pEGFP(C3)-Nup153

LOCUS       pEGFP(C3)-Nup153                9131 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  GFP-tagged mammalian expression of Nup153.
ACCESSION   .
VERSION     .
KEYWORDS    pEGFP(C3)-Nup153
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Duheron V, Chatel G, Sauder U, Oliveri V, Fahrenkrog B
  TITLE     Structural characterization of altered nucleoporin Nup153 expression
            in human cells by thin-section electron microscopy.
  JOURNAL   Nucleus. 2014;5(6):601-12. doi: 10.4161/19491034.2014.990853.
  PUBMED    25485891
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9131
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             613..1329
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             613..1329
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5919..6040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5956..5975)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6010..6029
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6047..6502)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6184..6205)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6396..6415
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6529..6633
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6635..6992
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(6658..6678)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6843..6978
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6905..6924
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7027..7821
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7081..7100)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7691..7710
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8009..8028)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8053..8100
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8429..9017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8918..8937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt actcagatct cgagctcaag cttatggcct cgggagccgg aggagtcgga
     1381 gggggcggtg gcggcaagat ccggacgcgg cgttgccacc aggggccaat taagccttac
     1441 cagcaggggc gacaacagca tcagggcatt cttagcaggg ttacagaatc tgttaagaat
     1501 attgtgccag ggtggctaca aagatacttc aacaagaatg aagatgtatg cagctgttca
     1561 acagacacaa gcgaggttcc acgctggcca gaaaataaag aggaccatct ggtatatgcc
     1621 gatgaggaga gctctaatat tactgatggg agaatcacac ctgagccagc agtcagtaat
     1681 acagaagaac cttcaacaac tagtactgct tcaaattatc cagatgtgtt aacaaggcct
     1741 tctcttcatc ggagccatct gaatttttcc atgttggaat cccctgcatt acactgtcag
     1801 ccatctacat cctcggcatt cccaattggc agttcgggat tttcccttgt aaaggaaatt
     1861 aaagattcta cctctcagca tgatgatgat aacatctcaa ctaccagtgg tttttcttca
     1921 agagcttctg ataaagatat aactgtttca aagaacactt cattgccacc tctgtggtcc
     1981 ccagaagctg aacgttctca ctcactctca cagcacactg ccaccagctc aaaaaaacca
     2041 gcattcaact tgtctgcctt tggaacactt tccccttcac ttgggaattc ttcaatcctt
     2101 aaaaccagtc agcttggaga ttctcctttt tatcctggaa aaacaacgta cggtggggca
     2161 gcagctgctg taagacagtc taaactacga aatacacctt atcaggcacc agttagaaga
     2221 caaatgaaag ctaagcaact cagtgcacaa tcttacggtg tgaccagttc aacagctcgg
     2281 cgaatattgc agtctttaga gaagatgtca agccctttag cggatgcaaa aagaattcca
     2341 tccattgttt cttctcctct gaattctcct cttgatagga gtgggataga tatcacagat
     2401 tttcaggcca aaagagaaaa ggtggattct caatatcctc ctgttcagag acttatgacc
     2461 ccaaagccag tttccatagc aacaaatcga agtgtttatt ttaaaccatc tctgactcct
     2521 tctggtgaat tcaggaagac taatcaaaga atagataaca agtgcagtac tggatatgaa
     2581 aaaaatatga cacccggaca aaatagagaa caacgagaaa gtggcttttc atatccaaat
     2641 ttcagtttgc ctgcagccaa tggtttatct tctggagtag gtggtggagg tggcaagatg
     2701 agacgagaaa gaacacgctt tgttgcttct aaacctctgg aggaggagga aatggaagtt
     2761 ccagtattac cgaaaatctc tctaccgatc accagttctt cactgcctac ctttaatttt
     2821 agttcccctg agatcacaac ttcctctcca tcacccatca attcgtctca agcattaaca
     2881 aacaaggtac aaatgacctc tccgagcagc actggcagtc ccatgtttaa attttcatct
     2941 ccaatcgtaa aatctactga ggcaaatgta ctacctccat catctattgg atttacattt
     3001 agtgtgcctg ttgcaaaaac agcagaactt tctggttcta gtagtacttt agaaccaatt
     3061 ataagtagtt cagctcatca tgtcactaca gtgaacagta caaattgtaa gaagacacca
     3121 cctgaagatt gtgagggtcc ttttagacct gcagaaatcc tgaaagaagg aagtgttcta
     3181 gatattctga aaagccctgg tttcgcatcg ccgaagatag attctgttgc tgctcagccc
     3241 accgcaacaa gcccagtagt ttatacaaga ccagcaataa gtagcttttc ttctagtgga
     3301 attgggtttg gggagagttt aaaagctggg tcatcatggc agtgtgatac atgtctactc
     3361 cagaacaaag ttacagacaa caaatgcata gcctgtcaag cagcaaaatt gtcacccaga
     3421 gatactgcta aacagactgg aattgaaaca ccaaataaaa gtggcaaaac aactctttct
     3481 gcatcaggga caggctttgg agacaaattt aaaccagtga taggcacttg ggattgtgat
     3541 acctgtttag tgcaaaataa acctgaagca ataaaatgtg tagcctgtga aacaccgaaa
     3601 cctggaactt gtgtgaagcg agcccttaca ttgacagtgg tttcggaaag tgctgagact
     3661 atgactgctt catcttccag ctgcactgta accactggta ccttaggatt tggagataaa
     3721 ttcaaaaggc ccattggatc ttgggagtgt tcagtatgct gtgtttctaa taatgcagaa
     3781 gacaataagt gtgtgtcctg tatgtctgag aaaccaggaa gttcagtacc tacttcaagt
     3841 agcagcactg tacctgtctc tctgccttct ggaggctctc taggattgga aaagttcaag
     3901 aaacccgagg gaagctggga ctgtgaattg tgcctagtgc agaataaggc agactctacc
     3961 aaatgtttgg catgtgaaag tgcaaagcca ggcacaaaat ctgggtttaa aggctttgac
     4021 acatcttcct catcttcgaa ctcagcagcc tcctcatcct tcaaatttgg tgtctcatca
     4081 tcctcttctg ggccttctca gactttaaca agcactggaa attttaaatt tggagatcag
     4141 ggaggattca aaataggtgt gtcatctgat tctgggtcta taaaccccat gagtgaaggc
     4201 tttaaatttt ctaaaccaat aggagatttt aaatttggag tttcatctga atctaagccc
     4261 gaagaagtta aaaaagatag taagaatgat aattttaagt ttggactttc ttctggttta
     4321 agcaacccag tttctttaac tccatttcaa tttggggtat ctaatcttgg acaggaagaa
     4381 aagaaagagg aactgcccaa atcttcctct gcaggtttta gctttggtac aggtgttatt
     4441 aactccaccc ctgctcctgc taacaccata gtgacctctg agaacaagag cagcttcaac
     4501 cttggaacca tagaaaccaa gagtgcttca gtggctcctt tcacatgtaa gacatcagaa
     4561 gctaaaaaag aagaaatgcc tgccaccaaa ggaggattct cttttggcaa cgtggagcct
     4621 gcctctctgc catctgcctc agtgtttgtt ttgggaagga cagaagagaa acagcaagag
     4681 cctgtcactt ctacttccct agtttttggg aagaaagctg acaatgaaga gccaaagtgt
     4741 caaccagtgt tttcctttgg gaattcagag caaaccaaag atgagaattc ttcaaagtcc
     4801 acatttagtt ttagtatgac aaaaccatct gagaaggaat ctgaacagcc agcaaaagcc
     4861 acttttgcct ttggagctca aactagtact acagctgatc aaggtgcagc aaagccagtt
     4921 tttagtttct tgaacaacag ttcctctagt tcaagtacac cagccacttc tgctggtggt
     4981 ggcatatttg gtagttccac ctcttcctcc aatccacctg tggctacctt tgtgtttgga
     5041 cagtccagca atcctgtgag cagctctgcc tttggtaaca ctgctgaatc cagcacctct
     5101 cagtctttgc tattttctca agatagcaaa ctagcaacca catccagcac aggtacagct
     5161 gtcaccccat ttgtctttgg tccaggagcc agcagtaata atactaccac ctctggtttc
     5221 ggctttggag ccacaaccac atctagctct gcaggatcct cctttgtatt tggaactgga
     5281 ccctcagcac catctgccag tccagcattt ggtgctaacc agaccccaac atttggacaa
     5341 agtcaaggtg ccagccagcc caatccccca ggctttggat ctatatcatc ttccacagca
     5401 ttatttccca ctggttctca gcctgcacca cctacttttg ggacagtgtc aagcagtagc
     5461 cagccccctg tgtttggaca gcaacctagt cagtctgcat ttggctctgg aacaactcct
     5521 aattctagtt cggctttcca gtttggcagc agcactacaa atttcaactt cacaaacaac
     5581 agtccatcag gagtgttcac atttggtgca aattctagca cacctgcagc ctcagcccag
     5641 ccttcaggct cggggggctt tccatttaac cagtctccag cagcatttac agtggggtca
     5701 aatgggaaaa atgtgttctc ttcttctgga acttcattct ctggtcgcaa gataaagact
     5761 gctgttagac gcaggaaatt atccccccgg gatccaccgg atctagataa ctgatcataa
     5821 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5881 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5941 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6001 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt
     6061 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     6121 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     6181 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     6241 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     6301 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     6361 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     6421 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     6481 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6541 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6601 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
     6661 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6721 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     6781 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6841 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6901 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6961 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
     7021 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     7081 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     7141 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     7201 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     7261 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     7321 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     7381 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     7441 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     7501 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     7561 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     7621 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     7681 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     7741 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     7801 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     7861 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     7921 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     7981 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     8041 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
     8101 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     8161 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     8221 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
     8281 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8341 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8401 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8461 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8521 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8581 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8641 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8701 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8761 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8821 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8881 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8941 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9001 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9061 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9121 ccgccatgca t
//