| 质粒名称: | pGD-γb |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 196327 |
| 存储实验室: | Zhenghe Li |
|
载体骨架基本信息 |
|
| 载体名称: | pGD |
| 载体出品公司: | Clontech |
| 空载体大小: | 7707 bp |
| 完整质粒大小: | 7707 bp |
| 载体类型: | Plant Expression |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: |
barley stripe mosaic virus γb
Alt nameBSMV γb
|
| 物种来源: | barley stripe mosaic virus |
| 启动子: | duplicated cauliflower mosaic virus 35S promoter |
| 克隆方法: | Ligation Independent Cloning |
| 5’测序引物 | 35S promoter/forward primer: CTATCCTTCGCAAGACCCTTC |
| 3’测序引物 | Nos terminator/reverse primer: TCATCGCAAGACCGGCAA |
|
文献引用方法 |
|
| 材料和方法部分: | pGD-γb was a gift from Zhenghe Li (Addgene plasmid # 196327 ; http://n2t.net/addgene:196327 ; RRID:Addgene_196327) |
| 参考文献部分: | Construction of a Sonchus Yellow Net Virus minireplicon: a step toward reverse genetic analysis of plant negative-strand RNA viruses. Ganesan U, Bragg JN, Deng M, Marr S, Lee MY, Qian S, Shi M, Kappel J, Peters C, Lee Y, Goodin MM, Dietzgen RG, Li Z, Jackson AO. J Virol. 2013 Oct;87(19):10598-611. doi: 10.1128/JVI.01397-13. Epub 2013 Jul 24. 10.1128/JVI.01397-13 PubMed 23885070 |
pGD-γb
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGD-γb载体质粒基本信息
pGD-γb质粒图谱载体图谱和pGD-γb载体序列质粒序列多克隆位点信息
pGD-γb质粒载体简介
pGD-γb质粒序列载体序列
LOCUS pGD-γb 7707 bp ds-DNA circular SYN 12-APR-2023
DEFINITION 35S promoter-driven expression of the barley stripe mosaic virus
gamma-b RNA silencing suppressor.
ACCESSION .
VERSION .
KEYWORDS pGD-gamma-b
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7707)
AUTHORS Ganesan U, Bragg JN, Deng M, Marr S, Lee MY, Qian S, Shi M, Kappel
J, Peters C, Lee Y, Goodin MM, Dietzgen RG, Li Z, Jackson AO
TITLE Construction of a Sonchus Yellow Net Virus minireplicon: a step
toward reverse genetic analysis of plant negative-strand RNA
viruses.
JOURNAL J Virol. 2013 Oct;87(19):10598-611. doi: 10.1128/JVI.01397-13. Epub
2013 Jul 24.
PUBMED 23885070
REFERENCE 2 (bases 1 to 7707)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 12, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7707
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 5..29
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
promoter 179..524
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 467..487
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
terminator 1158..1410
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 1432..1456
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 2757..3386
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 3815..4888
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 4954..5148
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(5384..5406)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 5492..5632
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 5547..5566
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(5647..5664)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5818..6406)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5898..5917)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6493..7287)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
1 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
61 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat acgcgtcatg gtggagcacg
121 acactcttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
181 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
241 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
301 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
361 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
421 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
481 acccttcctc tatataagga agttcatttc atttggagag gacacgctga aatcaccagt
541 ctctctctac aagatctcga gatgatggct actttctctt gtgtgtgttg tggtacctta
601 actacaagta cttactgtgg taagagatgt gagcgaaagc atgtatattc tgaaacaaga
661 aataagagat tggaacttta caagaagtat ctattggaac cgcaaaaatg cgccctgaat
721 ggaatcgttg gacacagttg tggaatgcca tgctccattg cggaagaggc ttgtgatcaa
781 ctgccaatcg tgagtaggtt ctgtggccaa aagcatgcgg atctgtatga ttcacttctg
841 aaacgttctg aacaggagtt acttcttgaa tttctccaga agaagatgca ggagctgaaa
901 ctttctcata tcgtaaaaat ggctaagctt gaaagtgagg ttaacgcaat acgtaagtcc
961 gtagcttctt cttttgaaga ttctgttgga tgtgatgatt cttcttccgt ttctaagtaa
1021 aaaaaaaaaa aaaatgtttg atcagatcat tcaaatctga tggtgcccat caaccatatg
1081 atgggagtgt ttgcactgca gtcgacggta ccgcgggccc gggatccacc ggatctagat
1141 aactgatcgt cgacagagct cgttcaaaca tttggcaata aagtttctta agattgaatc
1201 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
1261 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
1321 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
1381 cgcgcgcggt gtcatctatg ttactagatc gggaattaaa ctatcagtgt ttgacaggat
1441 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
1501 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
1561 tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
1621 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
1681 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
1741 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
1801 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
1861 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
1921 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
1981 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
2041 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
2101 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
2161 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac
2221 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
2281 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
2341 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
2401 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa
2461 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg
2521 atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa
2581 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
2641 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
2701 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
2761 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
2821 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
2881 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
2941 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
3001 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
3061 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
3121 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
3181 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
3241 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
3301 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
3361 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
3421 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
3481 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
3541 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
3601 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
3661 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
3721 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca
3781 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc
3841 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
3901 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
3961 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
4021 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
4081 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
4141 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
4201 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
4261 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
4321 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
4381 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
4441 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
4501 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
4561 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
4621 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
4681 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
4741 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
4801 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
4861 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
4921 agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc
4981 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
5041 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
5101 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
5161 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
5221 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
5281 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca
5341 tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
5401 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
5461 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
5521 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
5581 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
5641 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
5701 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
5761 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
5821 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
5881 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
5941 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
6001 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
6061 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
6121 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
6181 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
6241 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
6301 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
6361 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
6421 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
6481 gcattctagg tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc
6541 aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc
6601 cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc
6661 aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc
6721 gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc
6781 gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc
6841 gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca
6901 atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt
6961 ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat
7021 gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt
7081 tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata
7141 ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt
7201 agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa
7261 ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct acagtattta
7321 aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc
7381 taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac
7441 atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat
7501 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
7561 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
7621 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
7681 gctgccggtc ggggagctgt tggctgg
//
