| 质粒名称: | 3xAP1pGL3 (3xAP-1 in pGL3-basic) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 40342 |
| 存储实验室: | Alexander Dent |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3-basic |
| 载体出品公司: | Promega |
| 空载体大小: | 4818 bp |
| 完整质粒大小: | 4914 bp |
| 载体类型: | 哺乳细胞表达, Luciferase |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | 3xAP-1、Three canonical AP-1 binding sites (TGACTCA) |
| 基因突变: | Contains three canonical AP-1 binding sites (TGACTCA) upstream of a minimal promoter fragment containing a TATA box |
| 标签/融合蛋白: | Luciferase (C terminal on backbone) |
| 克隆方法: | 未知 |
| 5’克隆位点: | AgeI(未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | RVprimer3 |
| 3’测序引物 |
LucNrev
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: | 3xAP1pGL3 (3xAP-1 in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40342 ; http://n2t.net/addgene:40342 ; RRID:Addgene_40342) |
| 参考文献部分: |
Repression of AP-1 function: a mechanism for the regulation of Blimp-1 expression and B lymphocyte differentiation by the B cell lymphoma-6 protooncogene. Vasanwala FH, Kusam S, Toney LM, Dent AL. J Immunol. 2002 Aug 15;169(4):1922-9. PubMed 12165517
|
买家导航
3xAP1pGL3(3xAP-1 in pGL3-basic)载体质粒基本信息
3xAP1pGL3(3xAP-1 in pGL3-basic)质粒图谱载体图谱和3xAP1pGL3(3xAP-1 in pGL3-basic)载体序列质粒序列多克隆位点信息
质粒图谱.png)
3xAP1pGL3(3xAP-1 in pGL3-basic)质粒载体简介
3xAP1pGL3(3xAP-1 in pGL3-basic)质粒序列载体序列
LOCUS 3xAP1pGL3 (3xAP-1 in pGL3-basic) 4914 bp DNA circular SYN 25-AUG-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4914)
AUTHORS Vasanwala FH, Kusam S, Toney LM, Dent AL
TITLE Repression of AP-1 function: a mechanism for the regulation of
Blimp-1 expression and B lymphocyte differentiation by the B cell
lymphoma-6 protooncogene.
JOURNAL J Immunol. 2002 Aug 15;169(4):1922-9.
PUBMED 12165517
REFERENCE 2 (bases 1 to 4914)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J
Immunol."; date: "2002-08-15"; volume: "169(4)"; pages: "1922-9"
FEATURES Location/Qualifiers
source 1..4914
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 178..187
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 184..1836
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(250..268)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 1877..1998
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1888..1907)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1942..1961
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2246..2263)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2417..3005)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2497..2516)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3176..4036)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3799..3818
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4037..4141)
/gene="bla"
/label=AmpR promoter
rep_origin 4168..4623
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4255..4274)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4465..4486
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 4754..4802
/note="synthetic polyadenylation signal"
misc_feature 4816..4907
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 4856..4875
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggtaccgagc tcttacgcgt gctagcccgg gcactgactc atcaagcact gactcatcaa
61 gcactgactc atcaagggac tcagggaggg aaactctttg cagataaata ctcctcagca
121 gccggcactc gagatctgcg atctaagtaa gcttggcatt ccggtactgt tggtaaagcc
181 accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat
241 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
301 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg
361 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
421 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
481 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc
541 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
601 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag
661 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
721 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct
781 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc
841 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
901 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
961 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat
1021 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact
1081 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc
1141 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga
1201 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
1261 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
1321 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc
1381 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
1441 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag
1501 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc
1561 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa
1621 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
1681 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
1741 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
1801 ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agagtcgggg cggccggccg
1861 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
1921 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
1981 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
2041 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag
2101 gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
2161 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
2221 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
2281 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
2341 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
2401 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
2461 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
2521 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
2581 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
2641 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
2701 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
2761 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
2821 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
2881 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
2941 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
3001 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
3061 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
3121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
3181 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
3241 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
3301 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
3361 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
3421 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
3481 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
3541 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
3601 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
3661 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
3721 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
3781 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
3841 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
3901 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
3961 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
4021 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
4081 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
4141 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
4201 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
4261 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
4321 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
4381 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
4441 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
4501 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
4561 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
4621 tttgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
4681 cgctattacg ccagcccaag ctaccatgat aagtaagtaa tattaaggta cgggaggtac
4741 ttggagcggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
4801 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
4861 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctatc gata
//
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