| 质粒名称: | pHSV-TK |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 165801 |
| 存储实验室: | Koen Venken |
|
载体骨架基本信息 |
|
| 载体名称: | pUPD2 |
| 载体出品公司: | Clontech |
| 空载体大小: | 2955bp |
| 完整质粒大小: | 4989 bp |
| 载体类型: | Insect Expression |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH10B |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | HSV-TK |
| 物种来源: | Viral |
| 插入大小: | 52bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Unknown (unknown if destroyed) |
|
文献引用方法 |
|
| 材料和方法部分: | pHSV-TK was a gift from Koen Venken (Addgene plasmid # 165801 ; http://n2t.net/addgene:165801 ; RRID:Addgene_165801) |
| 参考文献部分: | Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila. Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT. Cell Rep. 2021 Sep 14;36(11):109700. doi: 10.1016/j.celrep.2021.109700. 10.1016/j.celrep.2021.109700 PubMed 34525356 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHSV-TK 3007 bp ds-DNA circular SYN 16-JUN-2021
DEFINITION Minimal viral terminator derived from Herpes Simplex 1 thymidine
kinase gene.
ACCESSION .
VERSION .
KEYWORDS pHSV-TK
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3007)
TITLE Multiplexed drug-based selection and counterselection genetic
manipulations in Drosophila
REFERENCE 2 (bases 1 to 3007)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 16, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3007
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 35..51
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 35..51
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 67..85
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 67..84
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(220..239)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(221..239)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(246..263)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(246..262)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
terminator 404..431
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
rep_origin 457..912
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(544..563)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 754..775
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 990..1094
/gene="bla"
/label=AmpR promoter
CDS 1095..1955
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1313..1332)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2126..2714
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2615..2634
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2868..2885
/label=L4440
/note="L4440 vector, forward primer"
terminator 2900..2971
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 cccgaaaggg gggccttttt tcgttttggt ccgacaggaa acagctatga ccaacggctc
61 tatggtattt aggtgacact atagaacgaa ttcactagtg attgggcgat gagtggtctc
121 gagcggaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccaagccga
181 gaccttgaga tcaatatcta aggtcctaag cggaattcac cctatagtga gtcgtattac
241 aattcactgg ccgtcgtttt acattgagaa gagaaaagaa aaccgccgat cctgtccacc
301 gcattactgc aaggtagtgg acaagaccgg cggtcttaag ttttttggct gaaagggtgg
361 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
421 gatggccttt ttgcgtttct acaatgttac gaatggacgc gccctgtagc ggcgcattaa
481 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
541 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
601 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
661 aaaaacttga ttagggtgat ggttcacgta gtgggccagc gccctgatag acggtttttc
721 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
781 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
841 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
901 cgcttacaat ttcctgatgc ggtattttct acttacgcat ctgtgcggta tttcacaccg
961 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
1021 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
1081 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
1141 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
1201 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
1261 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
1321 gcgcggtatt atcccgtatt gacgccgggc aagagcaact aggtcgccgc atacactatt
1381 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
1441 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
1501 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
1561 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
1621 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
1681 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
1741 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
1801 gtgagcgtgg gtcgcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
1861 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
1921 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
1981 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
2041 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
2101 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
2161 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
2221 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
2281 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
2341 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
2401 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
2461 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
2521 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
2581 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
2641 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
2701 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
2761 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
2821 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
2881 gcgaggaagc ggaaggtcag agagcgttca ccgacaaaca acagataaaa cgaaaggccc
2941 agtctttcga ctgagccttt cgttttattt gatgcctgct cggtaccaaa ttccagaaaa
3001 gaggcct
//
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