| 质粒名称: | mCherry-Actin-C-18 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 54967 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mCherry-C1 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4750bp |
| 完整质粒大小: | 5769bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Actin、NM_001101.3 |
| 物种来源: | H. sapiens (human) |
| 启动子: | CMV |
| 标签/融合蛋白: | mCherry (N terminal on backbone) |
| 克隆方法: | 未知 |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | mCherry-Actin-C-18 was a gift from Michael Davidson (Addgene plasmid # 54967 ; http://n2t.net/addgene:54967 ; RRID:Addgene_54967) |
| 参考文献部分: | Fluorescent protein tracking and detection: fluorescent protein structure and color variants. Rizzo MA, Davidson MW, Piston DW. Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 10.1101/pdb.top63. 10.1101/pdb.top63 PubMed 20150100 |
买家导航
mCherry-Actin-C-18载体质粒基本信息
mCherry-Actin-C-18质粒图谱载体图谱和mCherry-Actin-C-18载体序列质粒序列多克隆位点信息
mCherry-Actin-C-18质粒载体简介
mCherry-Actin-C-18质粒序列载体序列
LOCUS mCherry-Actin-C-18 5769 bp DNA circular SYN 25-AUG-2024
DEFINITION Localization: Actin, Excitation: 587, Emission: 610.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5769)
AUTHORS Rizzo MA, Davidson MW, Piston DW
TITLE Fluorescent protein tracking and detection: fluorescent protein
structure and color variants.
JOURNAL Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi:
10.1101/pdb.top63.
PUBMED 20150100
REFERENCE 2 (bases 1 to 5769)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi";
pages: " 10.1101/pdb.top63"
FEATURES Location/Qualifiers
source 1..5769
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 40..343
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 344..547
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 498..518
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 586..595
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 592..1299
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(737..755)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1005..1024
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 2608..2729
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2645..2664)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2699..2718
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2736..3191)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2873..2894)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3085..3104
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3218..3322
/gene="bla"
/label=AmpR promoter
primer_bind complement(3347..3367)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3391..3601
/label=SV40 promoter
/note="SV40 early promoter"
promoter 3413..3609
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 3460..3595
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3644..4438
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 3644..4438
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3698..3717)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4308..4327
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4626..4645)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4670..4717
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5046..5634
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5535..5554
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
61 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
121 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
181 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
241 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
301 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
361 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
421 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
481 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
541 cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac catggtgagc
601 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
661 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
721 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
781 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
841 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
901 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
961 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
1021 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
1081 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
1141 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
1201 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
1261 ggccgccact ccaccggcgg catggacgag ctgtacaagt ccggactcag atctggcagc
1321 ggtggaggca gcgcatccgg cggaagcgga agcgatgatg atatcgccgc gctcgtcgtc
1381 gacaacggct ccggcatgtg caaggccggc ttcgcgggcg acgatgcccc ccgggccgtc
1441 ttcccctcca tcgtggggcg ccccaggcac cagggcgtga tggtgggcat gggtcagaag
1501 gattcctatg tgggcgacga ggcccagagc aagagaggca tcctcaccct gaagtacccc
1561 atcgagcacg gcatcgtcac caactgggac gacatggaga aaatctggca ccacaccttc
1621 tacaatgagc tgcgtgtggc tcccgaggag caccccgtgc tgctgaccga ggcccccctg
1681 aaccccaagg ccaaccgcga gaagatgacc cagatcatgt ttgagacctt caacacccca
1741 gccatgtacg ttgctatcca ggctgtgcta tccctgtacg cctctggccg taccactggc
1801 atcgtgatgg actccggtga cggggtcacc cacactgtgc ccatctacga ggggtatgcc
1861 ctcccccatg ccatcctgcg tctggacctg gctggccggg acctgactga ctacctcatg
1921 aagatcctca ccgagcgcgg ctacagcttc accaccacgg ccgagcggga aatcgtgcgt
1981 gacattaagg agaagctgtg ctacgtcgcc ctggacttcg agcaagagat ggccacggct
2041 gcttccagct cctccctgga gaagagctac gagctgcctg acggccaggt catcaccatt
2101 ggcaatgagc ggttccgctg ccctgaggca ctcttccagc cttccttcct gggcatggag
2161 tcctgtggca tccacgaaac taccttcaac tccatcatga agtgtgacgt ggacatccgc
2221 aaagacctgt acgccaacac agtgctgtct ggcggcacca ccatgtaccc tggcattgcc
2281 gacaggatgc agaaggagat cactgccctg gcacccagca caatgaagat caagatcatt
2341 gctcctcctg agcgcaagta ctccgtgtgg atcggcggct ccatcctggc ctcgctgtcc
2401 accttccagc agatgtggat cagcaagcag gagtatgacg agtccggccc ctccatcgtc
2461 caccgcaaat gcttctaggg atccaccgga tctagataac tgatcataat cagccatacc
2521 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
2581 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
2641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
2701 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt
2761 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
2821 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
2881 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
2941 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
3001 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
3061 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
3121 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
3181 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
3241 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
3301 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
3361 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
3421 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
3481 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
3541 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
3601 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
3661 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
3721 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
3781 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
3841 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
3901 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
3961 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
4021 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
4081 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
4141 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
4201 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
4261 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
4321 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
4381 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
4441 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
4501 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
4561 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
4621 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
4681 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
4741 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
4801 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
4861 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
4921 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
4981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
5041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
5101 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
5161 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
5221 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
5281 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
5341 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
5401 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
5461 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
5521 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
5581 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
5641 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
5701 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata
5761 gtaatcaat
//
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