| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141387 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | 9096 bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-orf6 |
| 物种来源: | SARS-CoV-2 |
| 基因突变: | human codon optimized |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (C terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141387 ; http://n2t.net/addgene:141387 ; RRID:Addgene_141387) |
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro 9096 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9096)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 9096)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9096
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..1182
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 231..1173
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1130..1150
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 1414..1437
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 1474..1497
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 1509..2082
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1676..1693)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 1903..1922
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 2115..2714
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2115..2134)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2611..2631
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 2728..3316
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2781..2801)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3199..3210)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3523..4156
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(4284..4300)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4284..4300)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4297..4319)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4308..4324
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4332..4362)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4377..4398
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4515..4532)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4686..5274)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4766..4785)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5445..6305)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6068..6087
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6306..6410)
/gene="bla"
/label=AmpR promoter
polyA_signal 6458..6592
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6495..6514)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6549..6568
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 6760..7393
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 7440..7565
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8062..8295
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8480..8524
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 8787..8902
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
ORIGIN
1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatctat ttccggtgaa
1201 ttcgccgcca ccatgtttca tcttgtcgat tttcaagtta cgattgcgga gatactcctc
1261 ataatcatgc ggacatttaa ggtttccatt tggaaccttg attacattat taacctgatt
1321 atcaagaatc tctccaagag cctgaccgag aataagtatt ctcagttgga cgaggagcag
1381 cctatggaaa tagacctcga aggcggcggg ggatggagcc atccacaatt cgagaaaggc
1441 ggtggttcag gaggaggtag cgggggtgga tcatggtcac atccgcagtt tgaaaagtaa
1501 ggatcccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
1561 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
1621 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
1681 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
1741 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
1801 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
1861 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
1921 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
1981 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
2041 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa
2101 ggagacgacc ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
2161 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
2221 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
2281 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
2341 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
2401 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
2461 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
2521 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
2581 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
2641 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
2701 agcccggtgc ctagacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
2761 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
2821 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
2881 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
2941 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
3001 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
3061 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
3121 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
3181 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
3241 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
3301 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
3361 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
3421 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
3481 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
3541 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
3601 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
3661 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
3721 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
3781 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
3841 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
3901 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
3961 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
4021 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
4081 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
4141 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
4201 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct
4261 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
4321 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
4381 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
4441 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
4501 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
4561 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
4621 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
4681 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
4741 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
4801 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
4861 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
4921 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
4981 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
5041 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
5101 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
5161 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
5221 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
5281 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
5341 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
5401 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
5461 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
5521 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
5581 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
5641 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
5701 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
5761 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
5821 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
5881 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
5941 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
6001 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
6061 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
6121 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
6181 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
6241 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
6301 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
6361 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
6421 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
6481 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
6541 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
6601 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
6661 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
6721 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
6781 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
6841 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
6901 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
6961 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
7021 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
7081 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
7141 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
7201 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
7261 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
7321 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
7381 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
7441 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
7501 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
7561 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
7621 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
7681 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
7741 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
7801 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
7861 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
7921 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
7981 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
8041 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
8101 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
8161 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
8221 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
8281 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
8341 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
8401 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
8461 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
8521 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
8581 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
8641 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
8701 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
8761 agtgaacgga tctcgacggt atcgccttta aaagaaaagg ggggattggg gggtacagtg
8821 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
8881 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttatc
8941 gatgagtaat tcatacaaaa ggactcgccc ctgccttggg gaatcccagg gaccgtcgtt
9001 aaactcccac taacgtagaa cccagagatc gctgcgttcc cgccccctca cccgcccgct
9061 ctcgtcatca ctgaggtgga gaagagcatg cgtgag
//
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