| 出品公司: | Origene |
|---|---|
| 载体名称: | pAAVS1-RFP-DNR |
| 质粒类型: | 哺乳动物细胞载体；腺病毒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 6.8kb |
| 5’测序引物: | CMV-F:CGCAAATGGGCGGTAGGCGTG (Invitrogen) |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | DH5 alpha; 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 产品目录号: |
GE100026
|
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 病毒 |
| 备注: |
买家导航
pAAVS1-RFP-DNR载体质粒基本信息
pAAVS1-RFP-DNR质粒图谱载体图谱和pAAVS1-RFP-DNR载体序列质粒序列多克隆位点信息
pAAVS1-RFP-DNR质粒载体简介
pAAVS1-RFP-DNR质粒序列载体序列
LOCUS pAAVS1-RFP-DNR 8165 bp DNA circular SYN 26-OCT-2024
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8165)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Oct 26, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
https://www.biofeng.com
COMMENT VNTDATE|1072224000|
COMMENT VNTDBDATE|1072224000|biofeng
COMMENT VNTNAME|pAAVS1-RFP-DNR|
COMMENT VNTAUTHORNAME|Ming|
FEATURES Location/Qualifiers
primer_bind complement(95..111)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 119..135
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(143..173)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 188..209
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(497..1085)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1256..2116)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
promoter complement(2117..2221)
/gene="bla"
/label=AmpR promoter
rep_origin complement(2503..2958)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 3171..3187
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 3278..3779
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
CDS complement(4381..4980)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
promoter complement(5020..5519)
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
enhancer 5637..5940
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 5941..6144
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 6170..6188
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 6247..6942
/codon_start=1
/product="red fluorescent protein from Entacmaea
quadricolor"
/label=TurboRFP
/note="mammalian codon-optimized"
CDS 6961..6990
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
CDS 7009..7032
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
primer_bind complement(7052..7068)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal 7079..7555
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
misc_feature 7630..8159
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
ORIGIN
1 atcactctcg ccggttggac tttagatcag aagggatctt gctgccgccc gaaagaggaa
61 gggctggaag aggaaggagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
121 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
181 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
241 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
301 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
361 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
421 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
481 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
541 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
601 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
661 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
721 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
781 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
841 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
901 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
961 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
1021 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
1081 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
1141 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
1201 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
1261 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
1321 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
1381 ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca ataaaccagc
1441 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
1501 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
1561 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
1621 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
1681 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
1741 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
1801 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
1861 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
1921 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
1981 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
2041 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
2101 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
2161 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
2221 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
2281 cctataaaaa taggcgtatc acgaggccct ttcgggtcgc gcgtttcggt gatgacggtg
2341 aaaacctctg acacatgcag ctcccgttga cggtcacagc ttgtctgtaa gcggatgccg
2401 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
2461 actatgcggc atcagagcag attgtactga gagtgcacca taaaattgta aacgttaata
2521 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caatagaccg
2581 aaatcggcaa aatcccttat aaatcaaaag aatagcccga gatagagttg agtgttgttc
2641 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
2701 ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt
2761 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
2821 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
2881 aggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
2941 cgccgctaca gggcgcgtac tatggttgct ttgacgtatg cggtgtgaaa taccgcacag
3001 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
3061 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
3121 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
3181 ggccagtgaa ttggaggcta cagtcagtgg agaggacttt cacaggctgt cgccgtgctc
3241 atttgataac tgcccgttat tcatgcgaca ctctagagag cacttccttc tcggcgctgc
3301 accacgtgat gtcctctgag cggatcctcc ccgtgtctgg gtcctctccg ggcatctctc
3361 ctccctcacc caaccccatg ccgtcttcac tcgctgggtt cccttttcct tctccttctg
3421 gggcctgtgc catctctcgt ttcttaggat ggccttctcc gacggatgtc tcccttgcgt
3481 cccgcctccc cttcttgtag gcctgcatca tcaccgtttt tctggacaac cccaaagtac
3541 cccgtctccc tggctttagc cacctctcca tcctcttgct ttctttgcct ggacaccccg
3601 ttctcctgtg gattcgggtc acctctcact cctttcattt gggcagctcc cctacccccc
3661 ttacctctct agtctgtgct agctcttcca gccccctgtc atggcatctt ccaggggtcc
3721 gagagctcag ctagtcttct tcctccaacc cgggccccta tgtccacttc aggacagcac
3781 tagtggacct aataacttct atagcataca ttatacgaag ttatattaag ggttccggat
3841 ctcgaccagc ttctgatgga attagaactt ggcaaaacaa tactgagaat gaagtgtatg
3901 tggaacagag gctgctgatc tcgttcttca ggctatgaaa ctgacacatt tggaaaccac
3961 agtacttaga accacaaagt gggaatcaag agaaaaacaa tgatcccacg agagatctat
4021 agatctatag atcatgagtg ggaggaatga gctggccctt aatttggttt tgcttgttta
4081 aattatgata tccaactatg aaacattatc ataaagcaat agtaaagagc cttcagtaaa
4141 gagcaggcat ttatctaatc ccaccccacc cccacccccg tagctccaat ccttccattc
4201 aaaatgtagg tactctgttc tcacccttct taacaaagta tgacaggaaa aacttccatt
4261 ttagtggaca tctttattgt ttaatagatc atcaatttct gcatcccggg gatctgatat
4321 catcgatgca tggggtcgtg cgctcctttc ggtcgggcgc tgcgggtcgt ggggcgggcg
4381 tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc
4441 ggcggtgacg gtgaagccga gccgctcgta gaaggggagg ttgtggggcg cggaggtctc
4501 caggaaggcg ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct
4561 gcccagaccc ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg
4621 ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga
4681 accgctcaac tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct
4741 ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat
4801 gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg
4861 gtccggatcg acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg
4921 tacggccctg gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat
4981 ggtaagcttc agctgctcga gatctagatg gatgcaggtc gaaaggcccg gagatgagga
5041 agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga
5101 ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc
5161 ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca
5221 aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac tggtgagacg
5281 tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct
5341 gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag tcggttggcg
5401 cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc
5461 cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg
5521 gtagaatttc gaggtcgaga tcctaagctt ggctggacgt aaactcctct tcagactagt
5581 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
5641 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
5701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
5761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
5821 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
5881 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
5941 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
6001 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
6061 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
6121 tgggaggtct atataagcag agctcgttta gtgaaccgtc agaattttgt aatacgactc
6181 actatagggc ggccgggaat tcgtcgactg gatccggtac cgaggagatc tgccgccgcg
6241 atcgccatga gcgagctgat caaggagaac atgcacatga agctgtacat ggagggcacc
6301 gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
6361 cagaccatga agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
6421 gctaccagct tcatgtacgg cagcaaagcc ttcatcaacc acacccaggg catccccgac
6481 ttctttaagc agtccttccc tgagggcttc acatgggaga gaatcaccac atacgaagac
6541 gggggcgtgc tgaccgctac ccaggacacc agcttccaga acggctgcat catctacaac
6601 gtcaagatca acggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacacgc
6661 ggctgggagg ccaacaccga gatgctgtac cccgctgacg gcggcctgag aggccacagc
6721 cagatggccc tgaagctcgt gggcgggggc tacctgcact gctccttcaa gaccacatac
6781 agatccaaga aacccgctaa gaacctcaag atgcccggct tccacttcgt ggaccacaga
6841 ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga gatggctgtg
6901 gccaagtact gcgacctccc tagcaaactg gggcacagat gaacgcgtac gcggccgctc
6961 gagcagaaac tcatctcaga agaggatctg gcagcaaatg atatcctgga ttacaaggat
7021 gacgacgata aggtttaaac ggccggccgc ggtcatagct gtttcctgaa cagatcccgg
7081 gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt
7141 gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt
7201 ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac
7261 ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct
7321 cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt
7381 gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg
7441 tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg
7501 gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat
7561 tttaaaataa ctataccagc aggaggacgt ccagacacag cataggctac ctggccatgc
7621 ccaaccggtg ccagagagga tcctgggagg gagagcttgg cagggggtgg gagggaaggg
7681 ggggatgcgt gacctgcccg gttctcagtg gccaccctgc gctaccctct cccagaacct
7741 gagctgctct gacgcggccg tctggtgcgt ttcactgatc ctggtgctgc agcttcctta
7801 cacttcccaa gaggagaagc agtttggaaa aacaaaatca gaataagttg gtcctgagtt
7861 ctaactttgg ctcttcacct ttctagtccc caatttatat tgttcctccg tgcgtcagtt
7921 ttacctgtga gataaggcca gtagccagcc ccgtcctggc agggctgtgg tgaggagggg
7981 ggtgtccgtg tggaaaactc cctttgtgag aatggtgcgt cctaggtgtt caccaggtcg
8041 tggccgcctc tactcccttt ctctttctcc atccttcttt ccttaaagag tccccagtgc
8101 tatctgggac atattcctcc gcccagagca gggtcccgct tccctaaggc cctgctctgt
8161 ctaga
//
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