| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141372 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | 9786 bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | Stellar E.coli (HST08 strain) |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-nsp6 |
| 物种来源: | SARS-CoV-2 |
| 基因突变: | human codon optimized |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (C terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: |
pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141372)
|
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro 9786 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9786)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 9786)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9786
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2338..3519
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2568..3510
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3467..3487
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 4441..4464
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 4501..4524
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 4536..5109
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4703..4720)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4930..4949
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 5142..5741
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5142..5161)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5638..5658
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 5755..6343
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5808..5828)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6226..6237)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6550..7183
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7311..7327)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7311..7327)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7324..7346)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7335..7351
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7359..7389)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7404..7425
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7542..7559)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7713..8301)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7793..7812)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8472..9332)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9095..9114
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9333..9437)
/gene="bla"
/label=AmpR promoter
polyA_signal 9485..9619
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9522..9541)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9576..9595
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
3541 gccgccacca tgagcgcggt taaacggacc ataaagggaa ctcaccattg gttgttgctt
3601 accatcctca cgtcccttct cgtgctggtc caaagtacac aatggagttt gtttttcttc
3661 ttgtacgaga atgcattcct ccctttcgcc atggggatta tagcgatgtc cgcctttgcc
3721 atgatgtttg tgaagcacaa acacgctttt ctgtgcctct tcctcctgcc gtcactggcc
3781 acagtggcgt acttcaacat ggtctacatg ccagcaagtt gggtaatgcg gataatgact
3841 tggcttgaca tggtggatac atccttgagt ggatttaaac ttaaagactg tgttatgtat
3901 gcatccgctg ttgtactcct gatactcatg actgccagga ctgtatatga cgacggcgct
3961 agaagagtat ggacactgat gaacgtattg acacttgtgt ataaggtgta ttacggtaat
4021 gcgctcgacc aggctatttc catgtgggcc ctgattattt ctgttacatc taactactca
4081 ggagtagtga ctaccgttat gtttttggcg agagggattg tctttatgtg cgttgaatat
4141 tgtccaatct ttttcataac cgggaatact ttgcagtgca tcatgcttgt ctactgcttt
4201 ctcgggtatt tctgcacttg ttactttggc ctgttttgcc tgcttaatcg ctacttcaga
4261 ctcacacttg gtgtctatga ctacctggta tccactcagg agtttagata catgaatagt
4321 cagggtttgt tgcctccaaa aaacagcatt gatgccttca agctcaatat aaaacttctc
4381 ggagtcggcg gtaaaccttg tattaaagta gcgactgttc agctcgaagg cggcggggga
4441 tggagccatc cacaattcga gaaaggcggt ggttcaggag gaggtagcgg gggtggatca
4501 tggtcacatc cgcagtttga aaagtaagga tcccgcccct ctccctcccc cccccctaac
4561 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
4621 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
4681 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
4741 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
4801 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
4861 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
4921 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
4981 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
5041 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
5101 cgatgataag cttgccacaa cccacaagga gacgaccttc catgaccgag tacaagccca
5161 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
5221 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
5281 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
5341 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
5401 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
5461 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
5521 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
5581 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
5641 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
5701 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta gacgcgtctg gaacaatcaa
5761 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
5821 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
5881 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
5941 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
6001 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
6061 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
6121 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
6181 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
6241 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
6301 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca
6361 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
6421 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
6481 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
6541 agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
6601 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
6661 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
6721 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
6781 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
6841 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
6901 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
6961 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
7021 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
7081 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
7141 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
7201 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga
7261 cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
7321 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
7381 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
7441 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
7501 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
7561 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
7621 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
7681 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
7741 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
7801 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
7861 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
7921 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
7981 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
8041 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
8101 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
8161 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
8221 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
8281 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
8341 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
8401 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
8461 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
8521 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
8581 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
8641 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
8701 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
8761 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
8821 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
8881 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
8941 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
9001 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
9061 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
9121 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
9181 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
9241 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
9301 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
9361 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
9421 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
9481 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
9541 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
9601 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
9661 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
9721 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
9781 agtagt
//
