| 质粒名称: | pMG36E |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 133853 |
| 存储实验室: | Jan Kok, Gerard Venema |
|
载体骨架基本信息 |
|
| 载体名称: | pWV01 |
| 载体出品公司: | Clontech |
| 空载体大小: | 1800 bp |
| 完整质粒大小: | 3600 bp |
| 载体类型: | Bacterial Expression |
| 载体抗性: | Erythromycin |
| 生长温度: | 30 ℃ |
| 宿主菌: | E.coli MC100 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 基因突变: | EmR; mcs; P32 |
| 克隆方法: | 限制性内切酶 |
|
文献引用方法 |
|
| 材料和方法部分: |
pMG36E was a gift from Jan Kok & Gerard Venema (Addgene plasmid # 133853 ; http://n2t.net/addgene:133853 ; RRID:Addgene_133853)
|
| 参考文献部分: | Construction of a lactococcal expression vector: expression of hen egg white lysozyme in Lactococcus lactis subsp. lactis. van de Guchte M, van der Vossen JM, Kok J, Venema G. Appl Environ Microbiol. 1989 Jan;55(1):224-8. PubMed 2495760 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
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载体序列
LOCUS pMG36E 3600 bp ds-DNA circular SYN 22-AUG-2022
DEFINITION Expression vector.
ACCESSION .
VERSION .
KEYWORDS pMG36E
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3600)
AUTHORS van de Guchte M, van der Vossen JM, Kok J, Venema G
TITLE Construction of a lactococcal expression vector: expression of hen
egg white lysozyme in Lactococcus lactis subsp. lactis.
JOURNAL Appl Environ Microbiol. 1989 Jan;55(1):224-8.
PUBMED 2495760
REFERENCE 2 (bases 1 to 3600)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 22, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3600
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(190..201)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
ORIGIN
1 tcgatttttt attaaaacgt ctcaaaatcg tttctgagac gttttagcgt ttatttcgtt
61 tagttatcgg cataatcgtt aaaacaggcg ttatcgtagc gtaaaagccc ttgagcgtag
121 cgtggctttg caggaagatg ttgtctgtta gattatgaaa gccgatgact gaatgaaata
181 ataagcgcag cgcccttcta tttcggttgg aggaggctca agggagtatg agggaatgaa
241 attccctcat gggtttgatt ttaaaaattg cttgcaattt tgccgagcgg tagcgctgga
301 aaatttttga aaaaaatttg gaatttggaa aaaaatgggg ggaaaggaag cgaattttgc
361 ttccgtacta cgacccccca ttaagtgccg agtgccaatt tttgtgccaa aaacgctcta
421 tcccaactgg ctcaagggtt taaggggttt ttcaatcgcc aacgaatcgc caacgttttc
481 gccaacgttt tttataaatc tatatttaag tagctttatt gttgttttta tgattacaaa
541 gcgatacact aactttataa aattatttga ttggagtttt ttaaatggtg atttcagaat
601 cgaaaaaaag agttatgatt tctctgacaa aagagcaaga taaaaaatta acagatatgg
661 cgaaacaaaa aggtttttca aaatctgcgg cgttagctat agaagaatat gcaagaaagg
721 aatcagaaca aaaaaaataa gcgaaagctc gcgtttttag aaggatacga gttttcgcta
781 cttgtttttg ataaggtaat tatatcatgg ctattaaaaa tactaaagct agaaattttg
841 gatttttatt atatcctgac tcaattccta atgattggaa agaaaaatta gagagtttgg
901 gcgtatctat ggctgtcagt cctttacacg atatggacga aaaaaaagat aaagatacat
961 ggaatagtag tgatgttata cgaaatggaa agcactataa aaaaccacac tatcacgtta
1021 tatatattgc acgaaatcct gtaacaatag aaagcgttag gaacaagatt aagcgaaaat
1081 tggggaatag ttcagttgct catgttgaga tacttgatta tatcaaaggt tcatatgaat
1141 atttgactca tgaatcaaag gacgctattg ctaagaataa acatatatac gacaaaaaag
1201 atattttgaa cattaatgat tttgatattg actgctatat aacacttgat gaaagccaaa
1261 aaagagaatt gaagaattta cttttagata tagtggatga ctataatttg gtaaatacaa
1321 aagatttaat ggcttttatt cgccttaggg gagcggagtt tggaatttta aatacgaatg
1381 atgtaaaaga tattgtttca acaaactcta gcgcctttag attatggttt gagggcaatt
1441 atcagtgtgg atatagagca agttatgcaa aggttcttga tgctgaaacg ggggaaataa
1501 aatgacaaac aaagaaaaag agttatttgc tgaaaatgag gaattaaaaa aagaaattaa
1561 ggacttaaaa gagcgtattg aaagatacag agaaatggaa gttgaattaa gtacaacaat
1621 agatttattg agaggaggga ttattgaata aataaaagcc ccctgacgaa agtcgaaggg
1681 ggtttttatt ttggtttgat gttgcgatta atagcaatac aattgcaata aacaaaatga
1741 tcgaccggac ccctatctag cgaactttta gaaaagatat aaaacatcag agtatggaca
1801 gttgcggatg tacttcagaa aagattagat gtctaaaaag ctagcttttt agacatctaa
1861 atctaggtac taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg
1921 cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga tcgacccgat
1981 tcacaaaaaa taggcacacg aaaaacaagt taagggatgc agtttatgca tcccttaact
2041 tacttattaa ataatttata gctattgaaa agagataaga attgttcaaa gctaatattg
2101 tttaaatcgt caattcctgc atgttttaag gaattgttaa attgattttt tgtaaatatt
2161 ttcttgtatt ctttgttaac ccatttcata acgaaataat tatacttttg tttatctttg
2221 tgtgatattc ttgatttttt tctacttaat ctgataagtg agctattcac tttaggttta
2281 ggatgaaaat attctcttgg aaccatactt aatatagaaa tatcaacttc tgccattaaa
2341 agtaatgcca atgagcgttt tgtatttaat aatcttttag caaacccgta ttccacgatt
2401 aaataaatct cattagctat actatcaaaa acaattttgc gtattatatc cgtacttatg
2461 ttataaggta tattaccata tattttatag gattggtttt taggaaattt aaactgcaat
2521 atatccttgt ttaaaacttg gaaattatcg tgatcaacaa gtttattttc tgtagttttg
2581 cataatttat ggtctatttc aatggcagtt acgaaattac acctctttac taattcaagg
2641 gtaaaatggc cttttcctga gccgatttca aagatattat catgttcatt taatcttata
2701 tttgtcatta ttttatctat attatgtttt gaagtaataa agttttgact gtgttttata
2761 tttttctcgt tcattataac cctctttaat ttggttatat gaattttgct tattaacgat
2821 tcattataac cacttatttt ttgtttggtt gataatgaac tgtgctgatt acaaaaatac
2881 taaaaatgcc catatttttt cctccttata aaattagtat aattatagca cggtcgatct
2941 tctatataaa agatatatta tcttatcagt attgtcaata tattcaaggc aatctgcctc
3001 ctcatcctct tcatcctctt cgtcttggta gctttttaaa tatgggtcga tcgaattcgg
3061 tcctcgggat atgataagat taatagtttt agctattaat ctttttttat ttttatttaa
3121 gaatggctta ataaagcggt tactttggat ttttgtgagc ttggactaga aaaaaacttc
3181 acaaaatgct atactaggta ggtaaaaaaa tattcggagg aattttgaaa tggcaatcgt
3241 ttcagcagaa aaattcgtaa ttcgagctcg cccggggatc gatcctctag agtcgacctg
3301 caggcatgca agctttgcaa agtctgaaaa cgaaggtggc agctgccgtt gaagcggcca
3361 agacagttgg taaaggcgac ggtacaaccg gtactagcga caaaggcggc ggtcaaggta
3421 ccccggcgct acgatatttg gagttgaggt tcaaagtcaa atggtactga tgaccggtaa
3481 aatttaatat tttgaacctt gcttaggcag ctgacttcac attgttgaga tcagctgcct
3541 tttgcttata gttcattgag tagaaacggt tctgttgcga agtttgaaaa tcaaacaagc
//
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