| 质粒名称: | AbVec2.0-IGHG3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 99577 |
| 存储实验室: | Hedda Wardemann |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 |
| 载体出品公司: | Clontech |
| 空载体大小: | 5882 bp |
| 完整质粒大小: | 5882 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 启动子: | hCMV IE1 gene promoter |
| 克隆方法: | 限制性内切酶 |
| 5’测序引物 | GCTTCGTTAGAACGCGGCTAC |
|
文献引用方法 |
|
| 材料和方法部分: | AbVec2.0-IGHG3 was a gift from Hedda Wardemann (Addgene plasmid # 99577 ; http://n2t.net/addgene:99577 ; RRID:Addgene_99577) |
买家导航
AbVec2.0-IGHG3载体质粒基本信息
AbVec2.0-IGHG3质粒图谱载体图谱和AbVec2.0-IGHG3载体序列质粒序列多克隆位点信息
AbVec2.0-IGHG3质粒载体简介
AbVec2.0-IGHG3质粒序列载体序列
LOCUS AbVec2.0-IGHG3 5882 bp DNA circular SYN 25-AUG-2024
DEFINITION Expression of secretory immunoglobulin heavy chains in mammalian
cells, human IgG3 isotype.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5882)
TITLE Immunoglobulin expression vectors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5882)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..5882
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 99..120
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 135..165
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 173..189
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 178..200
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 197..213
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 197..213
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
enhancer 242..621
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 622..825
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 776..796
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 822..846
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter 1056..1074
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 1056..1073
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind 1087..1106
/label=pMT2-F
/note="Synthetic intron, forward primer"
regulatory 2410..2419
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
polyA_signal 2423..2557
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2460..2479)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2514..2533
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
promoter 2626..2983
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2626..2817
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2649..2669)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2834..2969
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(3003..3020)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(3003..3019)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3012..3034)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 3232..3687
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3319..3338)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3529..3550
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3704..3723)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3823..3845
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(3883..3901)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 3969..4073
/gene="bla"
/label=AmpR promoter
CDS 4074..4934
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4292..4311)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5105..5693
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5594..5613
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5847..5864
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatccaactg gcacgacagg
61 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
121 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
181 ggataacaat ttcacacagg aaacagctat gacatgatta cgaattaatt cgagctcgcc
241 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
301 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
361 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
421 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
481 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
541 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
601 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
661 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
721 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
781 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
841 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
901 tccagcctcc gcggccggga acggtgcatt ggaacgcgga ttccccgtgc caagagtgac
961 gtaagtaccg cctatagagt ctataggccc acccccttgg cttcgttaga acgcggctac
1021 aattaataca taaccttatg tatcatacac atacgattta ggtgacacta tagaataaca
1081 tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcac ctcggttcta
1141 tcgattgaat tccaccatgg gatggtcatg tatcatcctt tttctagtag caactgcaac
1201 cggttgacta actagctggt cgaccaaggg cccaagcgtc ttccccctgg cgccctgctc
1261 caggagcacc tctgggggca cagcggccct gggctgcctg gtcaaggact acttcccaga
1321 gccggtgacg gtgtcgtgga actcaggcgc cctgaccagc ggcgtgcaca ccttcccggc
1381 tgtcctacag tcctcaggac tctactccct cagcagcgtg gtgaccgtgc cctccagcag
1441 cttgggcacc cagacctaca cctgcaacgt gaatcacaag cccagcaaca ccaaggtgga
1501 caagagagtt gagctgaaaa caccactggg cgatacaaca cacacttgcc caagatgccc
1561 tgaaccaaaa tcttgcgaca cacctccgcc ttgtccacga tgtcctgagc caaagagttg
1621 cgatacccct cctccttgtc cccggtgtcc cgaacccaag tcctgtgata cgcctccacc
1681 atgtcccaga tgtccagcac ctgaactcct gggaggaccg tcagtcttcc tcttcccccc
1741 aaaacccaag gataccctta tgatttcccg gacccctgag gtcacgtgcg tggtggtgga
1801 cgtgagccac gaagaccccg aggtccagtt caagtggtac gtggacggcg tggaggtgca
1861 taatgccaag acaaagccgc gggaggagca gtacaacagc acgttccgtg tggtcagcgt
1921 cctcaccgtc ctgcaccagg actggctgaa cggcaaggag tacaagtgca aggtctccaa
1981 caaagccctc ccagccccca tcgagaaaac catctccaaa accaaaggac agccccgaga
2041 accacaggtg tacaccctgc ccccatcccg ggaggagatg accaagaacc aggtcagcct
2101 gacctgcctg gtcaaaggct tctaccccag cgacatcgcc gtggagtggg agagcagcgg
2161 gcagccggag aacaactaca acaccacgcc tcccatgctg gactccgacg gctccttctt
2221 cctctacagc aagctcaccg tggacaagag caggtggcag caggggaaca tcttctcatg
2281 ctccgtgatg catgaggctc tgcacaaccg cttcacgcag aagagcctct ccctgtctcc
2341 gggtaaatga gtgcgacggc cggcaagccc ccgctccccg ggctctcggg gtcgcgcgag
2401 gaaagcttgg ccgccatggc ccaacttgtt tattgcagct tataatggtt acaaataaag
2461 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
2521 gtccaaactc atcaatgtat cttatcatgt ctggatcggg aattaattcg gcgcagcacc
2581 atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa
2641 ccatctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
2701 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
2761 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
2821 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
2881 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
2941 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctgt taacagcttg
3001 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
3061 cgccttgcag cacatccccc tttcgccagt tggcgtaata gcgaagaggc ccgcaccgat
3121 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
3181 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
3241 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
3301 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
3361 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
3421 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
3481 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
3541 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
3601 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
3661 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
3721 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
3781 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
3841 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
3901 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
3961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
4021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
4081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
4141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
4201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
4261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
4321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
4381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
4441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
4501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
4561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
4621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
4681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
4741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
4801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
4861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
4921 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
4981 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
5041 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
5101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
5161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
5221 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
5281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
5341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
5401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
5461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
5521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
5581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
5641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
5701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
5761 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
5821 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
5881 ca
//
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