| 质粒名称: | pSFFV_mNG2(11)1-10 (pSPIN, pSC101* backbone) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 82610 |
| 存储实验室: | MTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVAKHFKPHGFSSDKAKAGSSE WLAVDGLVSPSNNSKEDAFSGTDWMLEKMDLKEFDLDALLGIDDLETMPDDLLTTLDDTC DLFAPLVQETNKQPPQTVNPIGHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTPDHSFSL ELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRG SPNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKR AEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP |
|
载体骨架基本信息 |
|
| 载体名称: | pHR-SFFV |
| 载体出品公司: | Clontech |
| 空载体大小: | 9000bp |
| 完整质粒大小: | 9639bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | NeonGreen2(1-10) |
| 物种来源: | Synthetic |
| 插入大小: | 624bp |
| 基因突变: | K128M, S142T, R150M, G172V and K213M |
| GenBank编号 | JN247409 |
| 启动子: | SFFV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI (not destroyed) |
| 3’克隆位点: | siteNotI (not destroyed) |
| 5’测序引物 | GCTTCTGCTTCCCGAGCTCTA |
| 3’测序引物 | GCAGCGTATCCACATAGCGTA |
|
文献引用方法 |
|
| 材料和方法部分: | pSFFV_mNG2(11)1-10 was a gift from Bo Huang (Addgene plasmid # 82610 ; http://n2t.net/addgene:82610 ; RRID:Addgene_82610) |
| 参考文献部分: | Improved split fluorescent proteins for endogenous protein labeling. Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B. Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8. 10.1038/s41467-017-00494-8 PubMed 28851864 |
买家导航
pSFFV_mNG2(11)1-10载体质粒基本信息
pSFFV_mNG2(11)1-10质粒图谱载体图谱和pSFFV_mNG2(11)1-10载体序列质粒序列多克隆位点信息
1-10质粒图谱.png)
pSFFV_mNG2(11)1-10质粒载体简介
pSFFV_mNG2(11)1-10质粒序列载体序列
LOCUS pSFFV_mNG2(11)1-10 9239 bp DNA circular SYN 25-AUG-2024
DEFINITION Lentiviral vector of constitutive expression of mNeonGreen2(1-10).
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9239)
AUTHORS Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
TITLE Improved split fluorescent proteins for endogenous protein labeling.
JOURNAL Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
PUBMED 28851864
REFERENCE 2 (bases 1 to 9239)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: "
10.1038/s41467-017-00494-8"
FEATURES Location/Qualifiers
source 1..9239
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 145..378
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 546..680
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(570..589)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 624..643
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS complement(1105..1125)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
intron 1255..1320
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
promoter complement(2454..2783)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2468..2603
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2768..2788
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
primer_bind complement(2858..2875)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3029..3617)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3109..3128)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3788..4648)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4411..4430
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4649..4753)
/gene="bla"
/label=AmpR promoter
primer_bind 4821..4839
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(4877..4899)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 4999..5018
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5232..5251
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 5541..5559
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 5541..5558
/label=SP6
/note="SP6 promoter, forward primer"
LTR 5584..5817
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind 5907..5923
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 5908..5924
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 5927..6515
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS 6033..6044
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind 6442..6462
/label=WPRE-R
/note="WPRE, reverse primer"
regulatory 6952..6961
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
promoter complement(7291..7698)
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
misc_feature 7851..7968
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(8253..8297)
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 8482..8715
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature join(9212..9239,1..98)
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
ORIGIN
1 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc
61 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct
121 ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga
181 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
241 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
301 agatctggtc taaccagaga aacccagtac aagcaaaaag cagatcttgt cttcgttggg
361 agtgaattag cccttccaac tactaagttt gtagtacata tttaacaaat acaatttctt
421 taaaatgaaa ataattcaga ggaatcacag gtttagagta aatgaaacca caggtaattg
481 gcagtggtaa tagggtatgg ggtgggaagt ttgggatgat tttggttagc ttgagttatc
541 cagttgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
601 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
661 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
721 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
781 atgatctcta gtcaaggcac tatacatcaa atattcctta ttaacccctt tacaaattaa
841 aaagctaaag gtacacaatt tttgagcata gttattaata gcagacactc tatgcctgtg
901 tggagtaaga aaaaacagta tgttatgatt ataactgtta tgcctactta taaaggttac
961 agaatatttt tccataattt tcttgtatag cagtgcagct ttttcctttg tggtgtaaat
1021 agcaaagcaa gcaagagttc tattactaaa cacagcatga ctcaaaaaac ttagcaattc
1081 tgaaggaaag tccttggggt cttctacctt tctcttcttt tttggaggag tagaatgttg
1141 agagtcagca gtagcctcat catcactaga tggcatttct tctgagcaaa acaggttttc
1201 ctcattaaag gcattccacc actgctccca ttcatcagtt ccataggttg gaatctaaaa
1261 tacacaaaca attagaatca gtagtttaac acattataca cttaaaaatt ttatatttac
1321 cttagagctt taaatctctg taggtagttt gtccaattat gtcacaccac agaagtaagg
1381 ttccttcaca aagatccggg gcccactcat aaatccagtt gccgccacgg tagccaatca
1441 ccgtatcgta taaatcatcg tcggtacgtt cggcatcgct catcacaata cgtgcctgga
1501 cgtcgaggat ttcgcgtggg tcaatgccgc gccagatcca catcagacgg ttaatcatgc
1561 gataccagtg agggatggtt ttaccatcaa gggccgactg cacaggcggt tgtgcgccgt
1621 gattaaagcg gcggactagc gtcgaggttt caggatgttt aaagcggggt ttgaacaggg
1681 tttcgctcag gtttgcctgt gtcatggatg cagcctccag aatacttact ggaaactatt
1741 gtaacccgcc tgaagttaaa aagaacaacg cccggcagtg ccaggcgttg aaaagattag
1801 cgaccggaga ttggcgggac gaatacgacg cccatatccc acggctgttc aatccaggta
1861 tcttgcggga tatcaacaac atagtcatca accagcggac gaccagccgg ttttgcgaag
1921 atggtgacaa agtgcgcttt tggatacatt tcacgaatcg caaccgcagt accaccggta
1981 tccaccaggt catcaataac gatgaagcct tcgccatcgc cttctgcgcg tttcagcact
2041 ttaagctcgc gctggttgtc gtgatcgtag ctggaaatac aaacggtatc gacatgacga
2101 atacccagtt cacgcgccag taacgcaccc ggtacagacc gccacggctt acggcaataa
2161 tgcctttcca ttgttcagaa ggcatcagtc ggcttgcgag tttacgtgca tggatctgca
2221 acatgtccca ggtgacgatg tatttttcgc tcatgtgaag tgtcccagcc tgtttatcta
2281 cggcttaaaa agtgttcgag gggaaaatag gttgcgcgag attatagaga tctggcgcac
2341 taaaaaccag tatttcacat gagtccgcgt ctttttacgc actgcctctc cctgacgcgg
2401 gataaagtgg tattctcaaa catatctcgc aagcctgtct tgtgtccaag ctttttgcaa
2461 aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg
2521 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
2581 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
2641 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
2701 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
2761 tttccacacc ctaactgaca cacattccac agctgcatta atgaatcggc caacgcgcgg
2821 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
2881 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
2941 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
3001 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
3061 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
3121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
3181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
3241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
3301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
3361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
3421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
3481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
3541 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
3601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
3661 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
3721 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
3781 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
3841 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
3901 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
3961 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
4021 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
4081 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
4141 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
4201 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
4261 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
4321 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
4381 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
4441 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
4501 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
4561 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
4621 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
4681 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
4741 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
4801 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
4861 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
4921 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
4981 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac cattcgacgc
5041 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
5101 accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
5161 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
5221 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
5281 atgccggcca cgatgcgtcc ggcgtagagg atctggctag cgatgaccct gctgattggt
5341 tcgctgacca tttccgggtg cgggacggcg ttaccagaaa ctcagaaggt tcgtccaacc
5401 aaaccgactc tgacggcagt ttacgagaga gatgataggg tctgcttcag taagccagat
5461 gctacacaat taggcttgta catattgtcg ttagaacgcg gctacaatta atacataacc
5521 ttatgtatca tacacatacg atttaggtga cactatagaa tacacggaat taattctaga
5581 tgctgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
5641 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
5701 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
5761 acccagtaca agcaaaaagc agatcttgtc ttcgttggga gtgaattagc ccttccagtc
5821 cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
5881 aaggtaccga gctcgaatta attccctcga ggtcgacggt atcgatgcgg ggaggcggcc
5941 caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc
6001 ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag
6061 gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat
6121 ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg
6181 gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg
6241 gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca
6301 gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac
6361 caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa
6421 ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc
6481 aatctttcac aaattttgta atccagaggt tgattatcga taagcttgat atcaagcttg
6541 catgcctgca ggtcgactct agagtcgcgg ccgcttacat ggagtgcttg agctccgtct
6601 tacggaacac gtacatcggc tggttcttca gatagttagc cgccattggc ttggcaaagg
6661 tgtaggtggt ccgcgcagtg ctccggtagc gtttgccatt tacagtggtg taactccact
6721 taaaggtact gatgatggtt ttgtcgttgg ggtaagtctt cttcgacatg caccagtccg
6781 cagcggtcag cgtgttggtc atcacaggac cgtcagcagg gaaaccagtc cccatcacct
6841 gggcctctcc tttgatgtgg cttccctcgt aggtgtagcg gtagttaaca gtaagggagg
6901 caccatcttc aaactgcatt gtgcgatgga cttggtatcc ggagccatct accatggcgg
6961 cctggaaagg cgacatcccg tcagggtagg gcaggtactg atggaagcca tacccgatat
7021 gagggaccag aatccagggg gagaactgga ggtcaccctt ggtggacttc aggtttaact
7081 cctcataacc atcatttgga ttgccggtac cctgacccac catgtcaaag tccacaccgt
7141 tgatggagcc aaagatgtgt aactcatgag tcgctgggag agaggccatg ttatcctcct
7201 cacccttgct caccatggat cccgggcccg cggtaccgca agcttgatat cctgcagacg
7261 cgtgagaatt ctcgagagct ccagatcccc cccgggcgac tcagtctgtc ggaggactgg
7321 cgcgccgagt gaggggttgt gagctctttt atagagctcg ggaagcagaa gcgcgcgaac
7381 agaagcgaga agcaggctga ttggttaatt caaataaggc gcagggtcat ttcaggtcct
7441 tgggggagcc tggaaacatc tgatgggtct taagaaactg ctgagggttg ggccatatct
7501 ggggaccatc tgttcttggc ctcgggccgg ggccgaaact gcggtgacca tctgttcttg
7561 gccccgggcc ggggccgaaa ctgctcaccg cagatatcct gtttggccca acgttagcta
7621 ttttcatgta cccgcccttg atctgaactt ctctattctt ggtttggtat ttttccatgc
7681 cttgcaaaat ggcgttactg cagctagctt gccaaaccta caggtggggt ctttcattcc
7741 cccctttttc tggagactaa ataaaatctt ttattttatc ggggctgcag gaattcgata
7801 tcaagcttat cgatccaaac tggatctctg ctgtccctgt aataaacccg aaaattttga
7861 atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact
7921 attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat
7981 actgccattt ggcgataccg tcgagatccg ttcactaatc gaatggatct gtctctgtct
8041 ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg
8101 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
8161 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
8221 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
8281 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
8341 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
8401 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
8461 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
8521 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
8581 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
8641 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
8701 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
8761 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
8821 ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg gccgcttgct
8881 gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt
8941 cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc
9001 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
9061 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
9121 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
9181 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttc
//
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pSFFV_mNG2(11)1-10质粒载体应用举例
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