| 质粒名称: | pLeAPS-GFP-blast |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 182230 |
| 存储实验室: | Michael Birnbaum |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti |
| 载体出品公司: | Clontech |
| 空载体大小: | 8066bp |
| 完整质粒大小: | 9248bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Blasticidin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | GFP-P2A-Blasticidin |
| 插入大小: | 1182 bp |
| 启动子: | CMV |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | NA |
| 3’测序引物 | NA |
|
文献引用方法 |
|
| 材料和方法部分: | pLeAPS-GFP-blast was a gift from Michael Birnbaum (Addgene plasmid # 182230 ; http://n2t.net/addgene:182230 ; RRID:Addgene_182230) |
| 参考文献部分: | Antigen identification and high-throughput interaction mapping by reprogramming viral entry. Dobson CS, Reich AN, Gaglione S, Smith BE, Kim EJ, Dong J, Ronsard L, Okonkwo V, Lingwood D, Dougan M, Dougan SK, Birnbaum ME. Nat Methods. 2022 Apr;19(4):449-460. doi: 10.1038/s41592-022-01436-z. Epub 2022 Apr 8. 10.1038/s41592-022-01436-z PubMed 35396484 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLeAPS-GFP-blast 9248 bp ds-DNA circular SYN 15-APR-2022
DEFINITION Lentiviral transfer plasmid for the LeAPS packaging system; encodes
GFP-P2A-blasticidin transgene.
ACCESSION .
VERSION .
KEYWORDS pLeAPS-GFP-blast
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9248)
TITLE Antigen identification and high-throughput interaction mapping by
reprogramming viral entry
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9248)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 15, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9248
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 46..474
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(133..152)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 343..364
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(483..503)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 648..782
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 709..728
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 844..891
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 910..1284
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 1414..1535
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1451..1470)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1505..1524
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(1584..1600)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1584..1600)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1597..1619)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1608..1624
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1632..1662)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1677..1698
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1815..1832)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1986..2574)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2066..2085)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2745..3605)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3368..3387
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3606..3710)
/gene="bla"
/label=AmpR promoter
primer_bind complement(3785..3804)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 3976..4355
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4357..4555
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 4573..4753
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 4800..4925
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 5418..5651
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 5836..5880
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 6178..6295
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 6388..6599
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
regulatory 6618..6627
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 6624..7340
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 6624..7340
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(6669..6690)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(6930..6949)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 7277..7298
/label=EGFP-C
/note="EGFP, forward primer"
CDS 7350..7406
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 7407..7805
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 7830..8418
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7883..7903)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8301..8312)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(8421..8437)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8422..8438)
/label=pBluescriptKS
/note="For pBluescript vector"
promoter 8596..8794
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 8812..8992
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(9018..9035)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9024..9248
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
ORIGIN
1 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
61 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
121 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
181 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
241 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
301 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
361 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
421 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
481 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
541 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
601 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
661 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
721 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
781 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
841 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
901 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
961 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
1021 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
1081 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
1141 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
1201 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
1261 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
1321 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
1381 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
1441 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
1501 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
1561 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
1621 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
1681 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
1741 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
1801 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
1861 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
1921 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
1981 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
2041 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
2101 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
2161 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
2221 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
2281 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
2341 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
2401 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
2461 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
2521 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
2581 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
2641 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
2701 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
2761 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
2821 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
2881 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
2941 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
3001 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
3061 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
3121 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
3181 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
3241 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
3301 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
3361 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
3421 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
3481 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
3541 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
3601 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
3661 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
3721 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
3781 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
3841 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
3901 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
3961 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
4021 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
4081 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
4141 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
4201 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
4261 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
4321 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
4381 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
4441 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
4501 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
4561 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
4621 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
4681 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
4741 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
4801 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
4861 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
4921 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
4981 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
5041 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
5101 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
5161 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
5221 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
5281 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
5341 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
5401 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
5461 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
5521 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
5581 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
5641 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
5701 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
5761 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
5821 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
5881 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
5941 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
6001 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
6061 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
6121 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
6181 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
6241 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
6301 ttattacagg gacagcagag atccagtttg gttaattagc tagctaggtc ttgaaaggag
6361 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
6421 agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact
6481 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
6541 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
6601 accggttcta gagcgctgcc accatggtga gcaagggcga ggagctgttc accggggtgg
6661 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
6721 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
6781 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
6841 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
6901 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
6961 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
7021 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
7081 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
7141 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
7201 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
7261 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
7321 gcatggacga gctgtacaag ggatccggcg caacaaactt ctctctgctg aaacaagccg
7381 gagatgtcga agagaatcct ggaccgatgg ccaagccttt gtctcaagaa gaatccaccc
7441 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
7501 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
7561 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
7621 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
7681 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
7741 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
7801 gctaagaatt cgatatcaag cttatcggta atcaacctct ggattacaaa atttgtgaaa
7861 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
7921 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
7981 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
8041 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
8101 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
8161 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
8221 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
8281 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
8341 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
8401 tttgggccgc ctccccgcat cgataccgtc gacctcgagg tcgactttaa gaccaatgac
8461 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
8521 aattcactcc caacgaagac aagatttcac cattatcgtt tcagacccac ctcccaaccc
8581 cgaggggacc cagagtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
8641 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
8701 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
8761 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
8821 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
8881 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
8941 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
9001 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
9061 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
9121 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
9181 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
9241 ctctatgg
//
