 8438)
AUTHORS
Correia AL, Guimaraes JC, Auf der Maur P, De Silva D, Trefny MP,
Okamoto R, Bruno S, Schmidt A, Mertz K, Volkmann K,
Terracciano L,
Zippelius A, Vetter M, Kurzeder C,
Weber WP, Bentires-Alj M
TITLE Hepatic stellate cells
suppress NK cell-sustained breast cancer
dormancy.
JOURNAL Nature. 2021 Jun;594(7864):566-571. doi: 10.1038/s41586
-021-03614-z.
Epub 2021 Jun 2.
PUBMED 34079127
REFERENCE 2 (bases 1 to 8438)
AUTHORS .
TITLE Direct
Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server
7.0.3
https://www.biofeng.com
COMMENT SGRef:
number: 1; type: "Journal Article"; doi:
"10.1038/s41586-021-03614-z"; journalName: "Nature"; date:
"2021-06"; volume: "594"; issue: "7864"; pages: "566-571"
FEATURES Location/Qualifiers
source
1..8438
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 66..88
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 80..97
/label=M13 Forward
/note="In lacZ gene. Also
called M13-F20 or M13 (-21)
Forward"
primer_bind 81..97
/label=M13 fwd
/note="common sequencing primer, one of multiple
similar
variants"
promoter
112..338
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR
339..519
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-
1"
misc_feature 566..691
/label=HIV-1
Psi
/note="packaging signal of human
immunodeficiency virus
type 1"
misc_feature 1184..1417
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows
for
Rev-dependent mRNA export from the
nucleus to the
cytoplasm."
CDS
1602..1646
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje
Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the
2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 1913..2029
/label=cPPT/CTS
/note="central
polypurine tract and central termination
sequence of HIV-1"
promoter 2072..2283
/label=EF-1-alpha core promoter
/note="core
promoter for human elongation factor
EF-1-
alpha"
LTR 2296..2564
/label=5' LTR (truncated)
/note="truncated 5'
long terminal repeat (LTR) from human
T-cell
leukemia virus (HTLV) type 1"
CDS 2592..3308
/codon_start=1
/product="Venus
YFP with monomerizing A206K mutation (Nagai
et
al., 2002; Kremers et al., 2006)"
/label=mVenus
/note="mammalian codon-
optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement
(2637..2658)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement
(2898..2917)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3245..3266
/label=EGFP-C
/note="EGFP, forward primer"
CDS
3951..4004
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 4011..4610
/codon_start=1
/gene="pac
from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to
puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind
complement(4011..4030)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer.
Also
called puro-variant-R"
primer_bind
4507..4527
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature
4623..5211
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement
(4676..4696)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement
(5094..5105)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5285..5518
/label=3' LTR (Delta-U3)
/note="self-
inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5590..5711
/label=SV40 poly(A) signal
/note="SV40
polyadenylation signal"
primer_bind complement
(5627..5646)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind
5681..5700
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin
5751..5886
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement
(5919..5935)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5919..5935)
/label=M13 Reverse
/note="In
lacZ gene. Also called M13-rev"
primer_bind complement
(5932..5954)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5943..5959
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter
complement(5967..5997)
/label=lac
promoter
/note="promoter for the E. coli lac
operon"
protein_bind 6012..6033
/label=CAP binding site
/bound_moiety="E. coli
catabolite activator protein"
/note="CAP
binding activates transcription in the presence
of cAMP."
primer_bind complement(6150..6167)
/label=L4440
/note="L4440 vector,
forward primer"
rep_origin complement(6321..6909)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin
of
replication"
CDS
complement(7080..7940)
/codon_start=1
/gene="bla"
/product="beta-
lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7703..7722
/label=Amp-R
/note="Ampicillin resistance
gene, reverse primer"
promoter complement(7941..8045)
/gene="bla"
/label=AmpR
promoter
primer_bind 8113..8131
/label=pBRforEco
/note="pBR322 vectors,
upsteam of EcoRI site, forward
primer"
primer_bind complement(8169..8191)
/label=pGEX 3'
/note="pGEX vectors, reverse
primer"
primer_bind 8291..8310
/label=pRS-marker
/note="pRS vectors, use to
sequence yeast selectable
marker"
ORIGIN
1 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
taacgccagg
61 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
aagctgacgc gtgtagtctt
121 atgcaatact cttgtagtct tgcaacatgg
taacgatgag ttagcaacat gccttacaag
181 gagagaaaaa gcaccgtgca
tgccgattgg tggaagtaag gtggtacgat cgtgccttat
241 taggaaggca
acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
301
gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
361 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
caataaagct
421 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
tgactctggt aactagagat
481 ccctcagacc cttttagtca gtgtggaaaa
tctctagcag tggcgcccga acagggactt
541 gaaagcgaaa gggaaaccag
aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
601 cacggcaaga
ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
661
ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
721 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
aacatatagt
781 atgggcaagc agggagctag aacgattcgc agttaatcct
ggcctgttag aaacatcaga
841 aggctgtaga caaatactgg gacagctaca
accatccctt cagacaggat cagaagaact
901 tagatcatta tataatacag
tagcaaccct ctattgtgtg catcaaagga tagagataaa
961 agacaccaag
gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
1021
acagcaagcg gccactgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
1081 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
cccaccaagg
1141 caaagagaag agtggtgcag agagaaaaaa gagcagtggg
aataggagct ttgttccttg
1201 ggttcttggg agcagcagga agcactatgg
gcgcagcgtc aatgacgctg acggtacagg
1261 ccagacaatt attgtctggt
atagtgcagc agcagaacaa tttgctgagg gctattgagg
1321 cgcaacagca
tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
1381
tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
1441 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
tctctggaac
1501 agatttggaa tcacacgacc tggatggagt gggacagaga
aattaacaat tacacaagct
1561 taatacactc cttaattgaa gaatcgcaaa
accagcaaga aaagaatgaa caagaattat
1621 tggaattaga taaatgggca
agtttgtgga attggtttaa cataacaaat tggctgtggt
1681 atataaaatt
attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
1741
tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
1801 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
ggagagagag
1861 acagagacag atccattcga ttagtgaacg gatctcgacg
gtatcggtta acttttaaaa
1921 gaaaaggggg gattgggggg tacagtgcag
gggaaagaat agtagacata atagcaacag
1981 acatacaaac taaagaatta
caaaaacaaa ttacaaaatt caaaatttta tcgatactag
2041 tggatctgcg
atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
2101
gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
2161 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
gggagaaccg
2221 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
acgggtttgc cgccagaaca
2281 cagctgaagc ttcgaggggc tcgcatctct
ccttcacgcg cccgccgccc tacctgaggc
2341 cgccatccac gccggttgag
tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
2401 gcgtccgccg
tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
2461
ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
2521 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct
gtgaccggcg
2581 cctactctag aatggtgagc aagggcgagg agctgttcac
cggggtggtg cccatcctgg
2641 tcgagctgga cggcgacgta aacggccaca
agttcagcgt gtccggcgag ggcgagggcg
2701 atgccaccta cggcaagctg
accctgaagc tgatctgcac caccggcaag ctgcccgtgc
2761 cctggcccac
cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
2821
accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
2881 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
aagttcgagg
2941 gcgacaccct ggtgaaccgc atcgagctga agggcatcga
cttcaaggag gacggcaaca
3001 tcctggggca caagctggag tacaactaca
acagccacaa cgtctatatc accgccgaca
3061 agcagaagaa cggcatcaag
gccaacttca agatccgcca caacatcgag gacggcggcg
3121 tgcagctcgc
cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
3181
ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg
3241 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
atggacgagc
3301 tgtacaaggg atatccatca cactggcggc cgctcgaggc
caccatgtca aacgtgagag
3361 tgtctaacgg gagcccgagc ctggagcgga
tggacgccag acaagcggag caccccaagc
3421 cttccgcctg cagaaatctc
ttcggcccgg tcaatcatga agaactaacc cgggacttgg
3481 agaagcactg
ccgggatatg gaagaagcga gtcagcgcaa gtggaatgcc gacgcccaga
3541
atcataagcc cctggagggc agatacgagt ggcaggaggt ggagaggggc agcttgcccg
3601 agttctacta caggcccccg cgccccccca agagcgcctg caaggtgctg
gcgcaggaga
3661 gccaggatgt cagcgggagc cgccaggcgg tgcctttaat
tgggtctcag gcaaactctg
3721 aggaccggca tttggtggac caaatgcctg
actcgtcaga caatccggct gggttagcgg
3781 agcagtgtcc agggatgagg
aagcgacctg ctgcagaaga ttcttcttcg caaaacaaaa
3841 gggccaacag
aacagaagaa aatgtttcag acggttcccc gaacgctggc actgtggagc
3901
agacgcccaa gaagcccggc cttcgacgcc agacgggatc cgcggccgct gagggcagag
3961 gaagtcttct aacatgcggt gacgtggagg agaatcccgg cccttccggg
atgaccgagt
4021 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
cagggccgta cgcaccctcg
4081 ccgccgcgtt cgccgactac cccgccacgc
gccacaccgt cgatccggac cgccacatcg
4141 agcgggtcac cgagctgcaa
gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
4201 tgtgggtcgc
ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
4261
cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
4321 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
cccgcgtggt
4381 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
gggtctgggc agcgccgtcg
4441 tgctccccgg agtggaggcg gccgagcgcg
ccggggtgcc cgccttcctg gagacctccg
4501 cgccccgcaa cctccccttc
tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
4561 tgcccgaagg
accgcgcacc tggtgcatga cccgcaagcc cggtgcctga atctaggtcg
4621
acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
4681 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
attgcttccc
4741 gtatggcttt cattttctcc tccttgtata aatcctggtt
gctgtctctt tatgaggagt
4801 tgtggcccgt tgtcaggcaa cgtggcgtgg
tgtgcactgt gtttgctgac gcaaccccca
4861 ctggttgggg cattgccacc
acctgtcagc tcctttccgg gactttcgct ttccccctcc
4921 ctattgccac
ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
4981
tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
5041 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
ccttcggccc
5101 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
tctgcggcct cttccgcgtc
5161 ttcgccttcg ccctcagacg agtcggatct
ccctttgggc cgcctccccg cctggtacct
5221 ttaagaccaa tgacttacaa
ggcagctgta gatcttagcc actttttaaa agaaaagggg
5281 ggactggaag
ggctaattca ctcccaacga agataagatc tgctttttgc ttgtactggg
5341
tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
5401 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
tctgttgtgt
5461 gactctggta actagagatc cctcagaccc ttttagtcag
tgtggaaaat ctctagcagt
5521 agtagttcat gtcatcttat tattcagtat
ttataacttg caaagaaatg aatatcagag
5581 agtgagagga acttgtttat
tgcagcttat aatggttaca aataaagcaa tagcatcaca
5641 aatttcacaa
ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
5701
aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
5761 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
tttatttatg
5821 cagaggccga ggccgcctcg gcctctgagc tattccagaa
gtagtgagga ggcttttttg
5881 gaggcctaga cttttgcaga gaccaaattc
gtaatcatgt catagctgtt tcctgtgtga
5941 aattgttatc cgctcacaat
tccacacaac atacgagccg gaagcataaa gtgtaaagcc
6001 tggggtgcct
aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
6061
cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
6121 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
ctcggtcgtt
6181 cggctgcggc gagcggtatc agctcactca aaggcggtaa
tacggttatc cacagaatca
6241 ggggataacg caggaaagaa catgtgagca
aaaggccagc aaaaggccag gaaccgtaaa
6301 aaggccgcgt tgctggcgtt
tttccatagg ctccgccccc ctgacgagca tcacaaaaat
6361 cgacgctcaa
gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
6421
cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
6481 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
gtatctcagt
6541 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
aaccccccgt tcagcccgac
6601 cgctgcgcct tatccggtaa ctatcgtctt
gagtccaacc cggtaagaca cgacttatcg
6661 ccactggcag cagccactgg
taacaggatt agcagagcga ggtatgtagg cggtgctaca
6721 gagttcttga
agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
6781
gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
6841 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
cagaaaaaaa
6901 ggatctcaag aagatccttt gatcttttct acggggtctg
acgctcagtg gaacgaaaac
6961 tcacgttaag ggattttggt catgagatta
tcaaaaagga tcttcaccta gatcctttta
7021 aattaaaaat gaagttttaa
atcaatctaa agtatatatg agtaaacttg gtctgacagt
7081 taccaatgct
taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
7141
gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
7201 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
agcaataaac
7261 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
ctttatccgc ctccatccag
7321 tctattaatt gttgccggga agctagagta
agtagttcgc cagttaatag tttgcgcaac
7381 gttgttgcca ttgctacagg
catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
7441 agctccggtt
cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
7501
gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
7561 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
atgcttttct
7621 gtgactggtg agtactcaac caagtcattc tgagaatagt
gtatgcggcg accgagttgc
7681 tcttgcccgg cgtcaatacg ggataatacc
gcgccacata gcagaacttt aaaagtgctc
7741 atcattggaa aacgttcttc
ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
7801 agttcgatgt
aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
7861
gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
7921 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
ttatcagggt
7981 tattgtctca tgagcggata catatttgaa tgtatttaga
aaaataaaca aataggggtt
8041 ccgcgcacat ttccccgaaa agtgccacct
gacgtctaag aaaccattat tatcatgaca
8101 ttaacctata aaaataggcg
tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
8161 ggtgaaaacc
tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
8221
gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
8281 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
gtgtgaaata
8341 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
attcgccatt caggctgcgc
8401 aactgttggg aagggcgatc ggtgcgggcc
tcttcgct
//
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