| 质粒名称: | p426GPD |
|---|---|
| 出品公司: | Addgene |
| 存储实验室: | Vladislav Verkhusha |
|
载体骨架基本信息 |
|
| 载体名称: | p426 GPD (plasmid)、pRS426 (ATCC 77107) |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 6637 bp |
| 载体类型: | plasmid |
| 筛选标记: | ampR; URA3 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 启动子: | GPD,<-,2339-3019 |
| 克隆方法: | 限制性内切酶 |
| 克隆位点: | Xhol; Sall; Clal; HindIlI; EcoRI; Smal; BamHI; Spel |
买家导航
p426GPD载体质粒基本信息
p426GPD质粒图谱载体图谱和p426GPD载体序列质粒序列多克隆位点信息
p426GPD质粒载体简介
p426GPD质粒序列载体序列
LOCUS p426GPD 6606 bp DNA circular SYN 26-NOV-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6606)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 26, 2024 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6606
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 69..1411
/label=2u ori
/note="yeast 2u plasmid origin of replication"
protein_bind 401..448
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
promoter 1438..1542
/gene="bla"
/label=AmpR promoter
CDS 1543..2403
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2574..3162
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 3486..3516
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3524..3540
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3548..3564
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 3585..3603
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 3628..4271
/gene="S. cerevisiae TDH3"
/label=GAP promoter
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase; also known as the TDH3 promoter"
primer_bind 4277..4293
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4327..4343)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
terminator 4346..4593
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
promoter complement(4612..4630)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4640..4656)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 4801..5256
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(5387..6190)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(6191..6406)
/gene="S. cerevisiae URA3"
/label=URA3 promoter
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc
3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa
3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
3601 agggaacaaa agctggagct cagtttatca ttatcaatac tcgccatttc aaagaatacg
3661 taaataatta atagtagtga ttttcctaac tttatttagt caaaaaatta gccttttaat
3721 tctgctgtaa cccgtacatg cccaaaatag ggggcgggtt acacagaata tataacatcg
3781 taggtgtctg ggtgaacagt ttattcctgg catccactaa atataatgga gcccgctttt
3841 taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac
3901 catcagttca taggtccatt ctcttagcgc aactacagag aacaggggca caaacaggca
3961 aaaaacgggc acaacctcaa tggagtgatg caacctgcct ggagtaaatg atgacacaag
4021 gcaattgacc cacgcatgta tctatctcat tttcttacac cttctattac cttctgctct
4081 ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct
4141 acttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc
4201 ttaaacttct taaattctac ttttatagtt agtctttttt ttagttttaa aacaccagaa
4261 cttagtttcg acggattcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa
4321 gcttatcgat accgtcgacc tcgagtcatg taattagtta tgtcacgctt acattcacgc
4381 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
4441 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
4501 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
4561 aaggttttgg gacgctcgaa ggctttaatt tgcggccggt acccaattcg ccctatagtg
4621 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
4681 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
4741 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcg
4801 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
4861 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
4921 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
4981 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
5041 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
5101 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
5161 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
5221 acgcgaattt taacaaaata ttaacgttta caatttcctg atgcggtatt ttctccttac
5281 gcatctgtgc ggtatttcac accgcatagg gtaataactg atataattaa attgaagctc
5341 taatttgtga gtttagtata catgcattta cttataatac agttttttag ttttgctggc
5401 cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag
5461 catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga
5521 ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca
5581 caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag
5641 caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat
5701 attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag
5761 gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca
5821 caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact
5881 gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt
5941 acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga
6001 tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta
6061 attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga
6121 tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag
6181 ctttcgacat gatttatctt cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga
6241 atactgggca atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc
6301 tgtgctcctt ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaaa
6361 gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga attgaattga aaagctgtgg
6421 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
6481 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
6541 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
6601 gcgcga
//
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