质粒名称: | pExchange_tdk_BT1311p-tetR-1 |
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出品公司: | Addgene |
目录编号: | 90325 |
存储实验室: | Vladislav Verkhusha |
载体骨架基本信息 |
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载体名称: | pExchange-tdk |
载体出品公司: | Clontech |
空载体大小: | 4038 bp |
完整质粒大小: | 4989 bp |
载体类型: | 细菌表达 |
筛选标记: | Erythromycin |
载体抗性: | 氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | Pir1 |
拷贝数: | 未知 |
插入基因信息 |
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插入基因名称: | TetR |
克隆方法: | Gibson Cloning |
5’测序引物 | GGAGAGACAGCCGAATACGA |
3’测序引物 |
AACACTTAACGGCTGACATGG
(Common Sequencing Primers)
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文献引用方法 |
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材料和方法部分: | pExchange_tdk_BT1311p-tetR-1 was a gift from Andrew Goodman (Addgene plasmid # 90325 ; http://n2t.net/addgene:90325 ; RRID:Addgene_90325) |
参考文献部分: | Engineered Regulatory Systems Modulate Gene Expression of Human Commensals in the Gut. Lim B, Zimmermann M, Barry NA, Goodman AL. Cell. 2017 Apr 20;169(3):547-558.e15. doi: 10.1016/j.cell.2017.03.045. 10.1016/j.cell.2017.03.045 PubMed 28431252 |
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pExchange_tdk_BT1311p-tetR-1载体质粒基本信息
pExchange_tdk_BT1311p-tetR-1质粒图谱载体图谱和pExchange_tdk_BT1311p-tetR-1载体序列质粒序列多克隆位点信息
pExchange_tdk_BT1311p-tetR-1质粒载体简介
pExchange_tdk_BT1311p-tetR-1质粒序列载体序列
LOCUS pExchange_tdk_BT1311p-tetR-1 7210 bp DNA circular SYN 25-AUG-2024 DEFINITION Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702 . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7210) AUTHORS Lim B, Zimmermann M, Barry NA, Goodman AL TITLE Engineered Regulatory Systems Modulate Gene Expression of Human Commensals in the Gut. JOURNAL Cell. 2017 Apr 20;169(3):547-558.e15. doi: 10.1016/j.cell.2017.03.045. PUBMED 28431252 REFERENCE 2 (bases 1 to 7210) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3 https://www.biofeng.com COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1016/j.cell.2017.03"; journalName: "Cell"; date: "2017-04-20- 20"; volume: "169"; issue: "3"; pages: "547-558.e15" FEATURES Location/Qualifiers source 1..7210 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(30..890) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 653..672 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" CDS 4713..5336 /codon_start=1 /gene="tetR from transposon Tn10" /product="tetracycline repressor TetR" /label=TetR /note="TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline." /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS" primer_bind complement(4786..4805) /label=Tet-R /note="Tetracycline resistance gene, reverse primer" rep_origin 6441..6829 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" oriT 7000..7109 /note="incP origin of transfer" ORIGIN 1 acgcgttatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 61 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 901 tttcaatcat ggccgcggga ttaaaagtcg gggattggtg aacaaaaagg tgtttctctc 961 tttaagagaa atatcgtttt gctaaacagt tgatattgag gtatcatttt atcgtaaaag 1021 acatttttgc tcaacaattg cttgacggaa atcaacaaat tttagcattt tgtaaaaaag 1081 tcgctatata atttggtgaa ttggagttat tttcatattt ttgcatcccg aagagtttct 1141 cttaaagaga gaaacatctt ttgcatacct tttccgaccg aatttttatg tcgtaaagag 1201 gggctttgca gggggtggac tcagaaagat gagaatagat gactattgta gttgaaacac 1261 atagaaagtt gctgatatac agaccgatac gcatatcggg atgaaccatg agtacgttct 1321 tttctcaaaa aacataaata ttcgaaaaga gatgcaataa attaaggaga ggttataatg 1381 aacaaagtaa atataaaaga tagtcaaaat tttattactt caaaatatca catagaaaaa 1441 ataatgaatt gcataagttt agatgaaaaa gataacatct ttgaaatagg tgcagggaaa 1501 ggtcatttta ctgctggatt ggtaaagaga tgtaattttg taacggcgat agaaattgat 1561 tctaaattat gtgaggtaac tcgtaataag ctcttaaatt atcctaacta tcaaatagta 1621 aatgatgata tactgaaatt tacatttcct agccacaatc catataaaat atttggcagc 1681 ataccttaca acataagcac aaatataatt cgaaaaattg tttttgaaag ttcagccaca 1741 ataagttatt taatagtgga atatggtttt gctaaaatgt tattagatac aaacagatca 1801 ctagcattgc tgttaatggc agaggtagat atttctatat tagcaaaaat tcctaggtat 1861 tatttccatc caaaacctaa agtggatagc acattaattg tattaaaaag aaagccagca 1921 aaaatggcat ttaaagagag aaaaaaatat gaaacttttg taatgaaatg ggttaacaaa 1981 gagtacgaaa aactgtttac aaaaaatcaa tttaataaag ctttaaaaca tgcgagaata 2041 tatgatataa acaatattag tttcgaacaa tttgtatcgc tatttaatag ttataaaata 2101 tttaacggct aaaaacaata ggccacatgc aactgtaaat gtttacgcgt cctcggtacc 2161 gcttcttcca caacagtctg cggttcctgt actatcacag gttcatcttc tcccggtgct 2221 gtcgcttcat tctcttccac gaccttctct tctatcaccg gacattcaac ttcctctatt 2281 acaggggctt gttcttcaac aatgggggct tcactttccg cttccgctac aggagaaggc 2341 gaggcttcca ccggcacagc acttatctct tccgacaact gttccaaacg ctcctgcata 2401 cgtaggatgc tccgcttcaa cagttcagac aaagtctgag tcggctcttt agaaaacgta 2461 ttcatgagta gcttcagctc atgaacatcc agctcaatat ctgttaatag cttctgtttc 2521 atttccatcc attgttatta tggtgcgaat gtaggaataa tttatgcaat aacttaaaat 2581 aatggataat ttaacagttc ttgtacatcg aattgtttac ctttgtccac tcatttattt 2641 aatcaagaaa gcacaaaaat cacatggtat tattttcaga agaccacata caagagacca 2701 gaagaagagg tagaatagag gtaatctgcg gttcaatgtt ctccggaaag acggaggaat 2761 tgattcgtag gatgaagaga gccaagttcg cacgccagcg tgtagagata ttcaaaccgg 2821 ccatcgacac ccgttattcg gaagaagacg tggtatcaca cgacagtcat tccatcgcgt 2881 ccacccctat cgattcatcg gctagtatcc tgttgttcac ttctgaaata gatgtagtcg 2941 gcatcgacga agcacagttc tttgatgacg gactgattga cgtatgcagg cagcttgcca 3001 ataacggcat ccgtgtcatc atcgccggac tggatatgga ctttaaaggg aatcccttcg 3061 gaccgatgcc tcaattatgt gccattgccg atgaagtttc caaagttcat gcgatctgcg 3121 tgaagtgcgg tgatctggca tctttctctc atcgtaccgt caagaatgac aagcaggtac 3181 ttctaggaga gacagccgaa tacgagccgc tttgcaggga gtgctacctc cgtgcacggg 3241 gagaggacgg acagaagata taaactctat ttaaaacata tatggaaaga aagaagataa 3301 cattcgagtc gacggtatcg ataagcttga tatcgaattc ctgcaatgcg aatgtagagg 3361 ggaaaataga tgggaataat attagtttta ttattccaga tggattggag aacttgacgt 3421 ttgcgcctac tattgttatt tctgataaag caacaataac tcctgcttct ggagtagaac 3481 aagatttttc atctcctgta atatacacag tgacttccga agatggaatt gtaactacta 3541 aatatacagt ttctgtatca ggtcaaataa gagattttgg attcgatgaa tgggagacta 3601 tcaaatcatc tacatcgggt tctacagaac aatatgatgt tcctaaagga ctttatggta 3661 ctagtaatcc gggaattatg acaattaacg agatgtttgg ttcaaatctt ccagagctgt 3721 tctcatatac agtttctcct gtagaaggac aaagtggtaa agcagctaaa ttgcaaacag 3781 tttatactta catgttttta aatggtatgg attttaatgc attattagga gggttggtac 3841 cttatgtaac agcaggctca ttatttacag gtatctttaa aactgatatg aataatactt 3901 tgaatagtac aaagtttggt gttccttttg ttggggaacc tgctactttt actggctatt 3961 ataaatatac tccagggaca acatattata ataataaaaa tgaagtcgta gctgataaaa 4021 cagatgaatg ttcaatatat gcagttttgt atgaagaact tttggatgaa gagaataaca 4081 acattccgtt gaatggagac tacaatgatc ctgatctata tattggaagc tctaaacgta 4141 ttgtaatgaa ggccgttttg gaagatggaa cagcgaagag tgattggact aagttctcta 4201 tcccatttaa attattggaa gggaaaagtt atgatgcaac taaaaaatat tatttagcta 4261 ttgtgtgttc ctcaagtgct gaaggagact actttatggg cgctcctggt agtaccttat 4321 ttattgatag ttttacggta acacctcaat agtgtgttga gtataagaaa tatataacag 4381 aaaaagccgt taattccaat tgtatacatt aatgatctgg aagaagcaat gaaagctgct 4441 gttaagtctc cgaatcaggt attgttcctg acaggtgtat tcccatccgg taaacgcgga 4501 tactttgcag ttgatctgac tcaggaataa attataaatt aaggtaagaa gattgtagga 4561 taagctaatg aaatagaaaa aggatgccgt cacacaactt gtcggcattc ttttttgttt 4621 tattagttga aaatatagtg aaaaagttgc ctaaatatgt atgttaacaa attatttgtc 4681 gtaactttgc actccaaatc tgtttttaaa gaatgtctag attagataaa agtaaagtga 4741 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac 4801 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg 4861 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt 4921 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt 4981 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt 5041 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg 5101 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag 5161 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat 5221 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc 5281 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tcttaatgca 5341 attaaaatta acggcttttt tagttgtttt tatttaaata aattgtagtg ttttctctag 5401 aacgcatatc cgaatcctat attcaaataa ataggataca tagcgaatga gatggtatca 5461 aaatcctttt tgaagatatc gttcaagccc catgttaaat cagcatacac atttaaatgt 5521 ttgaatgctc tccactcacc gcctagttgc aatccccatt ggaatttacg cagattgtca 5581 gagaaatcat aagtagcaat gctttcacca gtaaaatcta ctcgcgaacc tgtctgatcc 5641 ggagtacgca agtgaccttc atatacatgt ccggagaagt ttctttcaat aagataagat 5701 acatacggac ctgctgagac tttccaacgt ttgcttactt tatagttggc aactactggg 5761 atagtcagat aagagttttt gactttagtc tttacaccac cggtccataa gccttgtaac 5821 tctccaccgt tggtgttgat aatcttcatc ccatagttct ttactgtagc ttctgtggtc 5881 atattcttgt tttcaagtcg tacccctaca gtcattcccc atttcgtgtc aaaccattta 5941 gttgcgtttc cttcaattga aatggcgagg ccaccagggg catagctatc tattttgcga 6001 atttcttcag gtagaggcaa tggagaagtt cctccaatac tgaaacctgc cttgattcca 6061 tattcccatc cgtgtaagta cgaccagatg ataccttgat ttctttcttc ttgcgcctgc 6121 gctgaaagtg tacttactaa tagtagtaca gctataatat tgagtaattt atatcttttc 6181 attttcttgt ttttttagtc ttcgcaaata agttctactt catcgacata cagtgtactg 6241 cctactgctc ccttaaaata agcaccgtct acactggacg aaagcactat acctaatttg 6301 tattttccat tctttaattt ggtatcatca attactcgtc cttttaccgg ttcgaaagga 6361 atttcaaaat cagtccattg atctgattca acaacatctt ccggtttaat tcgcgccacc 6421 gcggtggagg ggaattccca tgtcagccgt taagtgttcc tgtgtcactc aaaattgctt 6481 tgagaggctc taagggcttc tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta 6541 aaccttaaaa gctttaaaag ccttatatat tctttttttt cttataaaac ttaaaacctt 6601 agaggctatt taagttgctg atttatatta attttattgt tcaaacatga gagcttagta 6661 cgtgaaacat gagagcttag tacgttagcc atgagagctt agtacgttag ccatgagggt 6721 ttagttcgtt aaacatgaga gcttagtacg ttaaacatga gagcttagta cgtgaaacat 6781 gagagcttag tacgtactat caacaggttg aactgctgat cttcagatcc tctacgccgg 6841 acgcatcgtg gccggatcaa ttccgttttc cgctgcataa ccctgcttcg gggtcattat 6901 agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt 6961 tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg 7021 cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa 7081 cgggaatcct gctctgcgag gctggccggc taccgccggc gtaacagatg agggcaagcg 7141 gatggctgat gaaaccaagc caaccaggaa gggcagccca cctatcacgg aattgatccc 7201 cctcgaattg //
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